scholarly journals Overview report on the application of double digest selective label (DDSL) bacteria genotyping technique for identification of strains and certification of commercially used bacteria

3 Biotech ◽  
2020 ◽  
Vol 10 (2) ◽  
Author(s):  
V. Terletskiy
Keyword(s):  
Selective Label

scholarly journals Ultra selective label free electrochemical detection of cancer prognostic p53-antibody at DNA functionalized graphene

2019 ◽  
Vol 23 ◽  
pp. 100261 ◽  
Author(s):  
Habibulla Imran ◽  
Palinci Nagarajan Manikandan ◽  
Dhamodharan Prabhu ◽  
Venkataraman Dharuman ◽  
Jeyaraman Jeyakanthan ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Functionalized Graphene ◽  
Label Free ◽  
Selective Label ◽  
P53 Antibody

scholarly journals Development of a NanoMIPs-SPR-Based Sensor for β-Lactoglobulin Detection

Chemosensors ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 94
Author(s):  
Roberta D’Aurelio ◽  
Jon Ashley ◽  
Thomas L. Rodgers ◽  
Linda Trinh ◽  
Jeff Temblay ◽  
...  
Keyword(s):  
Solid Phase ◽  
Cross Contamination ◽  
Label Free ◽  
Phase Synthesis ◽  
Processing Plants ◽  
On Line ◽  
Amine Groups ◽  
Final Optimization ◽  
Β Lactoglobulin ◽  
Selective Label

Food manufacturers are aiming to manage the levels of cross-contamination of allergens within food processing plants and ultimately move away from precautionary labelling. Hence, the need for rapid methods to detect allergens cross-contamination. A sensitive and selective label-free nanoMIPs based sensor was developed and tested for the detection of β-lactoglobulin (BLG). NanoMIPs were synthesized using solid-phase synthesis and appeared as spherical nanoparticles with sizes ranging from 264–294 nm, using dynamic light scattering (DLS). The nanoMIPs were functionalized with amine groups and attached to the surface of the SPR gold chip via amine-coupling protocol. The SPR nanoMIPs-based sensor demonstrated a detection limit of 3 ng mL−1 (211 pM) over a linear range of 1–5000 ng mL−1, with binding affinity of 7.0 × 10−8 M and specificity towards BLG. With further testing and final optimization, the developed nanosensor can be integrated on-line or at-line cleaning-in-place (CIP) wash systems, allowing to effectively monitor milk protein allergens as a rapid, point-of-source methodology.

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