Sedimentation sequence of a high-temperature silica-rich hot spring: evidence from isothermal evaporation experiments and from petrology and mineralogy of sinters

2021 ◽  
Vol 36 (2) ◽  
Author(s):  
Ze Yan ◽  
Wenli Xu ◽  
Xiaotong Luo ◽  
Yaxian You ◽  
Huaguo Wen
Keyword(s):  
High Temperature ◽  
Hot Spring ◽  
Isothermal Evaporation

scholarly journals Carbon Source Preference in Chemosynthetic Hot Spring Communities

2015 ◽  
Vol 81 (11) ◽  
pp. 3834-3847 ◽  
Author(s):  
Matthew R. Urschel ◽  
Michael D. Kubo ◽  
Tori M. Hoehler ◽  
John W. Peters ◽  
Eric S. Boyd
Keyword(s):  
High Temperature ◽  
National Park ◽  
Hot Springs ◽  
Dissolved Inorganic Carbon ◽  
Hot Spring ◽  
Affinity Constant ◽  
Rrna Gene ◽  
Organic Substrates ◽  
Short Term ◽  
Acetate Assimilation

ABSTRACTRates of dissolved inorganic carbon (DIC), formate, and acetate mineralization and/or assimilation were determined in 13 high-temperature (>73°C) hot springs in Yellowstone National Park (YNP), Wyoming, in order to evaluate the relative importance of these substrates in supporting microbial metabolism. While 9 of the hot spring communities exhibited rates of DIC assimilation that were greater than those of formate and acetate assimilation, 2 exhibited rates of formate and/or acetate assimilation that exceeded those of DIC assimilation. Overall rates of DIC, formate, and acetate mineralization and assimilation were positively correlated with spring pH but showed little correlation with temperature. Communities sampled from hot springs with similar geochemistries generally exhibited similar rates of substrate transformation, as well as similar community compositions, as revealed by 16S rRNA gene-tagged sequencing. Amendment of microcosms with small (micromolar) amounts of formate suppressed DIC assimilation in short-term (<45-min) incubations, despite the presence of native DIC concentrations that exceeded those of added formate by 2 to 3 orders of magnitude. The concentration of added formate required to suppress DIC assimilation was similar to the affinity constant (Km) for formate transformation, as determined by community kinetic assays. These results suggest that dominant chemoautotrophs in high-temperature communities are facultatively autotrophic or mixotrophic, are adapted to fluctuating nutrient availabilities, and are capable of taking advantage of energy-rich organic substrates when they become available.

High-temperature adapted primitiveProtochlamydiafound inAcanthamoebaisolated from a hot spring can grow in immortalized human epithelial HEp-2 cells

2013 ◽  
Vol 16 (2) ◽  
pp. 486-497 ◽  
Author(s):  
Aya Sampo ◽  
Junji Matsuo ◽  
Chikayo Yamane ◽  
Kenji Yagita ◽  
Shinji Nakamura ◽  
...  
Keyword(s):  
High Temperature ◽  
Hot Spring

scholarly journals Distinct microbial composition and functions in an underground high-temperature hot spring at different depths

2019 ◽  
Author(s):  
Shijie Bai ◽  
Xiaotong Peng
Keyword(s):  
High Temperature ◽  
Microbial Communities ◽  
Hot Springs ◽  
Hot Spring ◽  
Hydrogen Oxidation ◽  
Archaeal Community ◽  
Limited Area ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Different Depths

Abstract. The microbial diversity and functions of three high-temperature neutral hot springs water samples at different depths (0 m, 19 m and 58 m) were investigated based on 16S rRNA gene sequencing and a functional gene array (GeoChip 5.0). The results revealed that the bacterial communities were distinct at different depths in the hot springs. Additionally, in response to the depths, bacterial/archaeal community compositions exhibited shifts over the depth profiles. Aquificae, Alpha-proteobacteria, and Deinococcus-Thermus were the dominating phyla at 0 m, 19 m, and 58 m, respectively. Hydrogenobacter, Sphingobium, and Thermus were the most abundant genera at 0 m, 19 m, and 58 m, respectively. The phylum Thaumarchaeota was the most abundant member of the archaeal community in the samples at different hot spring depths. Functional results of the microbial communities indicated that microbial metabolic functions were mainly related to sulfur, nitrogen cycling, and hydrogen oxidation. In summary, our results demonstrated that distinct microbial communities and functions were found at different depths of hot springs in a very limited area. These findings will provide new insights into the deep-subsurface biosphere associated with terrestrial hot springs.

