scholarly journals Validating a High Performance Liquid Chromatography-Ion Chromatography (HPLC-IC) Method with Conductivity Detection After Chemical Suppression for Water Fluoride Estimation

2017 ◽  
Vol 33 (1) ◽  
pp. 86-90 ◽  
Author(s):  
Joseph Dian Bondu ◽  
R. Selvakumar ◽  
Jude Joseph Fleming
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ion Chromatography ◽  
High Performance ◽  
Conductivity Detection ◽  
Water Fluoride

scholarly journals New Analytical Approach for the Determination of Calcium Phosphate Dibasic and Tribasic in Processed Food by Comparison of Ion Chromatography with High-Performance Liquid Chromatography

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 248 ◽  
Author(s):  
Minjung Song ◽  
Juhee Park ◽  
Jihyun Lee ◽  
Heejae Suh ◽  
Hyunjung Lee ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Calcium Phosphate ◽  
Ion Chromatography ◽  
High Performance ◽  
Food Additives ◽  
Food Samples ◽  
Hplc Method ◽  
Processed Foods ◽  
Phosphate Ions

An analytical method to measure solubilized orthophosphate ions (HPO42− and PO43− ) from the water-insoluble food additives calcium phosphate dibasic (DCP) and calcium phosphate tribasic (TCP) in processed foods was optimized by comparing ion chromatography (IC) coupled with DS6 conductivity detector (Cond.) and high-performance liquid chromatography (HPLC) with Evaporative light scattering detector (ELSD) methods. The ion-pairing HPLC method could analyze calcium and phosphate ions successively. However, this method exhibited low reproducibility after approximately 48 hours of measurements. The IC method was established as an effective method of measuring orthophosphate ions with high reproducibility using distilled water and KOH solution as the mobile phase with a Dionex column. Matrix-based limit of detections (LOD) and limit of quantifications (LOQ) for snacks and cereals were estimated in the range of 0.01–0.91 µg/mL and 0.21–2.74 µg/mL, respectively. In inter-day and intra-day tests, the calculated precision (%RSD) and accuracy (recovery %) ranged from 0.5% to 6.6% and 82% to 117%, respectively, in both food samples. The levels of DCP or TCP could be analyzed in various positive food samples, and the developed IC method demonstrated good applicability in the analysis of DCP and TCP in collected processed foods.

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