Angiogenic potential of extracellular matrix of human amniotic membrane

Periodontitis is a prevalent disease characterized by the loss of periodontal supporting tissues, bone, periodontal ligament, and cementum. The application of a bone tissue engineering strategy with Decellularized Human Amniotic Membrane (DAM) with adipose-derived stromal cells (ASCs) has shown to be convenient and valuable. This study aims to investigate the treatments of a rat periodontal furcation defect model with DAM, ASCs, and a mineralized extracellular matrix (ECM). Rat ASCs were expanded, cultivated on DAM, and with a bone differentiation medium for four weeks, deposited ECM on DAM. Periodontal healing for four weeks was evaluated by micro-computed tomography and histological analysis after treatments with DAM, ASCs, and ECM and compared to untreated defects on five consecutive horizontal levels, from gingival to apical. The results demonstrate that DAM preserves its structure during cultivation and healing periods, supporting cell attachment, permeation, bone deposition on DAM, and periodontal regeneration. DAM and DAM+ASCs enhance bone healing compared to the control on the gingival level. In conclusion, DAM with ASC or without cells and the ECM ensures bone tissue healing. The membrane supported neovascularization and promoted osteoconduction.

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Human amniotic membrane extracellular matrix scaffold for dental pulp regeneration in vitro and in vivo

International Endodontic Journal ◽

10.1111/iej.13675 ◽

2021 ◽

Author(s):

Hengameh Bakhtiar ◽

Azin Ashoori ◽

Sarah Rajabi ◽

Mohamad Pezeshki‐Modaress ◽

Alireza Ayati ◽

...

Keyword(s):

Extracellular Matrix ◽

Amniotic Membrane ◽

Dental Pulp ◽

Human Amniotic Membrane ◽

Pulp Regeneration ◽

Regeneration In Vitro ◽

Extracellular Matrix Scaffold

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Human Amniotic Membrane Enriched with Urinary Bladder Fibroblasts Promote the Re-Epithelization of Urothelial Injury

Cell Transplantation ◽

10.1177/0963689720946668 ◽

2020 ◽

Vol 29 ◽

pp. 096368972094666

Author(s):

Urška Dragin Jerman ◽

Peter Veranič ◽

Tina Cirman ◽

Mateja Erdani Kreft

Keyword(s):

Extracellular Matrix ◽

Amniotic Membrane ◽

Wound Dressing ◽

Ex Vivo ◽

Cell Junctions ◽

Human Amniotic Membrane ◽

Urothelial Cells ◽

Ex Vivo Model ◽

Bladder Tissue ◽

Extracellular Matrix Components

Culturing cells in three-dimensional systems that include extracellular matrix components and different cell types mimic the native tissue and as such provide much more representative results than conventional two-dimensional cell cultures. In order to develop biomimetic bladder tissue in vitro, we used human amniotic membrane (AM) extracellular matrix as a scaffold for bladder fibroblasts (BFs) and urothelial cells. Our aims were to evaluate the integration of BFs into the AM stroma, to assess the differentiation of the urothelium on BFs-enriched AM scaffolds, and to evaluate the AM as a urothelial wound dressing. First, to achieve the optimal integration of BFs into AM stroma, different intact and de- epithelialized AM (dAM) scaffolds were tested. BFs secreted matrix metalloproteinase (MMP)-1 and MMP-2 and integrated into the stroma of all types of AM scaffolds. Second, to establish urothelial tissue equivalent, urothelial cells were seeded on dAM scaffolds enriched with BFs. The BFs in the stroma of the AM scaffolds promoted (1) the proliferation of urothelial cells, (2) the attachment of urothelial cells on AM basal lamina with hemidesmosomes, and (3) development of multilayered urothelium with expressed uroplakins and well-developed cell junctions. Third, we established an ex vivo model of the injured bladder to evaluate the dAM as a wound dressing for urothelial full-thickness injury. dAM acted as a promising wound dressing since it enabled rapid re-epithelization of urothelial injury and integrated into the bladder tissue. Herein, the developed urothelial tissue equivalents enable further mechanistic studies of bladder epithelial–mesenchymal interactions, and they could be applied as biomimetic models for preclinical testing of newly developed drugs. Moreover, we could hypothesize that AM may be suitable as a dressing of the wound that occurs during transurethral resection of bladder tumor, since it could diminish the possibility of tumor recurrence, by promoting the rapid re-epithelization of the urothelium.

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Engraftment of bioengineered three-dimensional scaffold from human amniotic membrane-derived extracellular matrix accelerates ischemic diabetic wound healing

Archives of Dermatological Research ◽

10.1007/s00403-020-02137-3 ◽

2020 ◽

Author(s):

Davood Nasiry ◽

Ali Reza Khalatbary ◽

Mohammad-Amin Abdollahifar ◽

Abdollah Amini ◽

Mohammad Bayat ◽

...

Keyword(s):

Extracellular Matrix ◽

Wound Healing ◽

Amniotic Membrane ◽

Three Dimensional ◽

Human Amniotic Membrane ◽

Diabetic Wound ◽

Diabetic Wound Healing

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Could human amniotic membrane be a source for acupoint thread embedding therapy?

The Moldovan Medical Journal ◽

10.52418/moldovan-med-j.64-6.21.08 ◽

2021 ◽

Vol 64 (6) ◽

pp. 41-48

Author(s):

Olga Ignatov ◽

◽

Adrian Melnic ◽

Vitalie Procopciuc ◽

Viorica Mihaluta ◽

...

Keyword(s):

Extracellular Matrix ◽

Amniotic Membrane ◽

Subcutaneous Tissue ◽

Biomechanical Properties ◽

Functional Restoration ◽

Matrix Proteins ◽

Human Amniotic Membrane ◽

Ethical Problems ◽

Motor Disability ◽

Embedding Therapy

Background: Peripheral neuropathy usually leads to a major cause of motor disability, but the functional restoration after treatment continues to show modest results. Acupoint thread-embedding therapy is a subtype of acupuncture treatment in which biodegradable threads are inserted into skin, subcutaneous tissue or muscles at specific points for long stimulation. Different biodegradable materials have been developed and widely used. Human amniotic membrane is rich in collagen, extracellular matrix proteins and growth factors. The avascular, low immunogenic, anti-inflammatory, antibacterial, anti-fibrotic and non-tumorigenic properties of amniotic membrane make it valuable in medical applications and its use has no ethical problems. Elasticity, stiffness and other biomechanical properties also make it possible to use the amniotic membrane for various medical purposes. AM is almost always considered as discarded substance, it satisfies most of the criteria of an ideal biological tissue and shows almost zero rejection phenomenon. Conclusions: The human amniotic membrane, the cellular compounds and extracellular matrix have a lot of benefic proprieties that are or could be used in treatment of many human diseases. Its biological and biomechanical properties are promising in the manufacture and use of filaments in acupoint thread embedding therapy.

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