Abstract. Column-averaged volume mixing ratios of carbon dioxide (XCO2) during the period from January 2007 to May 2008 over Tsukuba, Japan, were derived by using CO2 concentration data observed by Japan Airlines Corporation (JAL) commercial airliners, based on the assumption that CO2 profiles over Tsukuba and Narita were the same. CO2 profile data for 493 flights on clear-sky days were analysed in order to calculate XCO2 with an ancillary dataset: Tsukuba observational data (by rawinsonde and a meteorological tower) or global meteorological data (NCEP and CIRA-86). The amplitude of seasonal variation of XCO2 (Tsukuba observational) from the Tsukuba observational data was determined by least-squares fit using a harmonic function to roughly evaluate the seasonal variation over Tsukuba. The highest and lowest values of the obtained fitted curve in 2007 for XCO2 (Tsukuba observational) were 386.4 and 381.7 ppm in May and September, respectively. The dependence of XCO2 on the type of ancillary dataset was evaluated. The average difference between XCO2 (global) from global climatological data and XCO2 (Tsukuba observational), i.e., the bias of XCO2 (global) based on XCO2 (Tsukuba observational), was found to be -0.621 ppm with a standard deviation of 0.682 ppm. The uncertainty of XCO2 (global) based on XCO2 (Tsukuba observational) was estimated to be 0.922 ppm. This small uncertainty suggests that the present method of XCO2 calculation using data from airliners and global climatological data can be applied to the validation of GOSAT products for XCO2 over airports worldwide.