First Report of Peanut Mottle Virus Infecting Peanut in Northeast China

Plants in the genus Prunus of the family Rosaceae are important fruit and ornamental trees in China. In June of 2007, sweet cherry (Prunus avium) trees with mottling and mosaic symptoms were observed in a private garden near Kunming, Yunnan Province. Twenty-four samples, six each from sweet cherry, sour cherry (P. cerasus), flowering cherry (P. serrulata), and peach (P. persica) were collected from trees in private and community gardens in the area. The peach and sour and flowering cherry trees did not show any symptoms. Total nucleic acids were extracted using a cetyltrimethylammoniumbromide (CTAB) extraction method, and the extracts were tested for the following eight viruses by reverse transcription (RT)-PCR: American plum line pattern virus, Apple chlorotic leaf spot virus, Cherry green ring mottle virus, Cherry necrotic rusty mottle virus, Cherry virus A (CVA), Little cherry virus 1, Prune dwarf virus, and Prunus necrotic ringspot virus. Only CVA was detected in two symptomatic sweet cherry trees by RT-PCR with forward (5′-GTGGCATTCAACTAGCACCTAT-3′) and reverse (5′-TCAGCTGCCTCAGCTTGGC-3′) primers specific to an 873-bp fragment of the CVA replicase gene (2). The CVA infection of the two trees was confirmed by RT-PCR using primers CVA-7097U and CVA-7383L that amplified a 287-bp fragment from the 3′-untranslated region (UTR) of the virus (1). Amplicons from both amplifications were cloned and sequenced. Analysis of the predicted amino acid sequences of the 873-bp fragments (GenBank Accession Nos. EU862278 and EU862279) showed that they were 98% identical with each other and 97 to 98% with the type isolate of CVA from Germany (GenBank Accession No. NC_003689). The 286-bp sequences of the 3′-UTR (GenBank Accession Nos. FJ608982 and FJ608983) were 93% identical with each other and 93 to 98% with the type isolate. The sequence indicated that the three isolates were very similar and should be considered to be the same strain. CVA is a member of the genus Capillovirus in the family Flexiviridae and has been previously reported in Europe, North America, and Japan. The contribution of CVA to the symptoms observed and its distribution in China remain to be evaluated. To our knowledge, this is the first report of CVA in sweet cherry in China. References: (1) M. Isogai et al. J. Gen. Plant Pathol. 70:288. (2) W. Jelkmann. J. Gen. Virol. 76:2015, 1995.

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First Report on Carnation vein mottle virus in Dianthus barbatus in Korea

The Plant Pathology Journal ◽

10.5423/ppj.2004.20.3.224 ◽

2004 ◽

Vol 20 (3) ◽

pp. 224-228

Author(s):

B.N. Chung ◽

B.D. Kim ◽

G.S. Choi ◽

J.S. Kim

Keyword(s):

Mottle Virus ◽

First Report ◽

Dianthus Barbatus

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Updating the Quarantine Status of Prunus Infecting Viruses in Australia

Viruses ◽

10.3390/v12020246 ◽

2020 ◽

Vol 12 (2) ◽

pp. 246 ◽

Cited By ~ 1

Author(s):

Wycliff M. Kinoti ◽

Narelle Nancarrow ◽

Alison Dann ◽

Brendan C. Rodoni ◽

Fiona E. Constable

Keyword(s):

