scholarly journals Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo

2014 ◽  
Vol 30 (3) ◽  
pp. 305-312 ◽  
Author(s):  
Indrani Singha ◽  
Subir Kumar Das
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Liver Tissue ◽  
Ex Vivo ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenging Properties ◽  
Grape Cultivars

Free Radical Scavenging potential of different extracts of Tabebuia roseo-alba (Ridl) Sand leaves

2021 ◽  
pp. 4801-4807
Author(s):  
Suseela V. ◽  
Sushmita L. ◽  
Bharatkumar R. ◽  
Nirmaladevi R.
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Age Related ◽  
Radical Scavenging Properties ◽  
Hydroxyl Hydrogen

The present study was carried out to elucidate the in vitro free radical scavenging potential of various extracts of Tabebuia roseo-alba leaves. Assays for radical scavenging, such as DPPH, ABTS+, hydroxyl, hydrogen peroxide, superoxide, nitric oxide assay and reducing power activity were performed using standard protocols and the results were compared with standard ascorbic acid. Among the various extracts used the ethanolic extract of T. roseo-alba exhibited efficient scavenging potential with lowest EC50 value proving its antioxidant potential. Leaves of T. roseo-alba have strong free radical scavenging properties and thus can be used as a potential antioxidant to resolve diseases that are associated with oxidative stress including diabetes and other age related disorders.

scholarly journals In Vitro and In Vivo Antioxidant Properties of Taraxacum officinale in Nω-Nitro-l-Arginine Methyl Ester (L-NAME)-Induced Hypertensive Rats

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 309
Author(s):  
Olukayode O. Aremu ◽  
Adebola O. Oyedeji ◽  
Opeoluwa O. Oyedeji ◽  
Benedicta N. Nkeh-Chungag ◽  
Constance R. Sewani Rusike
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Methyl Ester ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hypertensive Rats ◽  
Total Antioxidant

Oxidative stress has gained attention as one of the fundamental mechanisms responsible for the development of hypertension. The present study investigated in vitro and in vivo antioxidant effects of 70% ethanol-water (v/v) leaf and root extracts of T. officinale (TOL and TOR, respectively). Total phenolic and flavonoid content of plant extracts were assessed using Folin Ciocalteau and aluminium chloride colorimetric methods; while, 2,2-diphenyl-1-picrlhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) protocols were used to determine the free radical scavenging and total antioxidant capacities (TAC), respectively. The in vivo total antioxidant capacity and malondialdehyde acid (MDA) levels for lipid peroxidation tests were performed on organ homogenate samples from Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive rats treated with leaf extract, TOL (500 mg/kg/day) and TOR (500 mg/kg/day) for 21 days. Results showed that compared to TOR, TOL possessed significantly higher (p < 0.01) polyphenol (4.35 ± 0.15 compared to 1.14 ± 0.01) and flavonoid (23.17 ± 0.14 compared to 3 ± 0.05) content; free radical scavenging activity (EC50 0.37 compared to 1.34 mg/mL) and total antioxidant capacities (82.56% compared to 61.54% ABTS, and 156 ± 5.28 compared to 40 ± 0.31 FRAP) and both extracts showed no toxicity (LD50 > 5000 mg/kg). TOL and TOR significantly (p < 0.01) elevated TAC and reduced MDA levels in targets organs. In conclusion, T. officinale leaf extract possesses significant anti-oxidant effects which conferred significant in vivo antioxidant protection against free radical-mediated oxidative stress in L-NAME-induced hypertensive rats.

scholarly journals In vitro and ex vivo free radical scavenging activities of carazostatin, carbazomycin B and their derivatives.

1993 ◽  
Vol 46 (12) ◽  
pp. 1859-1865 ◽  
Author(s):  
SHINICHIRO KATO ◽  
TOMIKO KAWASAKI ◽  
TETSURO URATA ◽  
JUNICHIRO MOCHIZUKI
Keyword(s):  
Free Radical ◽  
Ex Vivo ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activities

scholarly journals Non-clinical assessment of lubrication and free radical scavenging of an innovative non-animal carboxymethyl chitosan biomaterial for viscosupplementation: An in-vitro and ex-vivo study

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0256770
Author(s):  
Jean-Michel Vandeweerd ◽  
Bernado Innocenti ◽  
Guillem Rocasalbas ◽  
Sandrine Emilia Gautier ◽  
Pierre Douette ◽  
...  
Keyword(s):  
Free Radical ◽  
Clinical Performance ◽  
Ex Vivo ◽  
Radical Scavenging ◽  
Carboxymethyl Chitosan ◽  
Free Radical Scavenging ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