scholarly journals INTERPRETATION OF ALTERATION MINERALS AROUND THE OMA-HARUKU HOT SPRINGS, CENTRAL OF MOLUCCAS

2019 ◽  
Vol 2 (2) ◽  
pp. 090-096
Author(s):  
Helda Andayany ◽  
Josephus Ronny Kelibulin
Keyword(s):  
Particle Size ◽  
High Temperature ◽  
Hot Springs ◽  
Hot Spring ◽  
Geothermal Field ◽  
Petrographic Analysis ◽  
Rock Samples ◽  
Spring Area ◽  
Alteration Minerals ◽  
Xrd Method

Petrographic analysis to rock samples located at Oma-Haruku hot springs was dominated by mudstone. Another alteration mineral types of the rocks in this area are quartz, aragonite/calcite and feldspar minerals. Petrographic analysis was supported by the XRD method. The method indicated that the alteration minerals content was generally dominated by the presence of aragonite/calcite with particle size of 0.05 - 2 mm, the abundance of 55% and the spread evenly. Based on such presence of aragonite/calcite, one can interpret that the origin rock was carbonate. Alteration minerals which were generally dominated by mudstone indicate that the type of hot spring area in Oma-Haruku is a type of high-temperature reservoir, namely > 175 oC. Therefore, the area is  potentially as geothermal field in Central of Moluccas.

scholarly journals Discovery and Characterization of Thermoproteus Spherical Piliferous Virus 1: a Spherical Archaeal Virus Decorated with Unusual Filaments

2020 ◽  
Vol 94 (11) ◽  
Author(s):  
Ross Hartman ◽  
Lieuwe Biewenga ◽  
Jacob Munson-McGee ◽  
Mohammed Refai ◽  
Eric S. Boyd ◽  
...  
Keyword(s):  
High Temperature ◽  
Hot Spring ◽  
Open Reading Frames ◽  
Host Cells ◽  
Gene Content ◽  
Archaeal Virus ◽  
Virus Attachment ◽  
Archaeal Viruses ◽  
Cell Densities ◽  
Filament Protein

ABSTRACT We describe the discovery of an archaeal virus, one that infects archaea, tentatively named Thermoproteus spherical piliferous virus 1 (TSPV1), which was purified from a Thermoproteales host isolated from a hot spring in Yellowstone National Park (USA). TSPV1 packages an 18.65-kb linear double-stranded DNA (dsDNA) genome with 31 open reading frames (ORFs), whose predicted gene products show little homology to proteins with known functions. A comparison of virus particle morphologies and gene content demonstrates that TSPV1 is a new member of the Globuloviridae family of archaeal viruses. However, unlike other Globuloviridae members, TSPV1 has numerous highly unusual filaments decorating its surface, which can extend hundreds of micrometers from the virion. To our knowledge, similar filaments have not been observed in any other archaeal virus. The filaments are remarkably stable, remaining intact across a broad range of temperature and pH values, and they are resistant to chemical denaturation and proteolysis. A major component of the filaments is a glycosylated 35-kDa TSPV1 protein (TSPV1 GP24). The filament protein lacks detectable homology to structurally or functionally characterized proteins. We propose, given the low host cell densities of hot spring environments, that the TSPV1 filaments serve to increase the probability of virus attachment and entry into host cells. IMPORTANCE High-temperature environments have proven to be an important source for the discovery of new archaeal viruses with unusual particle morphologies and gene content. Our isolation of Thermoproteus spherical piliferous virus 1 (TSPV1), with numerous filaments extending from the virion surface, expands our understanding of viral diversity and provides new insight into viral replication in high-temperature environments.

scholarly journals Genomic Insights of “Candidatus Nitrosocaldaceae” Based on Nine New Metagenome-Assembled Genomes, Including “Candidatus Nitrosothermus” Gen Nov. and Two New Species of “Candidatus Nitrosocaldus”

2021 ◽  
Vol 11 ◽  
Author(s):  
Zhen-Hao Luo ◽  
Manik Prabhu Narsing Rao ◽  
Hao Chen ◽  
Zheng-Shuang Hua ◽  
Qi Li ◽  
...  
Keyword(s):  
High Temperature ◽  
Stress Responses ◽  
Hot Springs ◽  
Ammonia Oxidation ◽  
Hot Spring ◽  
Thermal Adaptation ◽  
Amino Acid Identity ◽  
Phylogenomic Analysis ◽  
Adenosylmethionine Decarboxylase ◽  
Almost All

