High-solid enzymatic hydrolysis of sugarcane bagasse and ethanol production in repeated batch process using column reactors

Abstract Biological pretreatment was investigated to increase ethanol production from lignocellulosic biomass, like sugarcane bagasse. Enzyme secretion, changes in substrate composition, enzymatic hydrolysis and ethanol yield after pretreatment by different basidiomycetes were evaluated. Analysis by Fourier transform infrared spectroscopy showed that P. pulmonarius PS2001 and T. villosa 82I6 promoted more extensive selective modifications in the lignin content. Glucose release during enzymatic hydrolysis of samples pretreated with P. pulmonarius PS2001 for 35, 42 and 49 days and with T. villosa 82I6 for 21, 28 and 49 days were higher than the control (48.5±2.38 mg/g), i.e. 68.4 ±0.7, 76.3 ±1.6 and 76.5±2.1 mg/g and 70.9±8.3, 77.8±5.8 and 77.6±4.2 mg/g, respectively. During the fermentation of hydrolysates of samples pretreated with T. villosa 82I6 for 28 and 49 days, a maximum ethanol yield of 19.1±2.8 and 20.2±0.5 mg/g, respectively, was achieved. A positive effect of biological pretreatment on hydrolysis and fermentation was demonstrated.

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The Ways of Factors Influencing High-Solid Enzymatic Hydrolysis of Sugarcane Bagasse Treated by Liquid Hot Water

ChemistrySelect ◽

10.1002/slct.201701353 ◽

2017 ◽

Vol 2 (22) ◽

pp. 6240-6244 ◽

Cited By ~ 2

Author(s):

Wen Wang ◽

Qiong Wang ◽

Xuesong Tan ◽

Wei Qi ◽

Qiang Yu ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

Hot Water ◽

High Solid ◽

Factors Influencing ◽

Liquid Hot Water ◽

Hydrolysis Of

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A novel recyclable furoic acid-assisted pretreatment for sugarcane bagasse biorefinery in co-production of xylooligosaccharides and glucose

Biotechnology for Biofuels ◽

10.1186/s13068-021-01884-3 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Lin Dai ◽

Tian Huang ◽

Kankan Jiang ◽

Xin Zhou ◽

Yong Xu

Keyword(s):

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

Production Cost ◽

Solid Loading ◽

Fed Batch ◽

Acid Pretreatment ◽

Furoic Acid ◽

Cooling Crystallization ◽

Mineral Acids ◽

Hydrolysis Of

Abstract Background Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis; and organic acids pretreatment with dual benefic for generating xylooligosaccharides and boosting enzymatic hydrolysis has been widely used in adding values to lignocellulose materials. In this work, furoic acid, a novel recyclable organic acid as catalyst, was employed to pretreat sugarcane bagasse to recover the xylooligosaccharides fraction from hemicellulose and boost the subsequent cellulose saccharification. Results The FA-assisted hydrolysis of sugarcane bagasse using 3% furoic acid at 170 °C for 15 min resulted in the highest xylooligosaccharides yield of 45.6%; subsequently, 83.1 g/L of glucose was harvested by a fed-batch operation with a solid loading of 15%. Overall, a total of 120 g of xylooligosaccharides and 335 g glucose could be collected from 1000 g sugarcane bagasse starting from the furoic acid pretreatment. Furthermore, furoic acid can be easily recovered by cooling crystallization. Conclusion This work put forward a novel furoic acid pretreatment method to convert sugarcane bagasse into xylooligosaccharides and glucose, which provides a strategy that the sugar and nutraceutical industries can be used to reduce the production cost. The developed process showed that the yields of xylooligosaccharides and byproducts were controllable by shortening the reaction time; meanwhile, the recyclability of furoic acid also can potentially reduce the pretreatment cost and potentially replace the traditional mineral acids pretreatment.

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