Experimental Studies on the Effect of Chlorpyriphos and Lead Acetate on Biochemical Parameters in Wistar Rats with Special Reference to Ameliorative Effect of Vitamin C

2013 ◽  
Vol 84 (4) ◽  
pp. 961-968
Author(s):  
Nisar Ahmad Nisar ◽  
Mudasir Sultana ◽  
Rajinder Raina ◽  
Hina Ashraf Waiz ◽  
Naseer Ahmad Baba ◽  
...  
Keyword(s):  
Vitamin C ◽  
Special Reference ◽  
Lead Acetate ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Experimental Studies ◽  
Ameliorative Effect

Ameliorative effect of vitamin C on the haematological changes induced by exposure of chlorpyriphos and lead acetate in Wistar rats

2013 ◽  
Vol 23 (4) ◽  
pp. 829-834
Author(s):  
Nisar Ahmad Nisar ◽  
Mudasir Sultana ◽  
Naseer Ahmad Baba ◽  
Parveez Ahmad Para ◽  
Hina Ashraf Waiz ◽  
...  
Keyword(s):  
Vitamin C ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Ameliorative Effect ◽  
Haematological Changes

scholarly journals Effects of Aqueous Extract of Ocimum gratissimum Leaves and Vitamin C on Lead Acetate-induced Changes in the Thymus of Adult Wistar Rats

2019 ◽  
pp. 1-9
Author(s):  
G. N. Okechukwu ◽  
E. Ezor ◽  
E. Finbarrs-Bello ◽  
L. N. Ebube ◽  
G. C. Uzomba ◽  
...  
Keyword(s):  
Vitamin C ◽  
Aqueous Extract ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Lead Toxicity ◽  
Wistar Rat ◽  
Ocimum Gratissimum ◽  
Ameliorative Effect ◽  
Male Wistar Rats ◽  
Induced Changes

Lead is a heavy metal found in earth’s crust. It is a widespread and insidious environmental toxin known as a severe and aggressive contaminant to human and animal organisms’ health status. This work is aimed at evaluating the effect of aqueous extract of Ocimum gratissimum (OG) on lead induced changes in the thymus of adult albino wistar rats. Thirty five male Wistar rats were used in the study and were randomly divided into seven groups with five rats in each group. The rats in Group one (G1) served as the Control and received distilled water. Group 2 received 120 mg/kg body weight (bwt) of lead acetate, G3 received 375 mg/kg bwt of OG only. G4 received 120 mg/kg bwt of lead acetate and OG extract at 375 mg/kg bwt., G5 received 120 mg/kg bwt of lead and OG at 750 mg/kg bwt. G6 received 375 mg/kg bwt of OG in two weeks followed by 120 mg/kg bwt of lead acetate for one week, while G7 received 120 mg/kg bwt of lead acetate and Vitamin C at 11900 mg/kg. All the administrations were carried out orally for twenty one (21) days. At the end of the administration, the rats were fasted for 24 hours. They weighted and humanely sacrificed via cervical dislocation. The thymus were harvested in all the groups and prepared for histological studies using routine haematoxylin and eosin (H&E) staining techniques. The result of the present study shows that lead possessed a distortive effect in the histoarchitecture of thymus of the wistar rat in G2 when compared with G1. Treatment of these experimental animals with vitamin C prove to posses more ameliorative effect in restoring the histoarchitecture on lead toxicity in the thymus closed to normal than OG. Therefore, it is suggested that aqueous extract OG can act via the same pathway as vitamin C, in maintaining the normal histological structures of the thymus of adult albino wistar rat exposed to lead toxicity.

scholarly journals Ameliorative Effect of Vitamin C on Alterations in Thyroid Hormones Concentrations Induced by Subchronic Coadministration of Chlorpyrifos and Lead in Wistar Rats

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Suleiman F. Ambali ◽  
Chinedu Orieji ◽  
Woziri O. Abubakar ◽  
Muftau Shittu ◽  
Mohammed U. Kawu
Keyword(s):  
Vitamin C ◽  
Thyroid Hormones ◽  
Wistar Rats ◽  
Low Dose ◽  
Antioxidant Properties ◽  
Thyroid Stimulating Hormone ◽  
Group Iv ◽  
Group Iii ◽  
Ameliorative Effect ◽  
Soya Oil

The present study evaluated the ameliorative effect of vitamin C on alteration in thyroid hormones induced by low-dose subchronic coadministration of chlorpyrifos (CPF) and lead (Pb). Forty Wistar rats were divided into 4 groups of 10 animals each. Groups I and II were administered soya oil (2 mL/kg) and vitamin C (100 mg/kg), respectively. Group III was coadministered CPF (4.25 mg/kg ~1/20th LD50) and Pb (250 mg/kg ~1/20th LD50), respectively. Group IV was pretreated with vitamin C (100 mg/kg) and then coadministered with CPF (4.25 mg/kg) and Pb (250 mg/kg), 30 min later. The regimens were administered by gavage for a period of 9 weeks. The marginal decrease in serum triiodothyronine and thyroxine and the significant increase in the concentrations of thyroid stimulating hormone and malonaldehyde in the group coadministered with CPF and Pb were ameliorated by vitamin C partly due to its antioxidant properties.

scholarly journals Beneficial Effect Administration of Vitamin C in Amelioration of Lead Hepatotoxicity

2012 ◽  
Vol 4 (3) ◽  
pp. 07-13 ◽  
Author(s):  
Nadia AIT HAMADOUCHE ◽  
Miloud SLIMANI ◽  
Abdelkader AOUES
Keyword(s):  
Lipid Peroxidation ◽  
Drinking Water ◽  
Vitamin C ◽  
Lead Acetate ◽  
Metal Content ◽  
Protective Action ◽  
Venous Blood ◽  
Lipid Hydroperoxide ◽  
Experimental Studies ◽  
Male Rats

Previous human and experimental studies have demonstrated that lead exposure may modify the metabolism of lipid. Oxidative stress with subsequent lipid peroxidation has been postulated as one mechanism for lead toxicity. The protective action of vitamins C against lead affects lipid hydroperoxide level and liver functions in male rats has been studied. Experiments were performed on male waster rats with body weights of 120-160 g. Male wistar rats were exposed to 3 g/l lead acetate in drinking water for 5 weeks and treated thereafter with vitamin C (500 mg/kg, orally) for 28 days. One day after the feeding was over, venous blood samples, under chloroform anesthesia, were collected. The animals were killed by exsanguinations and the liver was excise for determination the metal content and histopathological changes. Similarly, the tissue lipid (lipid peroxidation) and the enzyme fraction (superoxide dismutase (SOD), catalase (CAT), alkaline phosphatase (ALP), acid phosphatase (ACP) and glutathione (GSH) were also measured in the liver. Metal content in blood and liver was determined by means of atomic absorption spectrophotometry. Administration of lead acetate (3 g/l) in drinking water for 5 weeks induced a significant increase in the levels of hepatic ALP, ACP and lipid peroxidation. Lead acetate exposure also produced detrimental effects on the redox status of the liver indicated by a significant decline in the levels of liver antioxidants such SOD, CAT and GSH. Further, there was a significant increase in the levels of lead in blood and liver of animals exposed to lead. However, oral administration of vitamin C at dose level of 500 mg/kg body weight reduced the alterations in the previous parameters. Histological examination of the liver also revealed pathophysiological changes in lead acetate-exposed group and treatment with vitamin C improved liver histology. The result of this study strongly indicate that vitamin C has got a potent antioxidant action against lead acetate induced hepatic damage in rats.

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