Insights into polyaromatic hydrocarbon biodegradation by Pseudomonas stutzeri CECT 930: operation at bioreactor scale and metabolic pathways

Microorganisms are tightly bounded to the animals on Earth. Bacteria, among other types of microbes, interact with their hosts in several ways regarding metabolic pathways, development, complex behavioral processes such as mate recognition, among others. The adult males of Leptonycteris yerbabuenae, a nectarivorous bat, develop an interscapular odoriferous patch during the mating season. Here we present a description of the microbiota associated to this sebaceous patch 11 adult males, and studied it in terms of their taxonomical information. The variability between samples was not relevant to this study, and the most abundant phyla were Firmicutes and Proteobacteria, with dominanting classes including Gammaproteobacteria, Clostridia and Bacilli. The two most abundant species were Aggregatibacter pneumotropica and Actinomyces europaeus and other Streptococcus minor, Pseudomonas stutzeri, P. viridiflava and Staphylococcus epidermis, which are relevant in both normal and wounded human skin. Furthermore, the species present in this mating organ are involved in metabolic pathways related to fatty acid transformation to volatile molecules, which could be playing a key role in mate recognition.

Download Full-text

Metal Toxicity Reduction in Naphthalene Biodegradation by Use of Metal-Chelating Adsorbents

Applied and Environmental Microbiology ◽

10.1128/aem.64.11.4610-4613.1998 ◽

1998 ◽

Vol 64 (11) ◽

pp. 4610-4613 ◽

Cited By ~ 35

Author(s):

Pomthong Malakul ◽

Keeran R. Srinivasan ◽

Henry Y. Wang

Keyword(s):

Metal Toxicity ◽

Chelating Resin ◽

Hydrocarbon Biodegradation ◽

Polyaromatic Hydrocarbon ◽

Metal Chelating ◽

Chelex 100 ◽

Toxicity Reduction ◽

Chelex Resin ◽

As Adsorption ◽

Naphthalene Biodegradation

ABSTRACT A model system comprising microbial degradation of naphthalene in the presence of cadmium has been developed to evaluate metal toxicity associated with polyaromatic hydrocarbon biodegradation and its reduction by the use of unmodified and surfactant-modified clays in comparison with a commercially available chelating resin (Chelex 100; Bio-Rad). The toxicity of cadmium associated with naphthalene biodegradation was shown to be reduced significantly by using the modified-clay complex and Chelex resin, while unmodified clay has no significant impact on this reduction. The degree of metal toxicity reduction can be quantitatively related to the metal adsorption characteristics of these adsorbents, such as adsorption capacity and selectivity.

Download Full-text

Oxidation of a Mixture of Polyaromatic Hydrocarbons by a Mixed Culture of Hydrocarbon-Oxidizing Microorganisms

Eurasian Chemico-Technological Journal ◽

10.18321/ectj1034 ◽

2021 ◽

Vol 23 (1) ◽

pp. 59

Author(s):

D. A. Filatov ◽

M. A. Kopytov ◽

V. S. Ovsyannikova ◽

E. A. Elchaninova

Keyword(s):

Liquid Medium ◽

Mixed Culture ◽

Polyaromatic Hydrocarbons ◽

Pseudomonas Stutzeri ◽

Polyaromatic Hydrocarbon ◽

Natural Conditions ◽

Biochemical Oxidation ◽

Oxygenase Activity ◽

Total Utilization ◽

Initial Contamination

The possibility of biochemical oxidation of polyaromatic hydrocarbon mixtures (PAHs) by the mixed culture of hydrocarbon-oxidizing microorganisms (HOM) in a liquid medium and soil was investigated. The mixed HOM culture was represented by Pseudomonas stutzeri, Pseudomonas putida, Bacillus cereus, and Arthrobacter globiformis genera. It was shown that during HOM cultivation of the microorganisms under study in the liquid medium their number increases from 0.25·104 to 11·108 CFU/ml, which is accompanied by an increase in their oxygenase activity. All PAHs identified were subjected to oxidation from 11.3 to 100%. The results of experiments on biodegradation of PAHs under natural conditions have shown that for 60 days the total utilization of oil products in soils was on the average 65% of the initial contamination. This suggests the prospects for the use of the mixed HOM culture under study for effective biodegradation of PAHs polluting soil and waste waters.

