Effect of tocopherol on the properties of Pluronic F127 microemulsions: Physico-chemical characterization and in vivo toxicity

2019 ◽  
Vol 277 ◽  
pp. 624-630 ◽  
Author(s):  
Addas Rahdar ◽  
Pablo Taboada ◽  
Mohammad Reza Hajinezhad ◽  
Mahmood Barani ◽  
Hamid Beyzaei
Keyword(s):  
Chemical Characterization ◽  
Pluronic F127 ◽  
In Vivo Toxicity ◽  
Physico Chemical

Metal chelate grafting at the surface of mesoporous silica nanoparticles (MSNs): physico-chemical and biomedical imaging assessment

2015 ◽  
Vol 3 (5) ◽  
pp. 748-758 ◽  
Author(s):  
Myriam Laprise-Pelletier ◽  
Meryem Bouchoucha ◽  
Jean Lagueux ◽  
Pascale Chevallier ◽  
Roger Lecomte ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Biomedical Imaging ◽  
Mesoporous Silica Nanoparticles ◽  
Metal Chelate ◽  
Chemical Characterization ◽  
Physico Chemical

A physico-chemical characterization and dual in vivo imaging (PET/MRI) of MSNs functionalized with DTPA and labeled with Gd3+ and 64Cu2+.

scholarly journals In Vitro Physico-Chemical Characterization and Standardized In Vivo Evaluation of Biocompatibility of a New Synthetic Membrane for Guided Bone Regeneration

Materials ◽  
2019 ◽  
Vol 12 (7) ◽  
pp. 1186
Author(s):  
Lívia da Costa Pereira ◽  
Carlos Fernando de Almeida Barros Mourão ◽  
Adriana Terezinha Neves Novellino Alves ◽  
Rodrigo Figueiredo de Brito Resende ◽  
Marcelo José Pinheiro Guedes de Uzeda ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Chemical Properties ◽  
Chemical Characterization ◽  
Guided Bone Regeneration ◽  
Tissue Reaction ◽  
In Vivo Evaluation ◽  
Physico Chemical ◽  
Tissue Reactions

This study’s aim was to evaluate the biocompatibility and bioabsorption of a new membrane for guided bone regeneration (polylactic-co-glycolic acid associated with hydroxyapatite and β-tricalcium phosphate) with three thicknesses (200, 500, and 700 µm) implanted in mice subcutaneously. Scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and the quantification of carbon, hydrogen and nitrogen were used to characterize the physico-chemical properties. One hundred Balb-C mice were divided into 5 experimental groups: Group 1—Sham (without implantation); Group 2—200 μm; Group 3—500 μm; Group 4—700 μm; and Group 5—Pratix®. Each group was subdivided into four experimental periods (7, 30, 60 and 90 days). Samples were collected and processed for histological and histomorphometrical evaluation. The membranes showed no moderate or severe tissue reactions during the experimental periods studied. The 500-μm membrane showed no tissue reaction during any experimental period. The 200-μm membrane began to exhibit fragmentation after 30 days, while the 500-μm and 700-µm membranes began fragmentation at 90 days. All membranes studied were biocompatible and the 500 µm membrane showed the best results for absorption and tissue reaction, indicating its potential for clinical guided bone regeneration.

scholarly journals Optimization of Innovative Three-Dimensionally-Structured Hybrid Vesicles to Improve the Cutaneous Delivery of Clotrimazole for the Treatment of Topical Candidiasis

Pharmaceutics ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 263 ◽  
Author(s):  
Maria Letizia Manca ◽  
Iris Usach ◽  
José Esteban Peris ◽  
Antonella Ibba ◽  
Germano Orrù ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Chemical Properties ◽  
Chemical Characterization ◽  
Yeast Cells ◽  
Unilamellar Vesicles ◽  
Transmission Electron ◽  
Physico Chemical

New three-dimensionally-structured hybrid phospholipid vesicles, able to load clotrimazole in a high amount (10 mg/mL), were obtained for the first time in this work by significantly reducing the amount of water (≤10%), which was replaced with a mixture of glycerol and ethanol (≈90%). A pre-formulation study was carried out to evaluate the effect of both the composition of the hydrating medium and the concentration of the phospholipid on the physico-chemical properties of hybrid vesicles. Four different three-dimensionally-structured hybrid vesicles were selected as ideal systems for the topical application of clotrimazole. An extensive physico-chemical characterization performed using transmission electron microscopy (TEM), cryogenic transmission electron microscopy (cryo-TEM), 31P-NMR, and small-angle X-ray scattering (SAXS) displayed the formation of small, multi-, and unilamellar vesicles very close to each other, and was capable of forming a three-dimensional network, which stabilized the dispersion. Additionally, the dilution of the dispersion with water reduced the interactions between vesicles, leading to the formation of single unilamellar vesicles. The evaluation of the in vitro percutaneous delivery of clotrimazole showed an improved drug deposition in the skin strata provided by the three-dimensionally-structured vesicles with respect to the commercial cream (Canesten®) used as a reference. Hybrid vesicles were highly biocompatible and showed a significant antifungal activity in vitro, greater than the commercial cream Canesten®. The antimycotic efficacy of formulations was confirmed by the reduced proliferation of the yeast cells at the site of infection in vivo. In light of these results, clotrimazole-loaded, three-dimensionally-structured hybrid vesicles appear to be one of the most innovative and promising formulations for the treatment of candidiasis infections.

