scholarly journals Molecular diversity in the genus Nicotiana as revealed by randomly amplified polymorphic DNA

2008 ◽  
Vol 14 (4) ◽  
pp. 377-382 ◽  
Author(s):  
K. Siva Raju ◽  
M. Sheshumadhav ◽  
T. G. K. Murthy
Keyword(s):  
Molecular Diversity ◽  
Randomly Amplified Polymorphic Dna

Molecular diversity of small balsam populations in relation to site characteristics

2013 ◽  
Vol 8 (10) ◽  
pp. 1048-1061 ◽  
Author(s):  
Eugenija Kupcinskiene ◽  
Lina Zybartaite ◽  
Rasa Janulioniene ◽  
Judita Zukauskiene ◽  
Algimantas Paulauskas
Keyword(s):  
Molecular Diversity ◽  
Genetic Distances ◽  
Randomly Amplified Polymorphic Dna ◽  
Multiple Introductions ◽  
Habitat Features ◽  
Site Characteristics ◽  
Impatiens Parviflora ◽  
Geographical Distances ◽  
Favorable Conditions ◽  
Simple Sequence

AbstractClimatic shifts within recent decades created favorable conditions for invasive species flourishing in more Northern parts of Europe. Our study was aimed at evaluation of genetic variability and habitat features of Impatiens parviflora populations growing in Lithuania. Twenty one populations were selected and analysed using simple sequence repeat (SSR) and randomly amplified polymorphic DNA (RAPD) assays. Evaluated by SSRs, 315 individuals were all monomorphic and homozygous at 4 loci and heterozygous at 1 locus. RAPD analyses revealed that the percentage of polymorphic DNA loci (% P) per population ranged from 7 to 39% and genetic differentiation between populations was ΦPT=0.790 (P<0.01). Genetic distances among populations (0.135–0.426) correlated significantly with geographical distances (r=0.183; P<0.008). Populations in overmoistured soil contained higher % P (28.3) when compared to drier soil (18.7; P<0.05). All recorded populations were close to roads; their % P did not depend on proximity to buildings, light intensity or population size. Our RAPD analyses indicate multiple introductions of this species in Lithuania. Analyses of I. parviflora at SSR and RAPD loci show that the invasion process is reflected in genetic structure.

scholarly journals Molecular Diversity of Renibacterium salmoninarum Isolates Determined by Randomly Amplified Polymorphic DNA Analysis

2000 ◽  
Vol 66 (1) ◽  
pp. 435-438 ◽  
Author(s):  
T. Hilton Grayson ◽  
Franck A. Atienzar ◽  
Sarah M. Alexander ◽  
Lynne F. Cooper ◽  
Martyn L. Gilpin
Keyword(s):  
Molecular Diversity ◽  
Dna Analysis ◽  
Repeat Unit ◽  
Intergenic Spacer ◽  
23S Rrna ◽  
Sequence Variant ◽  
Randomly Amplified Polymorphic Dna ◽  
Renibacterium Salmoninarum ◽  
Its1 Sequence ◽  
Biological Source

ABSTRACT The molecular diversity among 60 isolates of Renibacterium salmoninarum which differ in place and date of isolation was investigated by using randomly amplified polymorphic DNA (RAPD) analysis. Isolates were grouped into 21 banding patterns which did not reflect the biological source. Four 16S-23S rRNA intergenic spacer (ITS1) sequence variations and two alleles of an exact tandem repeat locus, ETR-A, were the bases for formation of distinct groups within the RAPD clusters. This study provides evidence that the most common ITS1 sequence variant, SV1, possesses two copies of a 51-bp repeat unit at ETR-A and has been widely dispersed among countries which are associated with mainstream intensive salmonid culture.

