Improved sugar recovery from enzymatic hydrolysis of Miscanthus sinensis by surfactant-mediated alkaline pretreatment

2021 ◽  
Author(s):  
Chun-Ming Xu ◽  
Yi-Fan Gao ◽  
Shan-Shan He ◽  
Kun Luo ◽  
Qiong Yan ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Alkaline Pretreatment ◽  
Miscanthus Sinensis ◽  
Sugar Recovery ◽  
Hydrolysis Of

scholarly journals A Two-Step Ferric Chloride and Dilute Alkaline Pretreatment for Enhancing Enzymatic Hydrolysis and Fermentable Sugar Recovery from Miscanthus sinensis

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1843
Author(s):  
Lingci Li ◽  
Peng Ye ◽  
Mengyu Chen ◽  
Shangyuan Tang ◽  
Ying Luo ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ferric Chloride ◽  
Morphological Changes ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Alkaline Pretreatment ◽  
Miscanthus Sinensis ◽  
Sugar Recovery ◽  
Step Process ◽  
Whole Process

A two-step process was proposed to enhance enzymatic hydrolysis of Miscanthus sinensis based on a comparative study of acid/alkaline pretreatments. Ferric chloride pretreatment (FP) effectively removed hemicellulose and recovered soluble sugars, but the enzymatic hydrolysis was not efficient. Dilute alkaline pretreatment (ALP) resulted in much better delignification and stronger morphological changes of the sample, making it more accessible to enzymes. While ALP obtained the highest sugar yield during enzymatic hydrolysis, the soluble sugar recovery from the pretreatment stage was still limited. Furthermore, a two-step ferric chloride and dilute alkaline pretreatment (F-ALP) has been successfully developed by effectively recovering soluble sugars in the first FP step and further removing lignin of the FP sample in the second ALP step to improve its enzymatic hydrolysis. As a result, the two-step process yielded the highest total sugar recovery (418.8 mg/g raw stalk) through the whole process.

Combination of hot compressed water treatment and wet disk milling for high sugar recovery yield in enzymatic hydrolysis of rice straw

2012 ◽  
Vol 104 ◽  
pp. 743-748 ◽  
Author(s):  
Akihiro Hideno ◽  
Hiroyuki Inoue ◽  
Takashi Yanagida ◽  
Kenichiro Tsukahara ◽  
Takashi Endo ◽  
...  
Keyword(s):  
Water Treatment ◽  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Sugar Recovery ◽  
Recovery Yield ◽  
High Sugar ◽  
Hot Compressed Water ◽  
Hydrolysis Of

Enhanced enzymatic hydrolysis of spruce by alkaline pretreatment at low temperature

2008 ◽  
Vol 99 (6) ◽  
pp. 1320-1328 ◽  
Author(s):  
Yulin Zhao ◽  
Ying Wang ◽  
J.Y. Zhu ◽  
Art Ragauskas ◽  
Yulin Deng
Keyword(s):  
Enzymatic Hydrolysis ◽  
Low Temperature ◽  
Alkaline Pretreatment ◽  
Hydrolysis Of

scholarly journals Effect of lignin-blocking agent on enzyme hydrolysis of acid pretreated hemp waste

RSC Advances ◽  
2021 ◽  
Vol 11 (36) ◽  
pp. 22025-22033
Author(s):  
Daehwan Kim ◽  
Chang Geun Yoo ◽  
Jurgen Schwarz ◽  
Sadanand Dhekney ◽  
Robert Kozak ◽  
...  
Keyword(s):  
Structural Change ◽  
Chemical Composition ◽  
Enzymatic Hydrolysis ◽  
Blocking Agent ◽  
Enzyme Hydrolysis ◽  
Sugar Recovery ◽  
Cellulose Conversion ◽  
Hydrolysis Of

Enzymatic hydrolysis of acid pretreated hemp wastes is evaluated for its chemical composition, structural change, and sugar recovery. Addition of BSA enhances the cellulose conversion by avoiding non-productive binding between enzymes and inhibitors.

scholarly journals Impact of Alkaline Pretreatment Condition on Enzymatic Hydrolysis of Sugarcane Bagasse and Pretreatment Cost

2021 ◽  
Author(s):  
Chaojun Wang ◽  
Wei Qi ◽  
Cuiyi Liang ◽  
Qiong Wang ◽  
Wen Wang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Linear Trend ◽  
Alkaline Pretreatment ◽  
Practical Application ◽  
Severity Factor ◽  
Pretreatment Condition ◽  
Maximum Value ◽  
Combined Severity Factor ◽  
Hydrolysis Of ◽  
Temperature Time

Abstract A combined severity factor (RCSF) which is usually used to evaluate the effectiveness of hydrothermal pretreatment at above 100 ºC had been developed to assess the influence of temperature, time and alkali loading on pretreatment and enzymatic hydrolysis of lignocellulose. It is not suitable for evaluating alkaline pretreatment effectiveness at lower than 100 ºC. According to the reported deducing process, this study modified the expression of RCSF = log[CnOH- x t x e(Tr-Tb)/14.75] as RCSF = log{COH- x t x e[-13700/(Tr+273)+36.2]} which is easier and more reasonable to assess the effectiveness of alkaline pretreatment. It showed that RCSF exhibited linear trend with lignin removal, and quadratic curve relation with enzymatic hydrolysis efficiency (EHE) at the same temperature. The EHE of alkali-treated SCB could attain the maximum value at lower RCSF, which indicated that it was not necessary to continuously enhance strength of alkaline pretreatment for improving EHE. Within a certain temperature range, the alkali loading was more important than temperature and time to influence pretreatment effectiveness and EHE. Furthermore, the contribution of temperature, time and alkali loading to pretreatment cost which was seldom concerned was investigated in this work. The alkali loading contributed more than 70% to the pretreatment cost. This study laid the foundation of further optimizing alkaline pretreatment to reduce cost for its practical application.

scholarly journals Process simulation for xylitol production from brewer’s spent grain in a Colombian biorefinery. Part 1: Xylose production from arabinoxilans extracted by the alkaline pretreatment of BSG

2019 ◽  
Vol 39 (1) ◽  
Author(s):  
Andrés Alfonso Gil Montenegro ◽  
Juan Sebastian Arocha Morales ◽  
Lilia Carolina Rojas Pérez ◽  
Paulo César Narváez Rincón
Keyword(s):  
Enzymatic Hydrolysis ◽  
Dry Weight ◽  
Alkaline Pretreatment ◽  
Raw Material ◽  
Brewing Industry ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
Two Stages ◽  
Xylose Production ◽  
Hydrolysis Of

This work presents the simulation in Aspen Plusr of a process to obtain arabinoxylans (AX) from Brewer’s Spent Grain (BSG), which is the major byproduct of the brewing industry. The process is divided into two stages: alkaline pretreatment and enzymatic hydrolysis. These stages cover the extraction of proteins and AX from BSG using an alkaline pretreatment and enzymatic hydrolysis of the AX separated from the liquid stream to obtain xylose, i.e. the substrate required for the fermentation to xylitol. Simulation results show that xylose obtained corresponds to 8,5% of the dry weight of the raw material, obtaining a yield of 58%. Several streams of byproducts were obtained, such as proteins, polypeptides, amino acids, phenolic compounds and lignocellulosic residues that can be valorized in other processes. Simulation was performed in the context of a biorefinery in Colombia.

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