BMP9 regulates cross-talk between breast cancer cells and bone marrow-derived mesenchymal stem cells

2014 ◽  
Vol 37 (5) ◽  
pp. 363-375 ◽  
Author(s):  
Shaoheng Wan ◽  
Yuehong Liu ◽  
Yaguang Weng ◽  
Wei Wang ◽  
Wei Ren ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cancer Cells ◽  
Cross Talk ◽  
Breast Cancer Cells

Exosomal MicroRNA-204 Derived from Bone Marrow Mesenchymal Stem Cells (BMSCs) Inhibits Cell Proliferation and Induces Apoptosis Through NF-κB Signaling Pathway in Breast Cancer

2022 ◽  
Vol 12 (2) ◽  
pp. 273-278
Author(s):  
Daqing Jiang ◽  
Xianxin Xie ◽  
Cong Wang ◽  
Weijie Li ◽  
Jianjun He
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Marrow Mesenchymal Stem Cells ◽  
Induced Apoptosis ◽  
Signaling Activation

Our study intends to assess the relationship between exosomes derived from bone marrow mesenchymal stem cells (BMSC-exo) and breast cancer. BMSC-exo were isolated and characterized by transmission electron microscopy. After transfection of BMSCs with miR-204 inhibitor, breast cancer cells were incubated with BMSC-exo followed by analysis of cell proliferation by CCK-8 assay, cell apoptosis by flow cytometry, and expression of apoptosis-related protein and NF-κB signaling by western blot. The co-culture of BMSC-exo with breast cancer cells enhanced miR-204 transcription, inhibited cell proliferation and induced apoptosis. Further, BMSC-exo accelerated apoptosis as demonstrated by the increased level of Bax and casepase-3 and decreased Bcl-2 expression, as well as reduced NF-κB signaling activity. But knockdown of miR-204 abolished the effect of BMSC-exo on apoptosis and proliferation with NF-κB signaling activation. In conclusion, miR-204 from BMSC-exo restrains growth of breast cancer cell and might be a novel target for treating breast cancer.

Mechanism of miR-506 Targeting Autophagy and Apoptosis-Associated Gene Beclin1 to Promote Bone Marrow Mesenchymal Stem Cells (BMSCs) Differentiation and Metastasis to Breast Cancer

2021 ◽  
Vol 11 (12) ◽  
pp. 2502-2506
Author(s):  
Qiumei Liu ◽  
Yanyan Wu ◽  
Jian Ye
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Luciferase Reporter ◽  
Differentiation Capacity ◽  
Marrow Mesenchymal Stem Cells ◽  
Metastasis To Breast

This study investigates miR-506 targeting the autophagy and apoptosis-related gene Beclin1 and analyzes the mechanism of its effect on bone marrow mesenchymal stem cells (BMSCs) differentiation and metastasis to breast cancer. Detection of miRNA-506 expression in BMSCs and breast cancer cells was done by Real-time PCR. A luciferase reporter system analyzed the targeting relationship between Beclin1 and miR-506. miR-NC group, BMSCs induction group, siRNA-NC group, and siRNA-Beclin1 group was set to measure Beclin1 expression, cell differentiation and migration by transwell assay, cell viability by MTT assay, proliferation by EdU staining and apoptosis and cycle by flow cell assay. miRNA-506 showed a high expression in breast cancer cells and low expression in BMSCs. miRNA-506 mimics significantly promote breast cancer cell proliferation which was inhibited by miRNA-506 inhibitors. The expression of Beclin1mRNA was significantly higher and miR-506 was lower in breast cancer cells. BMSCs induction significantly downregulated Beclin1 expression, increased miR-506 expression, and promoted cell invasive differentiation and metastatic capacity. In conclusion, elevated miR-506 expression was associated with decreased Beclin1 expression and increased metastatic differentiation capacity of breast cancer cells, which could effectively increase differentiation capacity and metastatic differentiation after induction by BMSCs.

scholarly journals The in vitro and in vivo effects of mesenchymal stem cells on breast cancer cells

2017 ◽  
Vol 28 ◽  
pp. i15
Author(s):  
T. Herheliuk ◽  
O. Perepelytsina ◽  
O. Yakymchuk ◽  
L. Ostapchenko ◽  
M. Sydorenko
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
In Vivo Effects

Abstract 2605: Exosomal transfer of microRNA and growth factors by human Mesenchymal Stem Cells support to breast cancer cells.

2013 ◽  
Author(s):  
Patrice Penfornis ◽  
Krishna C. Vallabhaneni ◽  
Francois Guillonneau ◽  
Griffin Orr ◽  
Santosh Dhule ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Human Mesenchymal Stem Cells

scholarly journals Perichondrium mesenchymal stem cells inhibit the growth of breast cancer cells via the DKK-1/Wnt/β-catenin signaling pathway

2016 ◽  
Vol 36 (2) ◽  
pp. 936-944 ◽  
Author(s):  
Min Li ◽  
Hui Cai ◽  
Ya Yang ◽  
Jia Zhang ◽  
Kai Sun ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cancer Cells ◽  
Signaling Pathway ◽  
Breast Cancer Cells
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