Chromosome-level genome assembly and annotation of two lineages of the ant Cataglyphis hispanica: steppingstones towards genomic studies of hybridogenesis and thermal adaptation in desert ants

Cataglyphis are thermophilic ants that forage during the day when temperatures are highest and sometimes close to their critical thermal limit. Several Cataglyphis species have evolved unusual reproductive systems such as facultative queen parthenogenesis or social hybridogenesis, which have not yet been investigated in detail at the molecular level. We generated high-quality genome assemblies for two hybridogenetic lineages of the Iberian ant Cataglyphis hispanica using long-read Nanopore sequencing and exploited chromosome conformation capture (3C) sequencing to assemble contigs into 26 and 27 chromosomes, respectively. Males of one lineage were karyotyped to confirm the number of chromosomes inferred from 3C data. We obtained transcriptomic data to assist gene annotation and built custom repeat libraries for each of the two assemblies. Comparative analyses with 19 other published ant genomes were also conducted. These new genomic resources pave the way for exploring the genetic mechanisms underlying the remarkable thermal adaptation and the molecular mechanisms associated with transitions between different genetic systems characteristics of the ant genus Cataglyphis.

Transcriptional Regulation of Metabolic and Cellular Processes in Durum Wheat (Triticum turgidum subsp. durum) in the Face of Temperature Increasing

The Yaqui Valley, Mexico, has been historically considered as an experimental field for semiarid regions worldwide since temperature is an important constraint affecting durum wheat cultivation. Here, we studied the transcriptional and morphometrical response of durum wheat at an increased temperature (+2 °C) for deciphering molecular mechanisms involved in the thermal adaptation by this crop. The morphometrical assay showed a significant decrease in almost all the evaluated traits (shoot/root length, biovolume index, and dry/shoot weight) except in the dry root weight and the root:shoot ratio. At the transcriptional level, 283 differentially expressed genes (DEGs) were obtained (False Discovery Rate (FDR) ≤ 0.05 and |log2 fold change| ≥ 1.3). From these, functional annotation with MapMan4 and a gene ontology (GO) enrichment analysis with GOSeq were carried out to obtain 27 GO terms significantly enriched (overrepresented FDR ≤ 0.05). Overrepresented and functionally annotated genes belonged to ontologies associated with photosynthetic acclimation, respiration, changes in carbon balance, lipid biosynthesis, the regulation of reactive oxygen species, and the acceleration of physiological progression. These findings are the first insight into the regulation of the mechanism influenced by a temperature increase in durum wheat.

A thermo-bio-architectural framework (ThBA) for finding inspiration in nature: Biomimetic energy efficient building design

<p>Design inspired by nature has been known as biomimicry or biomimetic design that is believed to transform human technologies into a sustainable status through translation of biological models, systems, and processes. Considering energy efficiency as one of the aspects of sustainability in the concept of bio-inspired building design, the problem was how to access the solutions best matched to the design problem. Various tools for finding existing knowledge from a different domain are described but as yet there appears to be no tool for allowing building designers to access the efficient ways found in nature of producing energy, using energy, and recycling resources. What the research investigated was to find if it is possible to develop a generalised thermo-bio-architectural (ThBA) framework by use of which architects would be able to improve the energy performance of buildings in a wide range of climates, by following a systematic process that methodically connects design thermal challenges to thermal adaptation principles used in nature. The ThBA was developed by studying biology to find how thermal regulation strategies used by living organisms can be classified and generalised. The proposed ThBA was confirmed and evaluated before it was used for the rest of the research. The biological part of the ThBA was assessed by biological experts within a focus group session. Having the ThBA confirmed, the research also investigated how the heat transfer principles in buildings can be articulated to be linked to the generalised thermal adaptation strategies in nature. For this, a series of case studies were selected and for each an energy simulation was run to analyse its thermal performance and identify its thermal challenges. Then, the ThBA was used to introduce innovative solutions for improving the thermal performance of the case studies with big energy use to reveal unexpected techniques or technologies. This, however, necessitated its reconfiguration so as to be useful for architects. Testing the ThBA for two extreme climates in New Zealand, highlighted the fact that the simple translation of the majority of biological thermal adaptation principles are being used by architects, although for some, the architectural equivalents did not function in exactly in the same way as biological thermoregulation strategies. The differences were seen either in the central thermoregulatory principles or the broader properties within which the key principles fitted. Apart from that, for both architectural and biological thermoregulatory strategies the heat transfer parameter and methods were the same. Given that, in a context where biomimicry is understood as the imitation of complicated thermoregulatory solutions in nature for which innovation is evolutionary achieved, the term biomimetics seems to not have a place in the context of bio-inspired energy efficient design considering the current state of technology. The ThBA, however, suggested a few strategies that might address opportunities for designing a new generation of buildings in the future. This implies that the ThBA is more useful for researchers than architects.</p>

A thermo-bio-architectural framework (ThBA) for finding inspiration in nature: Biomimetic energy efficient building design