Achieving successful delivery of oligonucleotides — From physico-chemical characterization to in vivo evaluation

2015 ◽  
Vol 33 (6) ◽  
pp. 1294-1309 ◽  
Author(s):  
Anna Scomparin ◽  
Dina Polyak ◽  
Adva Krivitsky ◽  
Ronit Satchi-Fainaro
Keyword(s):  
Chemical Characterization ◽  
In Vivo Evaluation ◽  
Physico Chemical

Physico-Chemical Characterization and In Vivo Evaluation of Indomethacin Ethyl Ester-Loaded Nanocapsules by PCS, TEM, SAXS, Interfacial Alkaline Hydrolysis and Antiedematogenic Activity

2006 ◽  
Vol 6 (9) ◽  
pp. 3154-3162 ◽  
Author(s):  
Letícia Cruz ◽  
Scheila R. Schaffazick ◽  
Teresa Dalla Costa ◽  
Leonardo U. Soares ◽  
Graziela Mezzalira ◽  
...  
Keyword(s):  
Ethyl Ester ◽  
Alkaline Hydrolysis ◽  
Polymer Concentration ◽  
Chemical Characterization ◽  
Drug Carriers ◽  
Spherical Particles ◽  
In Vivo Evaluation ◽  
Physico Chemical ◽  
Biexponential Model

Nanocapsules are vesicular drug carriers constituted of an oil core, a polymeric wall, and surfactants. A general understanding about the influence of the polymeric wall of nanocapsules on the release profiles of drugs is not known. So, this work was devoted to characterize formulations prepared without polymer or containing it at different concentrations. The indomethacin ethyl ester was used as model and the strategy was based on its interfacial alkaline hydrolysis simulating a sink condition for the release. The antiedematogenic activity in rats for ester-loaded-nanocarriers was also evaluated. The nanocapsules (NC) and nanoemulsion (NE) presented particle sizes below 300 nm, polydispersity lower than 1.2 and pH around 5. SAXS analyses showed that the sorbitan monostearate is dissolved in the oil and the polymer presents regions of crystallinity independently on the PCL concentration. TEM analyses showed droplets (NE) and spherical particles (NC). The time for the total disappearance of the ester varied from 12 h to 24 h depending on the polymer concentration. The biexponential model showed that the indomethacin ester was essentially entrapped within the nanocarriers in an extension of 85 to 95%. The half-lives varied from 147 to 289 min for the sustained phases and from 3 to 6 min for the burst phases. The ester-loaded-NC showed significant antiedematogenic activity, while the ester-loaded-NE did not inhibit the carrageenin-induced paw edema. The nanocapsules promoted the absorption of the indomethacin ethyl ester and the presence of the polymer is important to achieve the pharmacological effect.

General summary

1969 ◽  
Vol 173 (1032) ◽  
pp. 443-445 ◽  
Keyword(s):  
Blood Clotting ◽  
Chemical Characterization ◽  
Clotting System ◽  
Red Cell Membrane ◽  
Isolation And Purification ◽  
Enzymatic Action ◽  
Physico Chemical ◽  
Made In

The blood clotting, fibrinolytic, renin–angiotensin, kinin forming systems, and complements are all systems of greater or lesser complexity. Complement with its nine components, with one of the components of human complement, C'1 consisting of three subcomponents, is the most complex; the intrinsic blood clotting mechanism running it a very close second, if not a dead heat. In addition, in each system it is necessary to consider varying number of inhibitors and activators. In no system have all the components of that system been fully characterized, although excellent progress on this score has been made in regard to the blood clotting system and complement. The need for the isolation, purification and thoroughgoing physico-chemical characterization of the components of the kinin forming system is pressing. For it is only by such characterization, and by reconstitution of the system with pure, individual components that the present hypothesis as to the sequence of reactions in this system will be supported or refuted, and the nature and significance of the reported heterogeneity of kallikrein (kininogenase) PF(dil) (PF), and kininogen, be understood. Similarly, it is only through isolation and purification that the nature of the plasminogen activators of plasma and of tissue will be ultimately clarified. Although such isolation and purification will not solve the questions of the in vivo nature and significance of these systems it will aid mightily in their solution. The enzymes in each of the five systems are broadly of the same kind, being mainly proteases, or peptidases. However, with complement recent evidence to be published shortly in the Journal of Immunology indicates, that in addition to the proteolytic activity of C'3, there is also an enzymatic action of either C'8 or C'9 on the phospholipids of the red cell membrane.

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