scholarly journals Oxidation of a Mixture of Polyaromatic Hydrocarbons by a Mixed Culture of Hydrocarbon-Oxidizing Microorganisms

2021 ◽  
Vol 23 (1) ◽  
pp. 59
Author(s):  
D. A. Filatov ◽  
M. A. Kopytov ◽  
V. S. Ovsyannikova ◽  
E. A. Elchaninova
Keyword(s):  
Liquid Medium ◽  
Mixed Culture ◽  
Polyaromatic Hydrocarbons ◽  
Pseudomonas Stutzeri ◽  
Polyaromatic Hydrocarbon ◽  
Natural Conditions ◽  
Biochemical Oxidation ◽  
Oxygenase Activity ◽  
Total Utilization ◽  
Initial Contamination

The possibility of biochemical oxidation of polyaromatic hydrocarbon mixtures (PAHs) by the mixed culture of hydrocarbon-oxidizing microorganisms (HOM) in a liquid medium and soil was investigated. The mixed HOM culture was represented by Pseudomonas stutzeri, Pseudomonas putida, Bacillus cereus, and Arthrobacter globiformis genera. It was shown that during HOM cultivation of the microorganisms under study in the liquid medium their number increases from 0.25·104 to 11·108 CFU/ml, which is accompanied by an increase in their oxygenase activity. All PAHs identified were subjected to oxidation from 11.3 to 100%. The results of experiments on biodegradation of PAHs under natural conditions have shown that for 60 days the total utilization of oil products in soils was on the average 65% of the initial contamination. This suggests the prospects for the use of the mixed HOM culture under study for effective biodegradation of PAHs polluting soil and waste waters.

Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture

Chemosphere ◽  
2016 ◽  
Vol 156 ◽  
pp. 195-203 ◽  
Author(s):  
G. Briceño ◽  
H. Schalchli ◽  
O. Rubilar ◽  
G.R. Tortella ◽  
A. Mutis ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Mixed Culture

DIMERIZATION OF POLYAROMATIC HYDROCARBON MOLECULES WITH FORMATION OF E-BRIDGE BOND: A THEORETICAL STUDY

2020 ◽  
Author(s):  
A. S. Savchenkova ◽  
◽  
A. S. Semenikhin ◽  
I. V. Chechet ◽  
S. G. Matveev ◽  
...  
Keyword(s):  
Theoretical Study ◽  
Soot Formation ◽  
Polyaromatic Hydrocarbons ◽  
Combustion Engines ◽  
Polyaromatic Hydrocarbon ◽  
Subsequent Growth ◽  
Bridge Bond ◽  
Soot Particles ◽  
Fuel Burning ◽  
Hydrocarbon Molecules

In combustion engines and other fuel-burning devices, during the combustion of hydrocarbon fuels at a temperature of 1000-1400 K, soot is actively formed, deposited on the cold surfaces of the devices, which reduces their service life. At present, much attention is paid to the problems of controlling the amount and size of soot particles formed during combustion. However, the mechanism of soot formation has not yet been fully understood. It is assumed that under combustion conditions, young soot particles are formed by nucleation of aromatic and polyaromatic hydrocarbons (PAHs) with subsequent growth of particles due to the addition of new molecules.

Aflatoxin Production by Aspergillus parasiticus and Its Stability During the Manufacture of Korean Soy Paste (Doenjang) and Soy Sauce (Kanjang) by Traditional Method

1988 ◽  
Vol 51 (12) ◽  
pp. 938-944 ◽  
Author(s):  
KUN-YOUNG PARK ◽  
KYU-BOK LEE ◽  
LLOYD B. BULLERMAN
Keyword(s):  
Bacillus Subtilis ◽  
Mixed Culture ◽  
Traditional Method ◽  
Degradation Rate ◽  
Aspergillus Parasiticus ◽  
Aflatoxin Production ◽  
Toxin Production ◽  
Soy Sauce ◽  
Natural Conditions ◽  
Soybean Cake

