Anti-parasitic activity of nano Citrullus colocynthis and nano Capparis spinose against Trichomonas vaginalis in vitro

2021 ◽  
Author(s):  
Musafer H. Al-Ardi
Keyword(s):  
Trichomonas Vaginalis ◽  
Citrullus Colocynthis ◽  
Parasitic Activity

scholarly journals Anti-parasitic activity of nano Citrullus colocynthis and nano Capparis spinose against Trichomonas vaginalis in vitro

2021 ◽  
Author(s):  
Musafer Alardi
Keyword(s):  
Growth Rate ◽  
Side Effects ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Trichomonas Vaginalis ◽  
Citrullus Colocynthis ◽  
Capparis Spinosa ◽  
Parasitic Activity ◽  
Animal Tests

Abstract The use of plant extracts and the benefit of their unique properties in treating various pathogens is the return to mother nature, and an attempt to overcome the problems of side effects resulting from the use of chemical drugs and the ability of some pathogens to resist these drugs. Nanotechnology has strengthened the ability of drugs to reach the target and reduced the size and amount of dose needed for treatment.Nano-extracts of Citrullus colocynthis and Capparis spinosa at concentrations of (100,250 and 500) ppm prepared to the treatment Trichomonas vaginalis in vitro at the time (12 , 24, 72)h. Results compared with the use of 0.1% of metronidazole (500 mg).The results showed that the concentrations (100,250, 500) ppm of C. colocynthis had an inhibitory activity for the growth rate (43.77, 69.15, 89.89) at the time (12, 24 and 72) hours, respectively. The inhibitory activity of C. spinosa was (43.18, 67.41, 87.04) at the same time and concentration, compared with metronidazole (43.47, 70.40, 87.04) at the same time. Neither plants showed severe effects in hemolysis.From the results, it can be concluded that either plant can be used as an alternative to metronidazole after completing human and animal tests.

Efficacy of Punica granatum extract on in vitro and in vivo control of Trichomonas vaginalis

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
GT El Sherbini ◽  
KM Ibrahim ◽  
ET El Sherbini ◽  
NM Abdel Hady ◽  
TA Morsy
Keyword(s):  
Trichomonas Vaginalis ◽  
Punica Granatum

Effect of Citrullus colocynthis Medium on in vitro Oocytes Maturation Rate

2020 ◽  
Vol 23 (12) ◽  
Author(s):  
Nabaa A. Al-Nawab ◽  
Ibtesam R.T. Al-Delfi ◽  
Rusul Muzher Hussein ◽  
Sabaa R. Thamer ◽  
Mohammed R. Thamer
Keyword(s):  
Citrullus Colocynthis ◽  
Maturation Rate

Effect of Verbascum thapsus Ethanol Extract on Induction of Apoptosis in Trichomonas vaginalis in vitro

2015 ◽  
Vol 15 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Zohreh Kashan ◽  
Mohsen Arbabi ◽  
Mahdi Delavari ◽  
Hossein Hooshyar ◽  
Mohsen Taghizadeh ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Ethanol Extract ◽  
Verbascum Thapsus ◽  
Induction Of Apoptosis

Anti-parasitic activity of Annona muricata L. leaf ethanolic extract and its fractions against Toxoplasma gondii in vitro and in vivo

2021 ◽  
pp. 114019
Author(s):  
Natália Carnevalli Miranda ◽  
Ester Cristina Borges Araujo ◽  
Allisson Benatti Justino ◽  
Yusmaris Cariaco ◽  
Caroline Martins Mota ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Ethanolic Extract ◽  
Annona Muricata ◽  
Parasitic Activity

scholarly journals Yeast-based automated high-throughput screens to identify anti-parasitic lead compounds

Open Biology ◽  
2013 ◽  
Vol 3 (2) ◽  
pp. 120158 ◽  
Author(s):  
Elizabeth Bilsland ◽  
Andrew Sparkes ◽  
Kevin Williams ◽  
Harry J. Moss ◽  
Michaela de Clare ◽  
...  
Keyword(s):  
Side Effects ◽  
Trypanosoma Brucei ◽  
Fluorescent Proteins ◽  
Screening Method ◽  
Lead Compounds ◽  
Dihydrofolate Reductases ◽  
Parasitic Activity ◽  
High Throughput Screens ◽  
Specific Inhibitors

We have developed a robust, fully automated anti-parasitic drug-screening method that selects compounds specifically targeting parasite enzymes and not their host counterparts, thus allowing the early elimination of compounds with potential side effects. Our yeast system permits multiple parasite targets to be assayed in parallel owing to the strains’ expression of different fluorescent proteins. A strain expressing the human target is included in the multiplexed screen to exclude compounds that do not discriminate between host and parasite enzymes. This form of assay has the advantages of using known targets and not requiring the in vitro culture of parasites. We performed automated screens for inhibitors of parasite dihydrofolate reductases, N -myristoyltransferases and phosphoglycerate kinases, finding specific inhibitors of parasite targets. We found that our ‘hits’ have significant structural similarities to compounds with in vitro anti-parasitic activity, validating our screens and suggesting targets for hits identified in parasite-based assays. Finally, we demonstrate a 60 per cent success rate for our hit compounds in killing or severely inhibiting the growth of Trypanosoma brucei , the causative agent of African sleeping sickness.

scholarly journals CP30, a Cysteine Proteinase Involved inTrichomonas vaginalis Cytoadherence

2000 ◽  
Vol 68 (9) ◽  
pp. 4907-4912 ◽  
Author(s):  
M. Remedios Mendoza-López ◽  
Cecilia Becerril-Garcia ◽  
Loriz V. Fattel-Facenda ◽  
Leticia Avila-Gonzalez ◽  
Martha E. Ruíz-Tachiquín ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Hela Cell ◽  
Trichomonas Vaginalis ◽  
Cysteine Proteinase ◽  
Collagen Iv ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Cell Monolayers ◽  
Thiol Proteinase

ABSTRACT We describe here the participation of a Trichomonas vaginalis 30-kDa proteinase (CP30) with affinity to the HeLa cell surface in attachment of this parasite to host epithelial cells. The CP30 band is a cysteine proteinase because its activity was inhibited by E-64, a thiol proteinase inhibitor. In two-dimensional substrate gel electrophoresis of total extracts of the trichomonad isolate CNCD 147, three spots with proteolytic activity were detected in the 30-kDa region, in the pI range from 4.5 to 5.5. Two of the spots (pI 4.5 and 5.0) bound to the surfaces of fixed HeLa cells corresponding to the CP30 band. The immunoglobulin G fraction of the rabbit anti-CP30 antiserum that recognized a 30-kDa band by Western blotting and immunoprecipitated CP30 specifically inhibited trichomonal cytoadherence to HeLa cell monolayers in a concentration-dependent manner and reacted with CP30 at the parasite surface. CP30 degraded proteins found on the female urogenital tract, including fibronectin, collagen IV, and hemoglobin. Interestingly, CP30 digested fibronectin and collagen IV only at pH levels between 4.5 and 5.0. Moreover, trichomonosis patients whose diagnosis was confirmed by in vitro culture possessed antibody to CP30 in both sera and vaginal washes, and CP30 activity was found in vaginal washes. Our results suggest that surface CP30 is a cysteine proteinase necessary for trichomonal adherence to human epithelial cells.

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