<p>Design inspired by nature has been known as biomimicry or biomimetic design that is believed to transform human technologies into a sustainable status through translation of biological models, systems, and processes. Considering energy efficiency as one of the aspects of sustainability in the concept of bio-inspired building design, the problem was how to access the solutions best matched to the design problem. Various tools for finding existing knowledge from a different domain are described but as yet there appears to be no tool for allowing building designers to access the efficient ways found in nature of producing energy, using energy, and recycling resources. What the research investigated was to find if it is possible to develop a generalised thermo-bio-architectural (ThBA) framework by use of which architects would be able to improve the energy performance of buildings in a wide range of climates, by following a systematic process that methodically connects design thermal challenges to thermal adaptation principles used in nature. The ThBA was developed by studying biology to find how thermal regulation strategies used by living organisms can be classified and generalised. The proposed ThBA was confirmed and evaluated before it was used for the rest of the research. The biological part of the ThBA was assessed by biological experts within a focus group session. Having the ThBA confirmed, the research also investigated how the heat transfer principles in buildings can be articulated to be linked to the generalised thermal adaptation strategies in nature. For this, a series of case studies were selected and for each an energy simulation was run to analyse its thermal performance and identify its thermal challenges. Then, the ThBA was used to introduce innovative solutions for improving the thermal performance of the case studies with big energy use to reveal unexpected techniques or technologies. This, however, necessitated its reconfiguration so as to be useful for architects. Testing the ThBA for two extreme climates in New Zealand, highlighted the fact that the simple translation of the majority of biological thermal adaptation principles are being used by architects, although for some, the architectural equivalents did not function in exactly in the same way as biological thermoregulation strategies. The differences were seen either in the central thermoregulatory principles or the broader properties within which the key principles fitted. Apart from that, for both architectural and biological thermoregulatory strategies the heat transfer parameter and methods were the same. Given that, in a context where biomimicry is understood as the imitation of complicated thermoregulatory solutions in nature for which innovation is evolutionary achieved, the term biomimetics seems to not have a place in the context of bio-inspired energy efficient design considering the current state of technology. The ThBA, however, suggested a few strategies that might address opportunities for designing a new generation of buildings in the future. This implies that the ThBA is more useful for researchers than architects.</p>

Genetic and thermal variation influences adaptation to fluctuating temperature in the seed beetle, Callosobruchus maculatus

The impacts of climate change on biological systems are notoriously difficult to measure, and laboratory studies often do not realistically represent natural fluctuations in environmental conditions. To date, most experimental studies of thermal adaptation test populations at constant temperatures, or they make incremental changes to an otherwise constant mean background state. To address this, we examined the long-term effects of stressful fluctuating daily temperature on several key life history traits in two laboratory populations of the seed beetle, Callosobruchus maculatus. These populations were kept for 19 generations at either a constant control temperature, T=29C, or at a fluctuating daily cycle with Tmean=33C, Tmax=40C, and Tmin=26C. The latter being a simple representation of daily temperatures in southern central India in May, where this species originally evolved. We found that beetles that had evolved in stressful environments were smaller in body size when switched to a constant 29C and had far greater reproductive fitness in comparison to beetles from both the constant control and continuously stressful 33C environments. This suggests that beetles raised in environments with stressful fluctuating temperatures were more phenotypically plastic and had greater genetic variability than control treatment beetles and indicates that populations that experience fluctuations in temperature may be better able to respond to short-term changes in environmental conditions.

A newly discovered behavior (‘tail-belting’) among wild rodents in sub zero conditions

Rodents are among the most successful mammals because they have the ability to adapt to a broad range of environmental conditions. Here, we present the first record of a previously unknown thermal adaptation to cold stress that repeatedly occurred in two species of non-commensal rodents (Apodemus flavicollis and Apodemus agrarius). The classic rodent literature implies that rodents prevent heat loss via a broad range of behavioral adaptations including sheltering, sitting on their tails, curling into a ball, or huddling with conspecifics. Here, we have repeatedly observed an undescribed behavior which we refer to as “tail-belting”. This behavior was performed under cold stress, whereby animals lift and curl the tail medially, before resting it on the dorsal, medial rump while feeding or resting. We documented 115 instances of the tail-belting behavior; 38 in Apodemus agrarius, and 77 in Apodemus flavicollis. Thermal imaging data show the tails remained near ambient temperature even when temperatures were below 0 °C. Since the tail-belting occurred only when the temperature dropped below − 6.9 °C (for A. flavicollis) and − 9.5 °C (for A. agrarius), we surmise that frostbite prevention may be the primary reason for this adaptation. It is likely that tail-belting has not previously been documented because free-ranging mice are rarely-recorded in the wild under extreme cold conditions. Given that these animals are so closely-related to laboratory rodents, this knowledge could potentially be relevant to researchers in various disciplines. We conclude by setting several directions for future research in this area.