Aflatoxin (AF) production and its stability on meju (crushed Korean soybean cake) by a pure culture of Aspergillus parasiticus and a mixed culture of A. parasiticus. A. oryzae and Bacillus subtilis (fermentation 1) during the manufacture by traditional methods of Korean soy paste (doenjang) and soy sauce (kanjang) were studied. There was a difference in the amount of aflatoxin production on different varieties of soybeans, but the pattern of toxin production was similar. During fermentation, more total aflatoxins were produced under the mixed culture condition. Aflatoxin G1 (AFG1) production was highly stimulated though it degraded quickly, whereas aflatoxin B1 (AFB1) synthesis was low. The exposure of the meju to sunlight during fermentation had no effect in reducing aflatoxin synthesis. When the meju fermented under natural conditions (fermentation 2) with a contamination by A. parasiticus, high levels of aflatoxins were still produced. After a month of ripening of the fermented meju with charcoal in brine, more AFG1 than AFB1 was degraded in both the pure and mixed culture samples (degradation %; B1:2–69%, G1:31–84%). When the meju was ripened in water, most of the aflatoxins were degraded (B1:95–99%, G1:100%) in a month, accompanied by a significant increase in pH (p<0.05). A greater amount of aflatoxins (96–100%) was detected in the meju when it was ripened in brine, however, lower amounts (25–85%) of aflatoxins remained in meju ripened in water. During three months of ripening in brine, 83–98% of AFB1 and 98–100% of AFG1 were degraded in fermentation 1, but the degradation rate was slower (B1 :63%, G1:98%) following fermentation 2. The total levels of aflatoxins remaining were significantly (p<0.05) reduced when charcoal was added to the mixture.

Biochemical oxidation of polyaromatic compounds by hydrocarbon-oxidizing bacteria in a liquid medium

2016 ◽  
Vol 56 (7) ◽  
pp. 657-662 ◽  
Author(s):  
D. A. Filatov ◽  
E. A. El’chaninova ◽  
V. S. Ovsyannikova ◽  
L. K. Altunina
Keyword(s):  
Liquid Medium ◽  
Biochemical Oxidation

scholarly journals Chlorpyrifos removal by Thiobacillus sp. and Clostridium sp. in liquid medium

2018 ◽  
Vol 197 ◽  
pp. 13012
Author(s):  
Thalia Sunaryo ◽  
Hilarion Widyatmoko ◽  
Astri Rinanti
Keyword(s):  
Gas Chromatography ◽  
Liquid Medium ◽  
Temperature Variation ◽  
Mixed Culture ◽  
Removal Efficiency ◽  
Salt Solution ◽  
Contact Time ◽  
Mineral Salt ◽  
Ms Analysis

The bioremediation research of chlorpyrifos has been done to remove the concentration of chlorphyrifos using the natural bacterial Thiobacillus sp. and Clostridium sp.. The efficiency of chlorpyrifos removal was determined by the temperature and contact time. The research was done by adding 100 ppm of chlorpyrifos into the Stone Mineral Salt solution (SMSs) under controlled condition, then each bacterial are added as much as 10% (v/v) with pH 7. To obtain optimum efficiency, this study was conducted with temperature variation (°C) 25, 30, 35, 40 and contact time (hours) 12, 24, 36, 48. Based on Gas Chromatography Mass Spektrofotometer (GC-MS) analysis, the efficiency of removal at temperature (°C) 25, 30, 35, 40 are 43%, 68%, 71% and 52% respectively, while the removal efficiency at contact time (hours) 12, 24, 36, 48 are 43%, 49%, 74%, and 36%. The result showed that 74% removal efficiency by Thiobacillus sp. and Clostridium sp. obtained at 35°C in 36 hours of contact time. Thus, the mixed culture of Thiobacillus sp. and Clostridium sp. are able to synergize for removing the chlorpyrifos at 100 ppm.

scholarly journals Isolation, Selection and Identification of Polyaromatic Hydrocarbons (PAHs) Degrading Bacteria from Heavy Oil Waste (HOW)-Contaminated Soil

