Evaluation of a point-of-care molecular detection device for Leishmania spp. and intercurrent fungal and mycobacterial organisms in Peruvian patients with cutaneous ulcers

Infection ◽  
2021 ◽  
Author(s):  
Ruwandi Kariyawasam ◽  
Braulio M. Valencia ◽  
Rachel Lau ◽  
Eric Shao ◽  
Courtney A. Thompson ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Point Of Care ◽  
Leishmania Spp

scholarly journals Aptamer Based Point of Care Diagnostic for the Detection of Food Allergens

2021 ◽  
Author(s):  
Sarah Stidham ◽  
Valerie Villareal ◽  
Vasant Chellappa ◽  
Lucas Yoder ◽  
Olivia Alley ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Detection Method ◽  
Point Of Care ◽  
Protein Detection ◽  
Peanut Protein ◽  
The Novel ◽  
Children And Families ◽  
Peanut Allergens ◽  
Consumer Device ◽  
Detection Technologies

Abstract Aptamers, due to their small size, strong target affinity, and ease of chemical modification, are ideally suited for molecular detection technologies. Here, we describe successful use of aptamer technology in a consumer device for the detection of peanut antigen in food. The novel aptamer-based protein detection method is robust across a wide variety of food matrices and sensitive to peanut protein at concentrations as low as 12.5 ppm (37.5 µg peanut protein in the sample). Integration of the assay into a sensitive, stable, and consumer friendly portable device will empower users to easily and quickly assess the presence of peanut allergens in foods before eating. With most food reactions occurring outside the home, the type of technology described here has significant potential to improve lives for children and families.

scholarly journals Molecular and parasitological detection ofLeishmania spp. in a dipteran of the speciesTabanus importunus

2013 ◽  
Vol 22 (4) ◽  
pp. 605-607 ◽  
Author(s):  
Willian Marinho Dourado Coelho ◽  
Katia Denise Saraiva Bresciani
Keyword(s):  
Visceral Leishmaniasis ◽  
Deoxyribonucleic Acid ◽  
Molecular Detection ◽  
Lutzomyia Longipalpis ◽  
Sand Flies ◽  
Thoracic Region ◽  
Main Species ◽  
Leishmania Spp ◽  
Genus Leishmania ◽  
Horse Fly

Leishmaniasis is an important chronic zoonosis caused by protozoa of the genus Leishmania spp. The major vectors of this protozoosis are sand flies, and Lutzomyia longipalpis is considered the main species implicated in the transmission of American Visceral Leishmaniasis in Brazil. The presence of the parasite's deoxyribonucleic acid (DNA) in ectoparasites such as ticks and fleas has prompted speculations about the existence of new vectors in the cycle of leishmaniasis. The aim of this paper is to report the molecular detection of Leishmania spp. in a horse fly of the species Tabanus importunus which parasitized an oligosymptomatic dog infected with Leishmania spp. Molecular amplification of the protozoan's DNA in the head, thoracic region and abdomen of the tabanid tested positive for Leishmania complex. This is the first report of the presence of DNA from Leishmaniaspp. in dipterous insects of the species T. importunus.

scholarly journals Molecular detection of Leishmania spp. in dogs and a cat from Doha, Qatar

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Clara Lima ◽  
Vito Colella ◽  
Maria Stefania Latrofa ◽  
Luís Cardoso ◽  
Domenico Otranto ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Leishmania Spp

scholarly journals Molecular detection of Leishmania spp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil

2014 ◽  
Vol 20 (1) ◽  
pp. 27 ◽  
Author(s):  
Virginia Richini-Pereira ◽  
Pamela Marson ◽  
Enio Hayasaka ◽  
Cassiano Victoria ◽  
Rodrigo da Silva ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Sao Paulo ◽  
The State ◽  
São Paulo ◽  
Wild Mammals ◽  
Western Area ◽  
Leishmania Spp

scholarly journals Instrument-Free Point-of-Care Molecular Detection of Zika Virus

2016 ◽  
Vol 88 (14) ◽  
pp. 7289-7294 ◽  
Author(s):  
Jinzhao Song ◽  
Michael G. Mauk ◽  
Brent A. Hackett ◽  
Sara Cherry ◽  
Haim H. Bau ◽  
...  
Keyword(s):  
Zika Virus ◽  
Molecular Detection ◽  
Point Of Care

Seroprevalence and molecular detection of Leishmania spp. in cats of West Aegean Region, Turkey

2021 ◽  
pp. 100573
Author(s):  
Ayça Aksulu ◽  
Hüseyin Bilgin Bilgiç ◽  
Tülin Karagenç ◽  
Serkan Bakırcı
Keyword(s):  
Molecular Detection ◽  
Aegean Region ◽  
Leishmania Spp

Installing CRISPR–Cas12a sensors in a portable glucose meter for point-of-care detection of analytes

The Analyst ◽  
2021 ◽  
Author(s):  
Ran Liu ◽  
Ying He ◽  
Tian Lan ◽  
Jingjing Zhang
Keyword(s):  
Molecular Detection ◽  
Point Of Care ◽  
Glucose Test ◽  
Glucose Meter ◽  
Point Of Care Detection

A simple target-responsive CRISPR/Cas12a-based strategy for translating molecular detection into a glucose test.

scholarly journals An Integrated Self-Powered 3D printed Sample Concentrator for Highly Sensitive Molecular Detection of HIV in Whole Blood at the Point of Care

The Analyst ◽  
2021 ◽  
Author(s):  
Karteek Kadimisetty ◽  
Kun Yin ◽  
Aoife M. Roche ◽  
Yanjie Yi ◽  
Frederic D. Bushman ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Infectious Diseases ◽  
Whole Blood ◽  
Biological Sample ◽  
Molecular Detection ◽  
Point Of Care ◽  
Highly Sensitive ◽  
3D Printed ◽  
Self Powered

Rapid and efficient biological sample preparation and pretreatment are crucial for highly sensitive, reliable and reproducible molecular detection of infectious diseases. Herein, we report a self-powered, integrated sample concentrator (SPISC)...

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