Enzyme-catalyzed hydrolysis of fibre sludge into reducing sugars

2013 ◽  
Vol 3 (4) ◽  
pp. 353-359
Author(s):  
Jana Holm ◽  
Ganna Zinchenko ◽  
Ulla Lassi ◽  
Audrey Hernoux-Villière ◽  
Johanna Kärkkäinen
Keyword(s):  
Reducing Sugars ◽  
Hydrolysis Of

Enhanced Hydrolysis of Cellulose to Reducing Sugars on Kaolinte Clay Activated by Mineral Acid

2021 ◽  
Author(s):  
Yuxiao Dong ◽  
Dongshen Tong ◽  
Laibin Ren ◽  
Xingtao Chen ◽  
Hao Zhang ◽  
...  
Keyword(s):  
Reducing Sugars ◽  
Mineral Acid ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Base-catalyzed Degradations of Carbohydrates. IX. β-Eliminations of 4-O-Substituted Hexopyranosiduronates

1975 ◽  
Vol 53 (14) ◽  
pp. 2182-2188 ◽  
Author(s):  
Gerald O. Aspinall ◽  
Thinnayam N. Krishnamurthy ◽  
Walter Mitura ◽  
Masuo Funabashi
Keyword(s):  
Acid Hydrolysis ◽  
Reducing Sugars ◽  
Model Compounds ◽  
Methyl Methyl ◽  
Hydrolysis Of

Two methylated disaccharides, methyl [methyl 4-O-(2,3,4,6-tetra-O-methyl-α-D-glucopyranosyl)-2,3-di-O-methyl-β-D-glucopyranosid]uronate (9) and methyl 6-O-(methyl 2,3,4-tri-O-methyl-α-D-galactopyranosyluronate)-2,3,4-tri-O-methyl-β-D-glucopyranoside (15) have been synthesized and used as model compounds for the study of the base-catalyzed β-elimination of 4-O-substituted hexopyranosiduronates without degradation of exposed reducing sugars and of the selective acid hydrolysis of hex-4-enopyranosiduronates.

Valorization of agro-industrial wastes to produce hydrolytic enzymes by fungal solid-state fermentation

2018 ◽  
Vol 37 (2) ◽  
pp. 149-156 ◽  
Author(s):  
C. Marzo ◽  
A.B. Díaz ◽  
I. Caro ◽  
A. Blandino
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Reducing Sugars ◽  
Raw Materials ◽  
Hydrolytic Enzymes ◽  
Industrial Wastes ◽  
Added Value ◽  
Orange Peels ◽  
Enzyme Cocktail ◽  
Hydrolysis Of

Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg-1 and 17,600 U·kg-1, respectively; while for ESBC the maximum values reached were 35,000 U·kg-1 and 28,000 U·kg-1, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydrolysis of ORA and ESBC than a commercial enzyme cocktail typically used for this purpose. In this case, maximum reducing sugars concentrations of 57 and 47 g·L-1 were measured after the enzymatic hydrolysis of ESBC and ORA, respectively.

scholarly journals Production of Sugar Feedstocks for Fermentation Processes from Selected Fast Growing Grasses

Energies ◽  
2019 ◽  
Vol 12 (16) ◽  
pp. 3129 ◽  
Author(s):  
Kamila Przybysz ◽  
Edyta Małachowska ◽  
Danuta Martyniak ◽  
Piotr Boruszewski ◽  
Halina Kalinowska ◽  
...  
Keyword(s):  
Reducing Sugars ◽  
Kraft Pulping ◽  
Cellulose Content ◽  
Potential Yield ◽  
Fermentation Processes ◽  
Fast Growing ◽  
Initial Substrate ◽  
Scientific Goal ◽  
Hydrolysis Of ◽  
Substrate Concentrations

This study showed that kraft cellulosic pulps from Miscanthus giganetus JM Greef and Deuter ex Hodk. and Renvoize, sweet sorghum and 5 other fast growing grasses may be easily enzymatically converted to glucose-rich sugar feedstocks. The scientific goal of the paper was to assess and compare the potential yield of hydrolysis and verify whether these grasses may be a source of sugars for fermentation processes. Kraft pulping was used as a pretreatment method and hydrolysis of the pulps was conducted using a commercial multienzyme preparation containing cellulases and xylanases at initial substrate concentrations of 0.476, 3.88 and 7.46% w/v, and 3 different enzyme loadings. Results showed that tall wheatgrass, striped tuber oat grass, tall fescue and smooth bromegrass may be efficiently converted to sugar feedstocks for biotechnology application, but that the simple reducing sugars yield is lower than for wood, due to lower cellulose content.

