Comparative study of cilnidipine loaded PLGA nanoparticles: process optimization by DoE, physico-chemical characterization and in vivo evaluation

This study’s aim was to evaluate the biocompatibility and bioabsorption of a new membrane for guided bone regeneration (polylactic-co-glycolic acid associated with hydroxyapatite and β-tricalcium phosphate) with three thicknesses (200, 500, and 700 µm) implanted in mice subcutaneously. Scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and the quantification of carbon, hydrogen and nitrogen were used to characterize the physico-chemical properties. One hundred Balb-C mice were divided into 5 experimental groups: Group 1—Sham (without implantation); Group 2—200 μm; Group 3—500 μm; Group 4—700 μm; and Group 5—Pratix®. Each group was subdivided into four experimental periods (7, 30, 60 and 90 days). Samples were collected and processed for histological and histomorphometrical evaluation. The membranes showed no moderate or severe tissue reactions during the experimental periods studied. The 500-μm membrane showed no tissue reaction during any experimental period. The 200-μm membrane began to exhibit fragmentation after 30 days, while the 500-μm and 700-µm membranes began fragmentation at 90 days. All membranes studied were biocompatible and the 500 µm membrane showed the best results for absorption and tissue reaction, indicating its potential for clinical guided bone regeneration.

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Achieving successful delivery of oligonucleotides — From physico-chemical characterization to in vivo evaluation

Biotechnology Advances ◽

10.1016/j.biotechadv.2015.04.008 ◽

2015 ◽

Vol 33 (6) ◽

pp. 1294-1309 ◽

Cited By ~ 24

Author(s):

Anna Scomparin ◽

Dina Polyak ◽

Adva Krivitsky ◽

Ronit Satchi-Fainaro

Keyword(s):

Chemical Characterization ◽

In Vivo Evaluation ◽

Physico Chemical

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Physico-Chemical Characterization and In Vivo Evaluation of Indomethacin Ethyl Ester-Loaded Nanocapsules by PCS, TEM, SAXS, Interfacial Alkaline Hydrolysis and Antiedematogenic Activity

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2006.417 ◽

2006 ◽

Vol 6 (9) ◽

pp. 3154-3162 ◽

Cited By ~ 27

Author(s):

Letícia Cruz ◽

Scheila R. Schaffazick ◽

Teresa Dalla Costa ◽

Leonardo U. Soares ◽

Graziela Mezzalira ◽

...

Keyword(s):

Ethyl Ester ◽

Alkaline Hydrolysis ◽

Polymer Concentration ◽

Chemical Characterization ◽

Drug Carriers ◽

Spherical Particles ◽

In Vivo Evaluation ◽

Physico Chemical ◽

Biexponential Model

Nanocapsules are vesicular drug carriers constituted of an oil core, a polymeric wall, and surfactants. A general understanding about the influence of the polymeric wall of nanocapsules on the release profiles of drugs is not known. So, this work was devoted to characterize formulations prepared without polymer or containing it at different concentrations. The indomethacin ethyl ester was used as model and the strategy was based on its interfacial alkaline hydrolysis simulating a sink condition for the release. The antiedematogenic activity in rats for ester-loaded-nanocarriers was also evaluated. The nanocapsules (NC) and nanoemulsion (NE) presented particle sizes below 300 nm, polydispersity lower than 1.2 and pH around 5. SAXS analyses showed that the sorbitan monostearate is dissolved in the oil and the polymer presents regions of crystallinity independently on the PCL concentration. TEM analyses showed droplets (NE) and spherical particles (NC). The time for the total disappearance of the ester varied from 12 h to 24 h depending on the polymer concentration. The biexponential model showed that the indomethacin ester was essentially entrapped within the nanocarriers in an extension of 85 to 95%. The half-lives varied from 147 to 289 min for the sustained phases and from 3 to 6 min for the burst phases. The ester-loaded-NC showed significant antiedematogenic activity, while the ester-loaded-NE did not inhibit the carrageenin-induced paw edema. The nanocapsules promoted the absorption of the indomethacin ethyl ester and the presence of the polymer is important to achieve the pharmacological effect.

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