Identification and function prediction of iron-deficiency-responsive microRNAs in citrus leaves

Biological information of protein primary structure is responsible for finding the protein function, extracting features and function of a protein in the biology lab is challenging and time-consuming. Identification of protein function provides essential information for the treatment of various diseases and drug design. Therefore, extracting the protein knowledge from primary structure alone has been a diverse field in the study of bioinformatics data mining and computational biology. This study aimed to function prediction of protein primary structure using the LSTM methods. PRNP(prion protein )most of the nervous system tissues express by prion protein, this is generally to protease-resistant from disease, due to this reasons, the human codon PRNP is most closely associated with Alzheimer disease. The PRNP protein data trained with Hemo sapiens PRNP selection, classification was implemented with network layer perceptron. The learning algorithms are frame by the nervous system. The training results observation indicate that the learning success of prion protein classification leads positively.

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The Structure, Expression, and Function Prediction of DAZAP2, A Down-Regulated Gene in Multiple Myeloma

Genomics Proteomics & Bioinformatics ◽

10.1016/s1672-0229(04)02007-8 ◽

2004 ◽

Vol 2 (1) ◽

pp. 47-54 ◽

Cited By ~ 10

Author(s):

Yiwu Shi ◽

Saiqun Luo ◽

Jianbin Peng ◽

Chenghan Huang ◽

Daren Tan ◽

...

Keyword(s):

Multiple Myeloma ◽

Function Prediction ◽

And Function

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Expression of the two mRNA isoforms of the iron transporter Nrmap2/DMTI in mice and function of the iron responsive element

Biochemical Journal ◽

10.1042/bj3630449 ◽

2002 ◽

Vol 363 (3) ◽

pp. 449-455 ◽

Cited By ~ 45

Author(s):

Dimitri TCHERNITCHKO ◽

Monique BOURGEOIS ◽

Marie-Elise MARTIN ◽

Carole BEAUMONT

Keyword(s):

Alternative Splicing ◽

Iron Deficiency ◽

Iron Status ◽

Mrna Isoforms ◽

Real Time Quantitative Pcr ◽

Iron Responsive Element ◽

Mouse Tissues ◽

Stimulation Of Erythropoiesis ◽

Responsive Element ◽

And Function

Nramp2/DMT1 is a transmembrane proton-coupled Fe2+ transporter. Two different mRNAs are generated by alternative splicing; isoform I contains an iron responsive element (IRE), whereas isoform II does not. They encode two proteins differing at their C-terminal end and by their subcellular localization. IRE-mediated stabilization of isoform I mRNA is thought to stimulate DMT1 expression in response to iron deficiency. We have measured the two mRNAs by real-time quantitative PCR in several mouse tissues, in normal conditions or following injection of phenylhydrazine, a potent haemolytic agent. Isoform I mRNA is expressed in the duodenum and is induced by stimulation of erythropoiesis, whereas the non-IRE isoform is mostly induced in erythropoietic spleen. Surprisingly, both isoforms are highly expressed in the kidney and are not regulated by erythropoiesis. To evaluate the role of the IRE in regulating isoform I mRNA stability, in response to variations in cell iron status, several constructs were made in pCDNA3 with either a normal or a mutated IRE placed at the 3′ end of a stable mRNA. These constructs were transfected into HT29 cells and mRNAs were analysed after growing cells in the presence or absence of exogenous iron. There was no difference in the level of expression of the different messages, suggesting that the IRE does not regulate stability of isoform I mRNA. The half-life of the endogenous IRE-mRNA was also measured following actinomycin D addition in iron- or desferrioxamine-treated cells. Decay of the mRNA was very similar in both conditions. These results suggest that additional transcriptional regulations at the promoter level, or iron-dependent regulation of alternative splicing are likely to participate in the induction of isoform I mRNA by iron deficiency.

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Classification and Function Prediction of Proteins Using Diagnostic Amino Acid Patterns

Computer Analysis of Sequence Data ◽

10.1385/0-89603-276-0:295 ◽

1994 ◽

pp. 295-305

Author(s):

Aiala Reizer ◽

Milton H. Saier ◽

Jonathan Reizer

Keyword(s):

Amino Acid ◽

Function Prediction ◽

And Function

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THE GASTROINTESTINAL EFFECTS OF IRON-DEFICIENCY ANEMIA

PEDIATRICS ◽

10.1542/peds.33.1.83 ◽

1964 ◽

Vol 33 (1) ◽

pp. 83-99

Author(s):

J. L. Naiman ◽

F. A. Oski ◽

L. K. Diamond ◽

G. F. Vawter ◽

H. Shwachman

Keyword(s):

Iron Deficiency ◽

Iron Deficiency Anemia ◽

Deficiency Anemia ◽

Control Group ◽

Poor Correlation ◽

Iron Treatment ◽

High Incidence ◽

Before And After ◽

And Function ◽

Duodenal Biopsies

A group of 14 infants and children with nutritional iron-deficiency anemia were studied with respect to gastrointestinal function and structure both before and after iron treatment. A high incidence of abnormalities was found, consisting of gastric achlorhydria, impaired absorption of xylose and vitamin A, and steatorrhea. Duodenal biopsies revealed varying degrees of chronic duodenitis and mucosal atrophy. There was poor correlation between structure and function. Following treatment with oral iron most of the abnormalities reverted to normal. A control group of 8 children with long-standing anemias not due to iron deficiency were subjected to similar studies, most of which yielded normal results. These observations indicate a diffuse and reversible enteropathy in children as a result of iron deficiency. The pathogenesis and practical implications are discussed.

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