Effect of Piriformospora indica and Sebacina vermifera on plant growth and essential oil yield in Thymus vulgaris in vitro and in vivo experiments

There is an increasing interest in developing natural methods to replace the current chemicals used for maintaining postharvest quality of citrus fruits. The essential oil antifungal activity of mint (MEO), basil (BEO), and lavender (LEO) acting as the vapor-phases was tested against Penicillium digitatum. The minimum doses with fungistatic and fungicidal effect, in vitro, acting as the vapor-phases, were set up. The minimum fungicidal dose was 300 μL for BEO and 350 μL LEO, while for MEO only minimal dose with fungistatic effect was reached. The IC50 values were calculated and used (v/v) for testing preservation of lemon fruits, in close space enriched in vapor oil. For this purpose, the following two independent in vivo experiments were carried out: experiment 1, inoculated lemons with P. digitatum stored without chemical treatments 7 days, at 22 ± 2 °C, at two concentrations (C1—IC50 equivalent; C2—half of C1); and experiment 2, the non-inoculated lemons kept under the same conditions and concentrations of EO vapor served to evaluate the lemon quality properties. The results showed that antifungal protective effect was provided in the order of LEO-C1 > BEO-C1 > MEO-C1 > BEO-C2 > MEO-C2 > LEO-C2. The quality indicators like weight loss, pH, and firmness were not negatively influenced.

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Elevated atmospheric CO2 decreases oxidative stress and increases essential oil yield in leaves of Thymus vulgaris grown in a mini-FACE system

Environmental and Experimental Botany ◽

10.1016/j.envexpbot.2008.09.001 ◽

2009 ◽

Vol 65 (1) ◽

pp. 99-106 ◽

Cited By ~ 35

Author(s):

E VURRO ◽

R BRUNI ◽

A BIANCHI ◽

L TOPPI

Keyword(s):

Oxidative Stress ◽

Essential Oil ◽

Atmospheric Co2 ◽

Oil Yield ◽

Thymus Vulgaris ◽

Elevated Atmospheric Co2 ◽

Essential Oil Yield

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Effects of Irrigation with Different Sources of Water on Growth, Yield and Essential Oil Compounds in Oregano

Plants ◽

10.3390/plants9111618 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1618

Author(s):

Giuseppe Virga ◽

Leo Sabatino ◽

Mario Licata ◽

Teresa Tuttolomondo ◽

Claudio Leto ◽

...

Keyword(s):

Essential Oil ◽

Plant Growth ◽

Irrigation Water ◽

Growth And Yield ◽

Giant Reed ◽

Oil Yield ◽

Treated Wastewater ◽

Short Term ◽

Different Sources ◽

Essential Oil Yield

Aromatic plants can benefit from the use of treated wastewater to satisfy their water requirements, but the effects on the essential oil yield and quality need an assessment. The aims of this study were to assess the effects of freshwater and treated wastewater obtained from a Sicilian (Italy) pilot-scale horizontal subsurface flow constructed wetland system on plant growth and yield, essential oil yield and composition of oregano (Origanum vulgare ssp. hirtum (Link) Ietswaart) and soil characteristics. The system had a total surface area of 100 m2 and was planted with giant reed and umbrella sedge. An experimental open field of oregano was set up close to the system. Two years and two different sources of irrigation water were tested in a split-plot design for a two-factor experiment. Treated wastewater was characterized by higher values of mineral and organic constituents than freshwater. The results highlight that short-term irrigation with freshwater and treated wastewater, in both years, led to increased plant growth, dry weight and essential oil yield of oregano plants. However, it did not significantly affect the essential oil content and composition in comparison with the control. Furthermore, the year and source of irrigation water did not significantly vary the chemical composition of the soil. Our results suggest that treated wastewater can be considered an alternative to freshwater for the cultivation of oregano due to the fact that it does not greatly influence the yield quality and quantity of this species in the short-term.

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Essential oils in the control of dry bubble disease in white button mushroom

Ciência Rural ◽

10.1590/0103-8478cr20160780 ◽

2017 ◽

Vol 47 (5) ◽

Cited By ~ 2

Author(s):

Tamara Leite dos Santos ◽

Leônidas Leoni Belan ◽

Diego Cunha Zied ◽

Eustáquio Souza Dias ◽

Eduardo Alves

Keyword(s):

Essential Oils ◽

Thymus Vulgaris ◽

Good Growth ◽

Syzygium Aromaticum ◽

Cinnamon Oil ◽

In Vivo Experiments ◽

Dry Bubble ◽

Dry Bubble Disease

ABSTRACT: Lecanicillium fungicola, which causes Dry bubble disease, induces infections and inflicts major losses in champignon production. The control can be managed through measures of hygiene and use of fungicides; however, in Brazil there are no registered products. This study aimed to estimate the influence of various essential oils extracted from Melissa officinalis, Thymus vulgaris, Origanum vulgare, Eucalyptus globulus, Cinnamomum zeylanicum and Syzygium aromaticum on the in vitro development and their uses. Therefore, analysis was performed of the L. fungicola isolates in vitro and the best oils were tested in vivo. Besides, the Agaricus bisporus - L. fungicola interaction was confirmed by scanning electron microscopy (SEM). Cinnamon and clove oils in concentrations of 0.4% and thyme oil of 0.8% were identified as good growth inhibitors of the pathogenic mycelium. Effective inhibition of the conidial germination was seen in all concentrations by cinnamon oil, and by clove and thyme oils only at 0.4% and 0.8%, respectively. When the essential oils were applied post-infestation in the in vivo experiments the incidence of the disease in the mushrooms was much lower. From the SEM it was clear that 19 hours after the inoculation of A. bisporus with L. fungicola, the spores had already completely germinated, revealing the presence of the infection. Therefore, the findings of this study indicated that the oil extracts of cinnamon, clove and thyme are potential and efficient alternatives in the control of dry bubble disease.

