"Elastic" fibers in the mesogloea of Pachycerianthus torreyi (Anthozoa)

Sulfonic acid groups were oxidatively generated in the soluble lipid-free lipofuscin component of neuromelanin of human substantia nigra and in lipofuscin of human inferior olive. Exposure of these oxidized, intraneuronal pigments to low pH Alcian blue or aldehyde fuchsin demonstrated an intensity of staining that related to the type of oxidant and the conditions of its use. Utilization of the following oxidants generated increasingly strong staining reactions as signified by the following sequence; periodic acid under mild conditions, bromine in carbon tetrachloride, hydrogen peroxide, periodic acid under drastic conditions, potassium permanganate followed by oxalic acid, hydrogen peroxide followed by bromine in carbon tetrachloride, potassium permanganate followed by metabisulfite or bisulfite, and performic acid. Neither Alcian blue nor aldehyde fuchsin revealed oxidatively generated aldehyde as judged by 1) their failure or near failure to stain inferior olive lipofuscin following mildly applied periodic acid, and 2) the increase in staining intensity, from moderate to strong, displayed by the soluble lipid-free lipofuscin component of neuromelanin and by inferior olive lipofuscin when potassium permanganate was followed by a rinse with metabisulfite or bisulfite in place of one with oxalic acid.

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SOME HISTOCHEMICAL CHARACTERISTICS OF TISSUES IN LARVAE OF CTENICERA DESTRUCTOR (BROWN) (COLEOPTERA, ELATERIDAE), WITH SPECIAL REFERENCE TO CUTANEOUS SENSILLA

Canadian Journal of Zoology ◽

10.1139/z62-068 ◽

1962 ◽

Vol 40 (5) ◽

pp. 733-746 ◽

Cited By ~ 1

Author(s):

R. Y. Zacharuk

Keyword(s):

Special Reference ◽

Potassium Permanganate ◽

Protein Complex ◽

Recurrent Nerve ◽

Efferent System ◽

Sh Groups ◽

Sensory Axons ◽

Aldehyde Fuchsin ◽

Cuticular Structures ◽

Staining Characteristic

The sensory axons from the cutaneous sensilla and some of those in the recurrent nerve stain strongly with S-specific stains. The axons of the efferent system and those from the ocelli lack this staining characteristic. This difference among axons possibly is related to the origin of their precursors in the ontogenetic sequence.Some of the metabolites involved in the synthesis of cuticular structures are demonstrated and discussed. The following sequence in the synthesis of the cuticula is suggested: glycogens → more complex, diastase-fast polysaccharides → chitin → a carbohydrate–protein complex containing SS groups → a complex (procuticle) with potential SH groups → a complex (exocuticle) with bound S and a high content of tyrosine and other phenols. The sequence in the synthesis of the cuticular nerve sheaths appears basically similar. The last step was not evident in tonofibrillae, and the last two steps were not evident in the subcuticular sheaths or axoplasm of reactive axons.The mechanisms of the histochemical reactions are discussed, with particular reference to staining with aldehyde-fuchsin after oxidation with potassium permanganate. This method may serve to differentiate histologically certain afferent from efferent axons in insect nervous systems.

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Ultrastructure and Staining of Developing Elastic Tissue

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065274 ◽

1971 ◽

Vol 29 ◽

pp. 244-245

Author(s):

E. N. Albert

Keyword(s):

Fine Structure ◽

Phosphotungstic Acid ◽

Staining Method ◽

Rat Aorta ◽

Uranyl Acetate ◽

The Other ◽

Elastic Fibers ◽

Elastic Tissue ◽

Lead Citrate ◽

New Born

Silver tetraphenylporphine sulfonate (Ag-TPPS) was synthesized in this laboratory and used as an electron dense stain for elastic tissue (Fig 1). The procedures for the synthesis of tetraphenylporphine sulfonate and the staining method for mature elastic tissue have been described previously.The fine structure of developing elastic tissue was observed in fetal and new born rat aorta using tetraphenylporphine sulfonate, phosphotungstic acid, uranyl acetate and lead citrate. The newly forming elastica consisted of two morphologically distinct components. These were a central amorphous and a peripheral fibrous. The ratio of the central amorphous and the peripheral fibrillar portion changed in favor of the former with increasing age.It was also observed that the staining properties of the two components were entirely different. The peripheral fibrous component stained with uranyl acetate and/or lead citrate while the central amorphous portion demonstrated no affinity for these stains. On the other hand, the central amorphous portion of developing elastic fibers stained vigorously with silver tetraphenylporphine sulfonate, while the fibrillar part did not (compare figs 2, 3, 4). Based upon the above observations it is proposed that developing elastica consists of two components that are morphologically and chemically different.

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A potassium permanganate fixative for membranes in sections

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161175 ◽

1990 ◽

Vol 48 (3) ◽

pp. 722-723

Author(s):

Jindan Song

Keyword(s):

Potassium Permanganate ◽

Cultured Cells ◽

Calf Serum ◽

Trisodium Citrate ◽

Membrane System ◽

Adenocarcinoma Cell Line ◽

Mouse Embryo Fibroblast ◽

African Green Monkey Kidney ◽

Adenocarcinoma Cell ◽

Green Monkey

Potassium permanganate has been used as a fixative for the botanical specimen and membrane system in thin section by Glauert (1975). A new potassium permanganate fixative ( Trisodium citrate 60mM, Potassium chloride 25mM, Magnesium chloride 35mM, and Potassium permanganate 125mM ) for localizing membranous system in whole_mount cultured cells with standard trasmission electron microscopy and phase_contrast microscopy has been developed). Here, we report that using this new potassium permanganate fixative for membranous system in sections.Cultured cells, CV_1 (African green monkey kidney epithelial cells), Balb/c 3T3 ( Mouse embryo fibroblast ) and MCF_7 (Human adenocarcinoma cell line) were used for this study. All cells were grown on 35mm plastic dishes in DME medium containing 5% calf serum at 37 c with 100% humidity and 5% CO2. Using the potassium permanganate fixative to fix the cells for about 7 minutes. After fixation, the cells were dehydrated in a graded series of ethanol.

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Etching of LLDPE and LLDPE/HDPE blends

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100163460 ◽

1996 ◽

Vol 54 ◽

pp. 200-201

Author(s):

M. S. Bischel ◽

J. M. Schultz

Keyword(s):

Potassium Permanganate ◽

Morphological Features ◽

Narrow Fraction ◽

Potassium Permanganate Solution ◽

Commercial Applications ◽

Microscopy Techniques

Despite its rapidly growing use in commercial applications, the morphology of LLDPE and its blends has not been thoroughly studied by microscopy techniques. As part of a study to examine the morphology of a LLDPE narrow fraction and its blends with HDPE via SEM, TEM and AFM, an appropriate etchant is required. However, no satisfactory recipes could be found in the literature. Mirabella used n-heptane, a solvent for LLDPE, as an etchant to reveal certain morphological features in the SEM, including faint banding in spherulites. A 1992 paper by Bassett included a TEM micrograph of an axialite of LLDPE, etched in a potassium permanganate solution, but no details were given.Attempts to use n-heptane, at 60°C, as an etchant were unsuccessful: depending upon thickness, samples swelled and increased in diameter by 5-10% or more within 15 minutes. Attempts to use the standard 3.5% potassium permanganate solution for HDPE were also unsuccessful: the LLDPE was severely overetched. Weaker solutions were also too severe.

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