Download Full-text

Microbiome of the sexual scent organ of Leptonycteris yerbabuenae

10.7287/peerj.preprints.2627v1 ◽

2016 ◽

Author(s):

Osiris Gaona ◽

Daniel Cerqueda-García ◽

Daniel Martínez-Martínez ◽

Andrés Moya ◽

Luisa I. Falcón

Keyword(s):

Fatty Acid ◽

Human Skin ◽

Metabolic Pathways ◽

Pseudomonas Stutzeri ◽

Mate Recognition ◽

Abundant Species ◽

Adult Males ◽

Mating Season ◽

Staphylococcus Epidermis ◽

Leptonycteris Yerbabuenae

Microorganisms are tightly bounded to the animals on Earth. Bacteria, among other types of microbes, interact with their hosts in several ways regarding metabolic pathways, development, complex behavioral processes such as mate recognition, among others. The adult males of Leptonycteris yerbabuenae, a nectarivorous bat, develop an interscapular odoriferous patch during the mating season. Here we present a description of the microbiota associated to this sebaceous patch 11 adult males, and studied it in terms of their taxonomical information. The variability between samples was not relevant to this study, and the most abundant phyla were Firmicutes and Proteobacteria, with dominanting classes including Gammaproteobacteria, Clostridia and Bacilli. The two most abundant species were Aggregatibacter pneumotropica and Actinomyces europaeus and other Streptococcus minor, Pseudomonas stutzeri, P. viridiflava and Staphylococcus epidermis, which are relevant in both normal and wounded human skin. Furthermore, the species present in this mating organ are involved in metabolic pathways related to fatty acid transformation to volatile molecules, which could be playing a key role in mate recognition.

Download Full-text

The metabolic pathways and environmental controls of hydrocarbon biodegradation in marine ecosystems

10.3389/978-2-88919-346-2 ◽

2015 ◽

Keyword(s):

Metabolic Pathways ◽

Marine Ecosystems ◽

Hydrocarbon Biodegradation ◽

Environmental Controls

Download Full-text

Scientometric Analysis of Diesel Pollutions in Antarctic Territories: A Review of Causes and Potential Bioremediation Approaches

Sustainability ◽

10.3390/su13137064 ◽

2021 ◽

Vol 13 (13) ◽

pp. 7064

Author(s):

Ahmad Fareez Ahmad Roslee ◽

Siti Aqlima Ahmad ◽

Claudio Gomez-Fuentes ◽

Noor Azmi Shaharuddin ◽

Khalilah Abdul Khalil ◽

...

Keyword(s):

Metabolic Pathways ◽

Petroleum Hydrocarbons ◽

Anthropogenic Activities ◽

Hydrocarbon Biodegradation ◽

Scientometric Analysis ◽

Hydrocarbon Pollution ◽

The Past ◽

Antarctic Treaty ◽

Resources Exploitation ◽

The Antarctic

Despite the continuous enforcement of Antarctic Treaty System, ATS (1961), today Antarctica is constantly plagued by hydrocarbon pollution from both legacy and present-day wastes, especially near where anthropogenic activities are the most intense. The advances of science have led to multiple breakthroughs to bolster bioremediation techniques and revamp existing laws that prevent or limit the extent of hydrocarbon pollution in Antarctica. This review serves as the extension of collective efforts by the Antarctic communities through visual representation that summarizes decades of findings (circa 2000–2020) from various fields, pertinent to the application of microbe-mediated hydrocarbons remediation. A scientometric analysis was carried out based on indexed, scientific repositories (ScienceDirect and Scopus), encompassing various parameters, including but not limited to keywords co-occurrences, contributing countries, trends and current breakthroughs in polar researches. The emergence of keywords such as bioremediation, biosurfactants, petroleum hydrocarbons, biodiesel, metagenomics and Antarctic treaty policy portrays the dynamic shifts in Antarctic affairs during the last decades, which initially focused on exploration and resources exploitation before switching to scientific research and the more recent ecotourism. This review also presents the hydrocarbonoclastic microbes studied in the past, known and proposed metabolic pathways and genes related to hydrocarbon biodegradation as well as bacterial adaptations to low-temperature condition.

Download Full-text