Production of Reducing Sugars from Hydrolysis of Napier Grass by Acid or Alkali

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Duangkanok Tanangteerapong ◽  
Thanawat Tunjaroensin ◽  
Parkpoom Trakun-ung ◽  
Khanita Kamwilaisak
Keyword(s):  
Reducing Sugars ◽  
Napier Grass ◽  
Hydrolysis Of

scholarly journals Heterotrophic and mixotrophic cultivation of Chlorella pyrenoidosa and the enzymatic hydrolysis of its biomass for the synthesis of third generation bioethanol

2014 ◽  
Author(s):  
Nisha Phour Dhull ◽  
Kshitiz Gupta ◽  
Sanjeev Kumar Soni
Keyword(s):  
Wheat Bran ◽  
Algal Biomass ◽  
Reducing Sugars ◽  
Carbon Sources ◽  
Chlorella Pyrenoidosa ◽  
Potential Candidate ◽  
Third Generation ◽  
Carbon Utilization ◽  
Enzyme Cocktail ◽  
Hydrolysis Of

The present study has been carried out with a view of evaluating a green alga Chlorella pyrenoidosa as a potential candidate for the production of reducing sugars using an enzyme cocktail of multiple carbohydrates produced on site for the fermentation into bioethanol. The ability of C. pyrenoidosa to grow similarly fast on different carbon sources and light has been studied in Fog’s medium in heterotrophic and mixotrophic cultures. The high cells densities of mixotrophic cultures demonstrated that the growth-stimulating effects of light and carbon utilization were better as compared to the effects of glucose in heterotrophic condition. Maximum biomass yield of 1.2 g/l was achieved with 1% Glucose and 0.2% KNO3 after 7 days of incubation at 28oC. The algal biomass was steam pretreated and hydrolyzed by a cocktail of multiple carbohydrases produced by solid state culture of a laboratory isolate belonging to Aspergillus sp. on wheat bran exhibiting the yields of 86, 35, 74, 1947, 61, 17000 and 1388 IU/g dry wheat bran for CMCase, FPase, β-glucosidase, xylanase, mannanase, α-amylase and glucoamylase respectively. The enzyme cocktail worked well in the hydrolysis of algal biomass at 50oC and produced total reducing sugars amounting to 429 mg/g of dried biomass revealing carbohydrate conversion efficiency of 96% after 48 h of hydrolysis. The released sugars may be fermented using suitable yeast strains for the production of third generation bioethanol.

scholarly journals Dilute acid hydrolysis of wastes of fruits from Amazon for ethanol production

2021 ◽  
Vol 8 (3) ◽  
pp. 221-234
Author(s):  
Flávia Fernandes ◽  
◽  
Amanda Farias ◽  
Livia Carneiro ◽  
Ralyvan Santos ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Reducing Sugars ◽  
Bactris Gasipaes ◽  
Euterpe Oleracea ◽  
Dilute Acid ◽  
Liquid Ratio ◽  
Dilute Acid Hydrolysis ◽  
Hydrolysis Time ◽  
Astrocaryum Aculeatum ◽  
Hydrolysis Of

<abstract> <p>This study carried out the screening of wastes from Amazon plants to produce hydrolysates with a high monosaccharides content for ethanol production. Initially, we hydrolyzed (diluted acid) Amazon wastes (peel from the fruit of <italic>Astrocaryum aculeatum</italic> Meyer, peel from the fruit of <italic>Bactris gasipaes</italic> Kunth, straw obtained from endocarp of the fruit of <italic>Euterpe oleracea</italic> Mart., peel from the fruit of <italic>Theobroma grandiflorum</italic> Schumann and peel from the root of <italic>Manihot esculenta</italic> Crant) to obtain hydrolysates with the high content of fermentable sugars. Then, we investigated by 2<sup>3</sup> factorial design the influence of the factors: a) hydrolysis time (min); b) H<sub>2</sub>SO<sub>4</sub>-to-waste ratio (g/g) and c) solid-to-liquid ratio (g/mL) in the variables reducing sugars and furans. The hydrolysis of the peel of the fruit of <italic>Bactris gasipaes</italic> resulted in the highest concentration of reducing sugars (23.7 g/L). After detoxification and concentration process, the <italic>Bactris gasipaes</italic> hydrolysate results in 96.7 g/L of reducing sugars largely fermentable (90%) by <italic>Saccharomyces cerevisiae</italic> PE-2. The experimental design demonstrated that the factors H<sub>2</sub>SO<sub>4</sub>-to-waste ratio (g/g) and solid-to-liquid ratio (g/mL) were the most significant affecting the final content of reducing sugars and furans in the hydrolysate of the peel of <italic>Bactris gasipaes</italic>. Hydrolysis time of 4.4 min, H<sub>2</sub>SO<sub>4</sub>-to-waste ratio of 0.63 g/g, and the solid-to-liquid ratio of 0.17 g/mL resulted in the concentration of reducing sugars of 49 g/L. This study shows the potential of peels from the fruit of <italic>Bactris gasipaes</italic> to produce ethanol.</p> </abstract>

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