Assessment of molecular diversity of greengram [Vigna radiata (L.) Wilzek] through RAPD

2016 ◽  
Author(s):  
Gunnjeet Kaur ◽  
Arunabh Joshi ◽  
Devendra Jain ◽  
Ganesh Rajamani ◽  
Divya Vyas
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Polymorphism Information Content ◽  
Vigna Radiata ◽  
Molecular Diversity ◽  
Rapd Analysis ◽  
Similarity Coefficient ◽  
Green Gram ◽  
Randomly Amplified Polymorphic Dna ◽  
Legume Crop

A total twenty three genotypes of green gram (Vigna radiata) were subjected to Randomly amplified polymorphic DNA (RAPD) analysis for molecular characterization. A total of 25 randomly selected decamers were screened, out of which only 15 generated 126 amplification products from which 117 bands were found polymorphic, the average polymorphism being 93.48%. The total number of amplified bands varied between 2 (primer OPP-09) to 17 (primer OPA-1) with an average of 9.5 bands per primer. The overall size of PCR amplified products ranged between 200 bp to 2900 bp. The average Polymorphism Information Content(PIC) was 0.32 ranging from 0.17 to 0.46. Primer OPA-01 and OPP-06 detected two unique bands ranged between 250 bp to 2500 bp in two genotypes (PUSA-672 and HUM-12). Jaccard's similarity coefficient values ranged from 0.28-0.90 with an average of 0.59. Based on dendrogram generated through UPGMA method and PCA, most of the genotypes got divided into four main clusters. Genotype EC-398885 lay far apart and thus showed maximum genetic distance. The assessment of genetic diversity is a prerequisite and important step for the improvement of any legume crop. Thus, present results of the present study could be further extrapolated to other green gram accessions in Vigna germplasm.

Morphological and Molecular Diversity of Benthic Cyanobacteria Communities Versus Environmental Conditions in Shallow, High Mountain Water Bodies in Eastern Pamir Mountains (Tajikistan)

2020 ◽  
Vol 67 (4) ◽  
pp. 286 ◽  
Author(s):  
Iwona Jasser ◽  
Iwona Kostrzewska-Szlakowska ◽  
Jan Kwiatowski ◽  
Dovutsho Navruzshoev ◽  
Małgorzata Suska-Malawska ◽  
...  
Keyword(s):  
Environmental Conditions ◽  
Molecular Diversity ◽  
Water Bodies ◽  
High Mountain ◽  
Benthic Cyanobacteria ◽  
Pamir Mountains

STUDI PERAKITAN KELAPA HIBRIDA GSK x DMT BERDASARKAN PENANDA RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA)

EUGENIA ◽  
2008 ◽  
Vol 14 (1) ◽  
Author(s):  
Semuel D. Runtunuwu ◽  
Hengky Novarianto ◽  
Heldering Tampake ◽  
Edy F. Lengkong
Keyword(s):  
Dna Marker ◽  
Rapd Marker ◽  
High Yield ◽  
Randomly Amplified Polymorphic Dna ◽  
Germination Time ◽  
Flower Production ◽  
Short Time

ABSTRACT   Runtunuwu, S.D. et al. 2008. Assembling Hybrid Coconut of GSK x DMT Based on RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA) Marker. Eugenia 14 (1) : 134-152.   The aimed of this research was : 1. assembling hybrid coconut GSK x DMT (Genjah Salak x Dalam Mapanget) that seeds growth was relatifly homogeneous based on RAPD (Randomly Amplified Polymorphic DNA) marker and 2. to found the assembling method of hybrid coconut that will produce massive seeds relatifely short time will homogeneous plant. It was 65 individu trees observe for the average of famale flower per bunch. The result was 25 individu of coconut GSK has the average flower production > 40 per bunch was analyze the homogeneous genetic with the RAPD marker. Based on the analyze RAPD that were 25 individu of GSK coconut trees have the same genetic average 88 % and 14 individu among that was 100 % have same genetic. Further more that 14 individu of GSK was crossing with the 3 individu of DMT that have high yield per year its was DMT 1188, 1172 and 781. Based on the evaluation for the color of buds, high of buds, the steam circle, the petiole color and the germination time of hybrid coconut seeds from the crossing of GSK x DMT 1188 produce more than    70 % seeds that have same genetic, also for crossing of GSK x DMT 1172 have 9 combination and have more than 70 % that same genetic, 10 combination from crossing GSK x DMT 781 have more than 80 % same seeds growth. Therefore, using the RAPD marker were successfully produced 28 crossing of the hybrid coconut GSK x DMT that have relatifly homogeneous seeds growth.   Keywords : assembling, hybrid coconut GSK x DMT, RAPD.

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