Analysis of molecular variance and population structure in southern Indian finger millet genotypes using three different molecular markers

2016 ◽  
Vol 19 (4) ◽  
pp. 275-283 ◽  
Author(s):  
Host Antony David Rajendran ◽  
Ramakrishnan Muthusamy ◽  
Antony Caesar Stanislaus ◽  
Thirugnanasambantham Krishnaraj ◽  
Sivasankaran Kuppusamy ◽  
...  
Genome ◽  
2003 ◽  
Vol 46 (3) ◽  
pp. 404-410 ◽  
Author(s):  
V Díaz ◽  
E Ferrer

Primers based on conserved motifs of plant resistance genes were used to generate multilocus molecular markers — referred to as resistance gene analog polymorphisms (RGAPs) — in Pinus oocarpa subsp. oocarpa. Ten populations from three regions of Nicaragua were analyzed with 53 RGAPs. The aim of this study was to determine the levels of within- and between-population diversity with this kind of marker, and to compare estimates with previously obtained results based on RAPD and AFLP. All populations showed high levels of diversity. GST values and the analysis of molecular variance (AMOVA) revealed most variation to be within populations, although significant differences between populations and regions were also detected. This pattern of genetic diversity was similar to that obtained for RAPD and AFLP, which suggests that variation at RGAP loci as detected in this work is mostly influence by non-selective forces.Key words: resistance gene analogs, molecular markers, genetic diversity, population structure, Pinus oocarpa.


Plant Disease ◽  
2021 ◽  
Author(s):  
Yeirme Jaimes ◽  
Carolina Gonzalez ◽  
Jairo Rojas ◽  
Jessica Johana Rivera ◽  
Christian Cilas ◽  
...  

The witches’ broom (Moniliophthora perniciosa) is considered as one of the main threats for cacao production and, consequently, for chocolate production worldwide.. In this work, the genetic diversity and population structure of M. perniciosa were analyzed for 59 isolates collected in five departments of Colombia and using 10 microsatellite markers. Analyses revealed 35 multilocus genotypes (MLGs) and clonal populations structure according to linkage disequilibrium analysis. One of the objectives of this study was to determine whether populations were differentiated by geographic origin or T. cacao host genotype. Analysis of molecular variance, Discriminant Analysis of Principal Components (DAPC) and Bruvo genetic distance suggested that the genetic structure was driven by geographic origin and not by T. cacao genotype. The results of this study were consistent with previous findings obtained in other cocoa producing countries. Important insights were discussed regarding the dispersal patterns of the pathogen in Colombia and the genetic change of its populations due to different environmental conditions.


2013 ◽  
Vol 58 (1) ◽  
pp. 279-285 ◽  
Author(s):  
Lars Hedenäs ◽  
Irene Bisang

Abstract The moss Drepanocladus trifarius (F. Weber & D. Mohr) Broth. ex Paris was used as a model species for a first evaluation of whether male and female haplotype patterns based on a combination of the nuclear molecular markers ITS and gpd, and the chloroplast rpl16 are congruent or not. In 23 female and 23 male shoots from specimens sampled in Northern and Central Europe, sex was determined either by the presence of sexual branches or by a recently developed molecular marker. Analysis of molecular variance (AMOVA) showed that a small (6%) and almost significant (p = 0.066) fraction of the variation within the data is due to differences between the genders and that the haplotype diversity is higher among females than among males. No significant differences in haplotype variation were found among geographical regions. The near significant differences between females and males, despite low variation in ITS and rpl16, in this first study ever of potential differences in haplotype variation between genders of dioecious mosses suggests that further investigations of more specimens and more variable molecular markers should be performed. Such studies could potentially reveal patterns of relevance to understanding, for example, immigration routes and to pin-point refugial areas of different significance to the respective genders.


2017 ◽  
Vol 15 (3) ◽  
pp. e0404 ◽  
Author(s):  
Najat El Moutchou ◽  
Ana M. González-Martínez ◽  
Mouad Chentouf ◽  
Khalid Lairini ◽  
Evangelina Rodero

The main goal of this work was to study the genetic diversity of the Northern Morocco goat population through the analysis of 19 microsatellites in 144 animals from 61 herds. To detect a possible population structure, three distinct geographic subpopulations were characterized as a function of climate and environmental influences. Most of the markers were highly polymorphic, and the results revealed considerable genetic variation across the studied loci. A total of 204 alleles were detected, with an average number of 10.7 per locus. The PIC average was 0.728, and four microsatellites showed a significant deviation (p< 0.05) from Hardy-Weinberg Equilibrium. Analysis of molecular variance (AMOVA) indicated that only 0.5% of the variation corresponded to differences among subpopulations, and 99.5% corresponded to differences among individuals. Factorial correspondence analysis showed intense admixtures across the putative subpopulations, and the subdivision related to geographical or environmental adaptation was undetectable. The Northern Morocco goat population presented high genetic diversity and a lack of population structure. The main reason for these findings is the absence of the breed concept (reproductively closed population), resulting in uncontrolled crossbreeding with exotic breeds and other local goats.


Sign in / Sign up

Export Citation Format

Share Document