“Candidatus Nitrosocaldaceae” are globally distributed in neutral or slightly alkaline hot springs and geothermally heated soils. Despite their essential role in the nitrogen cycle in high-temperature ecosystems, they remain poorly understood because they have never been isolated in pure culture, and very few genomes are available. In the present study, a metagenomics approach was employed to obtain “Ca. Nitrosocaldaceae” metagenomic-assembled genomes (MAGs) from hot spring samples collected from India and China. Phylogenomic analysis placed these MAGs within “Ca. Nitrosocaldaceae.” Average nucleotide identity and average amino acid identity analysis suggested the new MAGs represent two novel species of “Candidatus Nitrosocaldus” and a novel genus, herein proposed as “Candidatus Nitrosothermus.” Key genes responsible for chemolithotrophic ammonia oxidation and a thaumarchaeal 3HP/4HB cycle were detected in all MAGs. Furthermore, genes coding for urea degradation were only present in “Ca. Nitrosocaldus,” while biosynthesis of the vitamins, biotin, cobalamin, and riboflavin were detected in almost all MAGs. Comparison of “Ca. Nitrosocaldales/Nitrosocaldaceae” with other AOA revealed 526 specific orthogroups. This included genes related to thermal adaptation (cyclic 2,3-diphosphoglycerate, and S-adenosylmethionine decarboxylase), indicating their importance for life at high temperature. In addition, these MAGs acquired genes from members from archaea (Crenarchaeota) and bacteria (Firmicutes), mainly involved in metabolism and stress responses, which might play a role to allow this group to adapt to thermal habitats.

Fluids and hydrothermal alteration assemblages in a Devonian gold-bearing hot-spring system, Rhynie, Scotland

2003 ◽  
Vol 94 (4) ◽  
pp. 309-324 ◽  
Author(s):  
M. Baron ◽  
S. Hillier ◽  
C. M. Rice ◽  
K. Czapnik ◽  
J. Parnell
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Fault Zone ◽  
Hydrothermal Alteration ◽  
Hot Spring ◽  
Medium Temperature ◽  
Low Salinity ◽  
Major Fault ◽  
Spring System ◽  
Gold Bearing

ABSTRACTHydrothermal alteration at Rhynie, Aberdeenshire, Scotland, is concentrated along a fault zone, which juxtaposes surface deposits and the mineralised feeder zone to the Rhynie hotspring system. Mineralisation consists of breccias and veins filled with quartz, chert, calcite, K-feldspar and pyrite. Associated pervasive alteration comprises a high-temperature K-feldsparquartz-illite facies (formed at 250–350°C), a medium-temperature mixed layered illite/smectitequartz-K-feldspar-chlorite-calcite facies (formed at 150–200°C) and a low-temperature mixed layered illite/smectite-chlorite-calcite facies (formed at 100 to +150°C). The fluids responsible for mineralisation were mainly moderate- to high-temperature (Th =91–360°C), low-salinity (<0·2 to 2·9 wt.% NaCl eq.) H2O-NaCl-heated meteoric fluids comparable to modern and ancient hot-spring systems. The migration of these fluids was mainly restricted to a major fault zone bounding the Devonian basin. Fluids responsible for mineralisation, alteration and cementation elsewhere in the basin were low-temperature (Th 57 to 161°C), low- to high-salinity (<0·2 to 18 wt.% NaCl eq.) H2O-NaCl fluids, which resemble basinal brines.

scholarly journals Molecular identification of amylase-producing thermophilic bacteria isolated from Bukit Gadang Hot Spring, West Sumatra, Indonesia

2020 ◽  
Vol 21 (3) ◽  
Author(s):  
Aulia Ardhi ◽  
Arina Nadenggan Sidauruk ◽  
Nabella Suraya ◽  
Nova Wahyu Pratiwi ◽  
Usman Pato ◽  
...  
Keyword(s):  
High Temperature ◽  
Molecular Identification ◽  
Hot Springs ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Amylase Activity ◽  
Hydrolytic Enzymes ◽  
Molecular Technique ◽  
West Sumatra ◽  
Pharmaceutical Industries

Abstract. Ardhi A, Sidauruk AN, Suraya N, Pratiwi NW, Pato U, Saryono. 2020. Molecular identification of amylase-producing thermophilic bacteria isolated from Bukit Gadang Hot Spring, West Sumatra, Indonesia. Biodiversitas 21: 994-1000.  Amylase is one of the hydrolytic enzymes that is widely used in a wide number of industrial processes such as food, fermentation, textile, paper, detergent, and pharmaceutical industries. Amylase produced by thermophilic bacteria may be thermostable, which is very beneficial in several applications requiring high temperature, for example, the process of gelatinization, liquefaction, and saccharification are performed in high temperature involved in the starch processing. In this study, the amylase-producing ability of thermophilic bacteria isolated from Bukit Gadang hot spring, West Sumatra, Indonesia, was checked and followed by molecular identification. Thirteen isolates that were successfully isolated from the hot springs were microscopically and macroscopically characterized, biochemically tested, and determined their amylase enzyme activity both qualitatively and quantitatively. The isolate that performed the best amylase activity was identified using the molecular technique. The DNA sequencing was carried out in 16S rRNA and continued with BLAST search for species identification. The result of molecular identification showed that the isolate with the best amylase activity was identified as Bacillus licheniformis. The optimum amylase production  (231.33 U/ml)  and the best enzyme-specific activity  (101.79 U/mg) were obtained at the incubation time of 36 hours.

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