Objective Lubrication and free radical scavenging are key features of biomaterials used for viscosupplementation (VS) of joints affected by osteoarthritis (OA). The objective of this study was to describe the non-clinical performance characterization of KiOmedine® CM-Chitosan, a non-animal carboxymethyl chitosan, in order to assess its intended action in VS and to compare it to existing viscosupplements based on crosslinked hyaluronan (HA) formulations. Method The lubrication capacity of the tested viscosupplements (VS) was evaluated in-vitro and ex-vivo. In-vitro, the coefficient of friction (COF) was measured using a novel tribological system. Meanwhile, an ex-vivo biomechanical model in ovine hindlimbs was developed to assess the recovery of join mobility after an intra-articular (IA) injection. Free radical scavenging capacity of HA and KiOmedine® CM-Chitosan formulations was evaluated using the Trolox Equivalent Antioxidant Capacity (TEAC) assay. Results In the in-vitro tribological model, KiOmedine® CM-Chitosan showed high lubrication capacity with a significant COF reduction than crosslinked HA formulations. In the ex-vivo model, the lubrication effect of KiOmedine® CM-Chitosan following an IA injection in the injured knee was proven again by a COF reduction. The recovery of joint motion was optimal with an IA injection of 3 ml of KiOmedine® CM-Chitosan, which was significantly better than the crosslinked HA formulation at the same volume. In the in-vitro TEAC assay, KiOmedine® CM-Chitosan showed a significantly higher free radical scavenging capacity than HA formulations. Conclusion Overall, the results provide a first insight into the mechanism of action in terms of lubrication and free radical scavenging for the use of KiOmedine® CM-Chitosan as a VS treatment of OA. KiOmedine® CM-Chitosan demonstrated a higher capacity to scavenge free radicals, and it showed a higher recovery of mobility after a knee lesion than crosslinked HA formulations. This difference could be explained by the difference in chemical structure between KiOmedine® CM-Chitosan and HA and their formulations.

scholarly journals Phytochemicals, Total Phenolic and Flavonoid Contents and Radical Scavenging Activities of Crotalaria Retusa, Clerodendrum Volubile Leaf Extracts and Biogas Slurry of Cassava Peels

2021 ◽  
Author(s):  
Oluwaseyi Olaniyan ◽  
Ebenezer Ajayi ◽  
Oluwaseun Oladele ◽  
Simeon Adenrele ◽  
Johnson Oladele ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Biogas Slurry ◽  
Promising Therapeutic Approach ◽  
Phenolic And Flavonoid

Abstract Well established are the destructive effects of cellular oxidative damage due to oxidative stress on animal and human health. The prophylactic use of plant-derived bioactive agents in the amelioration and treatment of chronic diseases induced by free radical and oxidative stress is a promising therapeutic approach. This present study investigates the phytochemical constituents, in vitro antioxidant and free radical scavenging abilities of Crotalaria retusa leaves, Clerodendrum volubile leaves and cassava biogas slurry. The total phenolic and flavonoid contents of the extracts were also determined. The phytochemicals, phenolic and flavonoid contents and antioxidant potentials of the extracts, including reducing power, inhibition of hydrogen peroxide, hydroxyl radical production, and DPPH radical scavenging, were performed using standard assays. Our results show terpenoids, saponins, cardiac glycosides, flavonoids and tannins in the various extracts. In vitro, antioxidant assay results also reveal that the extracts' free radical scavenging abilities are significantly high compared with standard antioxidants used: tannic and ascorbic acids. The results also show that the extracts demonstrated elevated antioxidant activities positively correlated with the total flavonoid and phenolic contents. This work demonstrated that the extracts possessed significant antioxidant activities over standard ones, thus, suggesting the extracts as sources of natural antioxidants that could have considerable therapeutic relevance in preventing and inhibiting the progression of diseases associated with oxidative stress.

Oxidative stress induced by ochratoxin A in LLC-PK1 cell line and the chemoprotective effects of carnosic acid

2008 ◽  
Vol 1 (4) ◽  
pp. 469-474 ◽  
Author(s):  
S. Costa ◽  
A. Utan ◽  
E. Speroni ◽  
R. Cervellati ◽  
G. Piva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Ochratoxin A ◽  
Cell Damage ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Carnosic Acid ◽  
Starting Point ◽  
Pre Treatment ◽  
Radical Scavenging Properties

Mycotoxins are secondary metabolites of various moulds that contaminate several alimentary substrates. One of the most dangerous of these is ochratoxin A (OTA). Reactive oxygen species (ROS) play an important role in the toxicity mechanism of OTA, so the use of natural or synthetic free radical scavengers could be a potential chemopreventive strategy. Carnosic acid (CA) is the major polyphenolic compound present in rosemary plants. This work aimed to determine whether CA could counteract OTA-induced cell damage. Free radical scavenging properties of CA were chemically determined at pH 7.4. Cytotoxicity of CA and OTA on LLC-PK1 cells, and the protective effects of CA, were assessed using the Alamar Blue test. The effects in vitro of CA pre-treatment on the production of ROS, the DNA oxidation and the induction of apoptosis induced by OTA were studied. It was found that CA has free radical scavenging properties at both the considered pH values. Moreover, a pre-treatment of 24 h with 10, 20 and 30 µM CA is able to reduce OTA-induced cytotoxicity; a pre-treatment of 24 h with 20 and 30 µM CA achieved ROS reduction and with 30 µM CA decreased the OTA-induced increase of 8-OH-2'-deoxyguanosine and of DNA fragmentation in LLC-PK1. These findings suggest a starting point to develop alimentary strategies against OTAinduced cell damage. Moreover, our results provide further evidence that oxidative stress plays an important role in the OTA cytotoxicity mechanism.

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