2020 ◽  
Vol 27 (2) ◽  
pp. 142
Author(s):  
Mohamad Yani ◽  
Charlena Charlena ◽  
Zainal Alim Mas’ud ◽  
Iswandi Anas ◽  
Yadi Setiadi ◽  
...  
Keyword(s):  
Heavy Oil ◽  
Contaminated Soil ◽  
Polyaromatic Hydrocarbons ◽  
Polyaromatic Hydrocarbon ◽  
Ochrobactrum Anthropi ◽  
Marine Agar ◽  
Emulsification Activity ◽  
Degrading Bacteria ◽  
Agar Media ◽  
Ph Range

The heavy oil waste (HOW) containing polyaromatic hydrocarbon (PAHs) is a persistent organic pollutants (POPs) that difficult to degrade. The new PAH degrading consortium was investigated from HOW contaminated soil in North Sumatera of  Indonesia. The isolation, selection and identification of polyaromatic hydrocarbon degrading bacteria from soil contaminated by HOW was conducted to solve a bioremediation process. The isolation microbes from soil contaminated by HOW was performed using a minimum ONR7a media and followed on marine agar media for purification purposes. From the performed isolation results, 11 isolates were able to degrade PAHs compounds, such as phenanthrene, dibenzothiophene, or fluorene compounds. They grew at pH range of 4.8-8.2 and performed on emulsification activity in paraffin from 0.150-0.662. Three of them showed the best performance on HOW biodegradation capability and then successfully selected and identified as Salipiger sp., Bacillus altitudinis, and Ochrobactrum anthropi. using 16S rDNA. The HOW biodegradation as TPH-degradation were 38.66%, 59.60%, and 47.16%, respectively. Those isolated bacteria could potentially be as bioremediation agents to develop on bioremediation process for soils contaminated by HOW.

MICROBIOLOGICAL FORMATION OF BASIC FERRIC SULFATES

1973 ◽  
Vol 53 (3) ◽  
pp. 315-323 ◽  
Author(s):  
K. C. IVARSON
Keyword(s):  
Liquid Medium ◽  
Ferrous Sulfate ◽  
Metamorphic Rock ◽  
Solid Medium ◽  
Thiobacillus Ferrooxidans ◽  
Ferric Sulfate ◽  
Uranium Mine ◽  
Natural Conditions

The iron-oxidizing bacterium Thiobacillus ferrooxidans was isolated from yellow-brown deposits found in soil, shale, metamorphic rock, and a uranium mine. In addition to the bacterium and pyrite, jarosite (K Fe3(SO4)2(OH)6), a basic ferric sulfate, was found to be present in the deposits. Oxidation of ferrous sulfate by the organism in liquid medium resulted in the formation of jarosite. On a solid medium of agar containing ferrous sulfate, ammoniojarosite (NH4Fe3(SO4)2(OH)6) was formed. Because jarosite can be synthesized at 25 C and 1 atm, it" is suggested that under natural conditions T. ferrooxidans plays a role in the formation of basic ferric sulfates.

INTERACTION OF THE DIOXYGEN MOLECULE WITH THE C96H24 POLYAROMATIC HYDROCARBON CLUSTER: A QUANTUM CHEMICAL INSIGHT

2008 ◽  
Vol 22 (13) ◽  
pp. 2115-2127
Author(s):  
VOLODYMYR D. KHAVRYUCHENKO ◽  
YURIJ A. TARASENKO ◽  
VOLODYMYR V. STRELKO ◽  
OLEKSIY V. KHAVRYUCHENKO ◽  
VLADYSLAV V. LISNYAK
Keyword(s):  
Quantum Chemical ◽  
Polyaromatic Hydrocarbons ◽  
Quantum Chemical Study ◽  
Chemical Study ◽  
High Multiplicity ◽  
Polyaromatic Hydrocarbon ◽  
Superoxide Ion ◽  
Cluster A ◽  
Modern Phys

Interaction of the previously described [V. D. Khavryuchenko, Y. A. Tarasenko, V. V. Strelko, O. V. Khavryuchenko and V. V. Lisnyak, Quantum chemical study of polyaromatic hydrocarbons in high multiplicity states, Int. J. Modern. Phys. B21, 4507 (2007), in press] polyaromatic hydrocarbon (PAH) C 96 H 24 with dioxygen molecule and KO2 have been quantum chemically examined. The probability of existence of the oxygen superoxide ion-radical O 2 adsorbed on the surface of the PAH is critically discussed.

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