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Effect of plant growth regulators and organic manure on some morphological characters, biomass and essential oil yield of dragonhead (Dracocephalum moldavica) / Augalų augimo reguliatorių ir organinių trąšų poveikis darželinės žiomenės (Dracocephalum moldavica) morfologiniams požymiams, biomasei ir eterinio aliejaus išeigai

Botanica Lithuanica ◽

10.1515/botlit-2016-0013 ◽

2016 ◽

Vol 22 (2) ◽

pp. 123-132 ◽

Cited By ~ 1

Author(s):

Yousef Nasiri

Keyword(s):

Essential Oil ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Foliar Application ◽

Block Design ◽

Farmyard Manure ◽

Morphological Characters ◽

Oil Yield ◽

Essential Oil Yield

AbstractTo study the effect of farmyard manure and plant growth regulators on yield and essential oils of dragonhead, an outdoor experiment was conducted in Maragheh, Iran. The experiment was carried out based on randomized complete block design with three replications. Three amounts of farmyard manure (FYM) including 0, 10 and 20 t/ha together with foliar growth regulators including 0, salicylic acid (SA), ascorbic acid (ASc) and SA + ASc were used. The results revealed that application of 10 and 20 t/ha farmyard manure significantly increased plant height and the number of lateral stems. However, application of 20 t/ha led to the increase of the number of the main and flowering stems, biomass and essential oil percentage of dragonhead compared to control. The effect of growth regulators on the number of lateral and flowering stems, and biomass was significant: foliar application of SA and ASc separately or in combination increased the number of lateral stems; however, the number of flowering stems and biomass of dragonhead increased only when both growth regulators were used in combination (SA + ASc). The results also indicated that the highest length of inflorescence (13.57 cm) and essential oil yield (41.83 kg/ha), by 31.4 and 126.3% increment, respectively, were observed under foliar application of 20 t/ha farmyard manure together with SA + ASc application compared to the control.

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IN VITRO AND IN VIVO EFFICIENCY OF FENNEL ESSENTIAL OIL AGAINST TOMATO FUSARIUM WILT AND ITS PROMOTION EFFECT ON PLANT GROWTH

International Journal of Agriculture Environment and Bioresearch ◽

10.35410/ijaeb.2019.4417 ◽

2019 ◽

Vol 4 (4) ◽

pp. 180-199

Author(s):

Fatma Kalleli ◽

Ibtissem Ben Salem ◽

Naïma Boughalleb-M’Hamdi ◽

Mahmoud M’Hamdi

Keyword(s):

Essential Oil ◽

Plant Growth ◽

Fusarium Wilt ◽

Promotion Effect

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Balık Hastalıkları ile Mücadelede Bitki Esansiyel Yağlarının Major Komponentlerinin Önemi Üzerine bir Araştırma

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v6i10.1501-1506.2151 ◽

2018 ◽

Vol 6 (10) ◽

pp. 1501

Author(s):

Azime Küçükgül

Keyword(s):

Phenolic Compounds ◽

Essential Oil ◽

Essential Oils ◽

Thymus Vulgaris ◽

Therapeutic Effects ◽

Alpha Pinene ◽

Phytochemical Components ◽

In Vivo Effects

The presence of different phytochemical components of essential oil such as tannins, alkaloids, terpenoids and phenolic compounds has antibacterial, antifungal, and anti-inflammatory effects. The aim of this study is to investigate importance of the major components of three herb essential oils (Thymus vulgaris L., Centauriumerythraea Rafn. And Foeniculumvulgare Mill) on challenging with fish diseases. The components of essential oils provided from a commercial firm were made GC/MS analyzes. The major component of T. vulgarewas carvacrol called as phenol, 2-methyl-5-(1-methylethyl) with 40%.The others were Linalool L (15.11%) and benzene, methyl(1-methylethyl)- (12.12%).The richest oil in C. erythraea was bicyclo[3.1.1]hept-2-ene, 2,6,6-trimethyl- (34.90%) called as alpha-pinene, followed by heptacosane (19.15%) and dotriacontane (17.72%), respectively.The evaluation of the essential oil of F. vulgarispresented benzene, 1-methoxy-4-(1-propenyl)- with 67.99%, followed by dl-Limonene (16.03%) and benzene, 1-methoxy-4-(2-propenyl)- (6.97%). The therapeutic effects of thyme are due to its high content of phenolic compounds, particularly carvacrol. The most important compounds of F. vulgare essential oil is anethole, fenchone, limonene that has antibacterial, antioxidant, antifungal and anticancer effects. The previous studies show that alpha pinene significantly inhibits many pathogenic Gram-negative bacteria. In aquaculture studies, in vitro and in vivo effects of the dominant compounds of essential oils in our study are consistent with the previous findings.

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