Development of polyclonal antibodies using bacterially expressed recombinant coat protein for the detection of Onion yellow dwarf virus (OYDV) and identification of virus free onion genotypes

3 Biotech ◽  
2021 ◽  
Vol 11 (8) ◽  
Author(s):  
Rakesh Kumar ◽  
Rajendra Prasad Pant ◽  
Sonia Kapoor ◽  
Anil Khar ◽  
Virendra Kumar Baranwal
Keyword(s):  
Coat Protein ◽  
Polyclonal Antibodies ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Yellow Dwarf Virus ◽  
Recombinant Coat Protein

Genetic diversity and evidence of recombination in the coat protein gene of Onion yellow dwarf virus

2015 ◽  
Vol 142 (2) ◽  
pp. 377-387
Author(s):  
Maria Bereda ◽  
Elżbieta Paduch-Cichal ◽  
Elżbieta Kalinowska ◽  
Marek Stefan Szyndel
Keyword(s):  
Genetic Diversity ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Yellow Dwarf Virus

scholarly journals Double Infection of Onion yellow dwarf virus and Shallot latent virus in Garlic from Several Regions in Indonesia

2021 ◽  
Vol 25 (1) ◽  
pp. 40
Author(s):  
Nurenik Nurenik ◽  
Sedyo Hartono ◽  
Sri Sulandari ◽  
Susamto Somowiyarjo ◽  
Argawi Kandito
Keyword(s):  
Early Detection ◽  
Latent Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Rt Pcr ◽  
Double Infection ◽  
Specific Primers ◽  
Shallot Latent Virus ◽  
Yellow Dwarf Virus

Viruses have been a problem on garlic cultivations in various countries. There are several viruses reported infecting garlic. Genera Potyvirus and Carlavirus are the most common viruses found infecting garlic. Mixed infection on garlic is often designated as a “garlic viral complex”. These viruses can be transmitted through imported garlic seeds. Therefore, it is necessary to conduct early detection of garlic seeds to prevent the epidemic of these viruses. This study aimed to detect Onion yellow dwarf virus (OYDV) and Shallot latent virus (SLV) on garlic. Garlic samples were obtained from Enrekang, Magelang, Temanggung, Tawangmangu, and Yogyakarta. Total RNA was extracted from the samples and subsequently used for RT-PCR using two pairs of specific primers SLV-F/SLV-R and OYDV-F/OYDV-R. Primary pair SLV-F/SLV-R in amplicons sized 276 bp, while OYDV-F/OYDV-R in amplicons sized 112 bp. RT-PCR results showed that OYDV was found in all samples tested in this study. Meanwhile, double infections (OYDV and SLV) were found in eight out of ten samples tested. These results indicated that double infections on garlic were common in Indonesia.

Onion yellow dwarf virus. [Distribution map].

2018 ◽  
Author(s):  
Keyword(s):  
New York ◽  
Far East ◽  
Uttar Pradesh ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Distribution Map ◽  
Yellow Dwarf Virus ◽  
Virus Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Onion yellow dwarf virus. Potyviridae: Potyvirus. Hosts: onion (Allium cepa) and garlic (Allium sativum). Information is given on the geographical distribution in Europe (Austria, Croatia, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Italy, mainland Italy, Sicily, Lithuania, Moldova, Netherlands, Poland, Portugal, Romania, Russia, Far East, Siberia, Serbia, Slovenia, Spain, UK, England and Wales and Ukraine), Asia (China, Henan, Hubei, Hunan, Jiangsu, Shandong, Yunnan, Zhejiang, India, Delhi, Gujarat, Haryana, Madhya Pradesh, Maharashtra, Indian Punjab, Rajasthan, Uttar Pradesh, Indonesia, Java, Iran, Israel, Japan, Pakistan, Taiwan, Turkey, Vietnam and Yemen), Africa (Egypt, Morocco, Nigeria and Sudan), North America (Canada, British Columbia, Nova Scotia, Ontario, Mexico, USA, California, Colorado, Florida, Idaho, Iowa, Minnesota, Nevada, New York, Oregon, Texas, Vermont, Washington and West Virginia), Central America and Caribbean (Cuba), South America (Argentina, Brazil, Bahia, Goias, Minas Gerais, Parana, Rio Grande do Sul, Santa Catarina, Sao Paulo, Chile, Ecuador and Urugay) and Oceania (Australia and New Zealand).

RESISTANCE TO ONION YELLOW DWARF VIRUS AND LEEK YELLOW STRIPE VIRUS FOUND IN A FERTILE GARLIC CLONE

2001 ◽  
pp. 243-246 ◽  
Author(s):  
H. Lot ◽  
V. Chovelon ◽  
S. Souche ◽  
B. Delecolle ◽  
T. Etoh ◽  
...  
Keyword(s):  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Leek Yellow Stripe Virus ◽  
Yellow Dwarf Virus ◽  
Yellow Stripe

scholarly journals First report of onion yellow dwarf virus in Ukraine

2019 ◽  
Vol 101 (4) ◽  
pp. 1283-1283
Author(s):  
Halyna Snihur ◽  
Tetiana Shevchenko ◽  
Khrystyna Sherevera ◽  
Irena Budzanivska ◽  
Oleksiy Shevchenko
Keyword(s):  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
First Report ◽  
Yellow Dwarf Virus

Cloning and Phylogenetic Analysis of Coat Protein of Barley yellow dwarf virus Isolates from Different Regions of Pakistan

2011 ◽  
Vol 160 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Nadir Naveed Siddiqui ◽  
Muhammad Ilyas ◽  
Shahid Mansoor ◽  
Abid Azhar ◽  
Muhammad Saeed
Keyword(s):  
Phylogenetic Analysis ◽  
Coat Protein ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus ◽  
Virus Isolates
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