Peracetic Acid Sanitation on Arugula Microgreens Contaminated with Surface-Attached and Internalized Tulane Virus and Rotavirus

ABSTRACT We determined the disinfection efficacy and inactivation mechanisms of peracetic acid (PAA)-based sanitizer using pH values relevant for vegetable sanitation against rotavirus (RV) and Tulane virus (TV; a human norovirus surrogate). TV was significantly more resistant to PAA disinfection than RV: for a 2-log10 reduction of virus titer, RV required 1 mg/liter PAA for 3.5 min of exposure, while TV required 10 mg/liter PAA for 30 min. The higher resistance of TV can be explained, in part, by significantly more aggregation of TV in PAA solutions. The PAA mechanisms of virus inactivation were explored by quantifying (i) viral genome integrity and replication using reverse transcription-quantitative PCR (RT-qPCR) and (ii) virus-host receptor interactions using a cell-free binding assay with porcine gastric mucin conjugated with magnetic beads (PGM-MBs). We observed that PAA induced damage to both RV and TV genomes and also decreased virus-receptor interactions, with the latter suggesting that PAA damages viral proteins important for binding its host cell receptors. Importantly, the levels of genome-versus-protein damage induced by PAA were different for each virus. PAA inactivation correlated with higher levels of RV genome damage than of RV-receptor interactions. For PAA-treated TV, the opposite trends were observed. Thus, PAA inactivates each of these viruses via different molecular mechanisms. The findings presented here potentially contribute to the design of a robust sanitation strategy for RV and TV using PAA to prevent foodborne disease. IMPORTANCE In this study, we examined the inactivation mechanisms of peracetic acid (PAA), a sanitizer commonly used for postharvest vegetable washing, for two enteric viruses: Tulane virus (TV) as a human norovirus surrogate and rotavirus (RV). PAA disinfection mechanisms for RV were mainly due to genome damage. In contrast, PAA disinfection in TV was due to damage of the proteins important for binding to its host receptor. We also observed that PAA triggered aggregation of TV to a much greater extent than RV. These studies demonstrate that different viruses are inactivated via different PAA mechanisms. This information is important for designing an optimal sanitation practice for postharvest vegetable washing to minimize foodborne viral diseases.

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Обеззараживание семян капусты от сосудистого бактериоза

Kartofel` i ovoshi ◽

10.25630/pav.2018.1.17457 ◽

2018 ◽

Author(s):

A.T. Orynbayev ◽

F.S. Dzhalilov

Keyword(s):

Peracetic Acid ◽

Biological Efficiency ◽

Black Rot ◽

Presowing Treatment

Приведены данные двухлетнего исследования по поиску эффективных препаратов для обеззараживания семян капусты от сосудистого бактериоза. Наилучший результат был получен при предпосевной обработке 0,15%-ной надуксусной кислотой. При этом биологическая эффективность составляла 94,1–97,6%, что значительно превышало соответствующий показатель в эталонном варианте – Фитолавин, ВРК, 0,2% (69,0–72,0%). Показано, что заблаговременная обработка семян НУК (за 550 суток до посева) не оказывала достоверного влияния на их лабораторную всхожесть.Presented data are the result of a two-year study aimed at identifying effective preparations for the disinfection of cabbage seeds from black rot. The best result was obtained by presowing treatment with 0.15% peracetic acid. In this case, the biological efficiency was 94.1–97.6%, which significantly exceeded the corresponding indicator in the reference variant – Phytolavin, VRK, 0.2% (69.0–72.0%). The study has not revealed any significant effect of advance treatment of seeds with peracetic acid (550 days before sowing) on their laboratory germination.

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Peracetic Acid (PAA) Disinfection - Upgrading WWTPs to Reduce Ecosystem Toxicity

Proceedings of the Water Environment Federation ◽

10.2175/193864715819556219 ◽

2015 ◽

Vol 2015 (6) ◽

pp. 318-327 ◽

Cited By ~ 1

Author(s):

Kati Bell ◽

Philip Block ◽

Janelle Amador

Keyword(s):

Peracetic Acid

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Disinfection of Wastewater with Peracetic Acid (PAA) and UV Combined Treatment: A Pilot Study

Proceedings of the Water Environment Federation ◽

10.2175/193864718824828344 ◽

2018 ◽

Vol 2018 (6) ◽

pp. 76-89

Author(s):

Achal Garg ◽

Vasudevan Namboodiri ◽

Bruce Smith ◽

Abdulaziz Al-Anazi ◽

Brindha Murugesan ◽

...