Polymerase Chain Reaction ◽

High Throughput Sequencing ◽

Mottle Virus ◽

Rt Pcr ◽

Chain Reaction ◽

Virus Family ◽

First Report ◽

Hop Stunt Viroid ◽

Polymerase Chain ◽

Stem Pitting

One hundred Prunus trees, including almond (P. dulcis), apricot (P. armeniaca), nectarine (P. persica var. nucipersica), peach (P. persica), plum (P. domestica), purple leaf plum (P. cerasifera) and sweet cherry (P. avium), were selected from growing regions Australia-wide and tested for the presence of 34 viruses and three viroids using species-specific reverse transcription-polymerase chain reaction (RT-PCR) or polymerase chain reaction (PCR) tests. In addition, the samples were tested using some virus family or genus-based RT-PCR tests. The following viruses were detected: Apple chlorotic leaf spot virus (ACLSV) (13/100), Apple mosaic virus (ApMV) (1/100), Cherry green ring mottle virus (CGRMV) (4/100), Cherry necrotic rusty mottle virus (CNRMV) (2/100), Cherry virus A (CVA) (14/100), Little cherry virus 2 (LChV2) (3/100), Plum bark necrosis stem pitting associated virus (PBNSPaV) (4/100), Prune dwarf virus (PDV) (3/100), Prunus necrotic ringspot virus (PNRSV) (52/100), Hop stunt viroid (HSVd) (9/100) and Peach latent mosaic viroid (PLMVd) (6/100). The results showed that PNRSV is widespread in Prunus trees in Australia. Metagenomic high-throughput sequencing (HTS) and bioinformatics analysis were used to characterise the genomes of some viruses that were detected by RT-PCR tests and Apricot latent virus (ApLV), Apricot vein clearing associated virus (AVCaV), Asian Prunus Virus 2 (APV2) and Nectarine stem pitting-associated virus (NSPaV) were also detected. This is the first report of ApLV, APV2, CGRMV, CNRNV, LChV1, LChV2, NSPaV and PBNSPaV occurring in Australia. It is also the first report of ASGV infecting Prunus species in Australia, although it is known to infect other plant species including pome fruit and citrus.

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First Report of Peanut mottle virus in Forage Peanut (Arachis pintoi) in the United States

Plant Health Progress ◽

10.1094/php-12-17-0076-br ◽

2018 ◽

Vol 19 (1) ◽

pp. 13-14

Author(s):

K. K. Dey ◽

L. Hassell ◽

C. Li ◽

M. Elliott ◽

X. Sun

Keyword(s):

United States ◽

The United States ◽

Mottle Virus ◽

Arachis Glabrata ◽

First Report ◽

Arachis Pintoi ◽

Reverse Transcription Pcr ◽

The World ◽

Pcr Products ◽

The Many

Arachis pintoi is one of the many perennial peanuts grown in many tropical and subtropical countries around the world. Although Peanut mottle virus (PeMoV) was reported in Arachis glabrata from Georgia in 2007, there are no reports of PeMoV infecting A. pintoi in the United States. In June 2017, samples of A. pintoi that originated from Hardee County, FL, plants showed a variety of symptoms ranging from yellowing to dark islands, green vein banding, and mild mottling. They tested positive initially with broad-spectrum lateral flow antibody immunoassay and later were confirmed by sequencing the reverse-transcription PCR products. Detection of PeMoV in A. pintoi is significant because it is transmitted by aphids in a nonpersistent manner and is seed-borne in A. hypogea. It is not known if PeMoV is seed-borne in A. pintoi. However, A. pintoi is commonly vegetatively propagated using stolon cuttings. It is possible that PeMoV can spread to A. pintoi in Florida by all these means, making maintenance of virus-free propagation stock plants important. To our knowledge, this is the first report of PeMoV in A. pintoi the United States.

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First Report of Pepper mild mottle virus in Jalapeno Pepper in Georgia

Plant Health Progress ◽

10.1094/php-2003-1223-01-hn ◽

2003 ◽

Vol 4 (1) ◽

pp. 26 ◽

Cited By ~ 3

Author(s):

Natalia Martínez-Ochoa ◽

David B. Langston ◽

Stephen W. Mullis ◽

James T. Flanders

Keyword(s):

Mottle Virus ◽

Pepper Mild Mottle Virus ◽

First Report ◽

Entire Field ◽

Leaf Tissues

In October of 2002, an entire field of jalapeno pepper in Grady County, Georgia was affected by a virus-like disease. All fruit and leaf tissues and seed samples tested positive for Pepper mild mottle virus (PMMoV). Transmission through both seed and sap inoculation was confirmed under greenhouse conditions. This is the first report of seed-transmitted PMMoV affecting jalapeno peppers in Georgia. Accepted for publication 3 November 2003. Published 23 December 2003.

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