Keyword(s):

Pilot Study ◽

Peracetic Acid ◽

Combined Treatment

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Peracetic Acid Disinfection

Proceedings of the Water Environment Federation ◽

10.2175/193864718825137557 ◽

2018 ◽

Vol 2018 (17) ◽

pp. 440-441

Author(s):

Chris Jepson

Keyword(s):

Peracetic Acid

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Inactivation of Murine Norovirus and MS2 Bacteriophage by Peracetic Acid and Monochloramine in Municipal Secondary Wastewater Effluent

Proceedings of the Water Environment Federation ◽

10.2175/193864717822157955 ◽

2017 ◽

Vol 2017 (9) ◽

pp. 3095-3102

Author(s):

Joseph G Jacangelo ◽

Nathan Dunkin ◽

Shih-Chi Weng ◽

James McQuarrie ◽

Kati Bell ◽

...

Keyword(s):

Peracetic Acid ◽

Wastewater Effluent ◽

Murine Norovirus ◽

Ms2 Bacteriophage

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Inhibition Properties of Arylsulfatase and β-Glucuronidase by Hydrogen Peroxide, Hypochlorite, and Peracetic Acid

ACS Omega ◽

10.1021/acsomega.0c06060 ◽

2021 ◽

Author(s):

Shu-shu Zhong ◽

Jun Zhang ◽

Ze-hua Liu ◽

Zhi Dang ◽

Yu Liu

Keyword(s):

Hydrogen Peroxide ◽

Peracetic Acid

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Influence of peracetic acid-ethanol sterilisation on the biomechanical properties of human meniscus transplants

Journal of Experimental Orthopaedics ◽

10.1186/s40634-021-00336-z ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Volker Eras ◽

Josefine Graffunder ◽

Norus Ahmed ◽

Jan C. Brune

Keyword(s):

Peracetic Acid ◽

Biomechanical Properties ◽

Bone Block ◽

Test Device ◽

Level Of Evidence ◽

Load To Failure ◽

Graft Extrusion ◽

Fresh Frozen ◽

Failure Loads ◽

N Loading

Abstract Purpose Meniscus allograft transplantation (MAT) is a possible treatment for patients suffering with pain after meniscectomy. Here, peracetic acid (PAA) sterilised meniscus transplants were investigated on whether they would provide an adequate alternative to fresh-frozen transplants in their viscoelastic and mechanical properties. Methods In this analysis, 31 menisci donors (26 male and 5 female) were included. The average donor age was 49.87 years, ranging from 32 to 65 years. Menisci of matched pairs of knees underwent chemical sterilisation while counterparts were left fresh-frozen. Stiffness and load to failure were determined via suture retention. Further menisci were analysed while attached to the tibial bone block using a novel test device to mimic physiological load distribution. Meniscus relaxation, stiffness and failure loads were determined. Histology and biphasic properties of the menisci were examined and results were analysed using paired t-tests. Results A novel custom built test device allowed the application of physiological loads for suture retention testing and revealed no significant differences between PAA sterilised (14.85 ± 4.46 N/mm, 50.49 ± 17.01 N) and fresh-frozen (18.26 ± 4.46 N/mm, 59.49 ± 21.07 N) regarding stiffness and failure load, respectively. Furthermore, initial 200 N loading showed significantly higher strain in sterilised menisci (18.87 ± 1.56) compared to fresh frozen (13.81 ± 1.04). Load relaxation experiments demonstrated significantly lower relaxation for sterilised menisci (77.71 ± 1.62) compared to fresh-frozen (89.11 ± 1.00, p-value < 0.0001). Conclusion Peracetic acid sterilised human menisci performed equally to fresh-frozen counterparts in a suture retention test and in physiological failure testing providing an adequate alternative. However, meniscus relaxation, biphasic properties and strain were shown to be significantly different between the groups. A common problem of MAT is graft extrusion or shrinkage, therefore the parameters measured here should be considered and may influence meniscus extrusion after transplantation. Level of evidence n/a (experimental study)

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