scholarly journals Effects of Aqueous Extract of Ocimum gratissimum Leaves and Vitamin C on Lead Acetate-induced Changes in the Thymus of Adult Wistar Rats

2019 ◽  
pp. 1-9
Author(s):  
G. N. Okechukwu ◽  
E. Ezor ◽  
E. Finbarrs-Bello ◽  
L. N. Ebube ◽  
G. C. Uzomba ◽  
...  
Keyword(s):  
Vitamin C ◽  
Aqueous Extract ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Lead Toxicity ◽  
Wistar Rat ◽  
Ocimum Gratissimum ◽  
Ameliorative Effect ◽  
Male Wistar Rats ◽  
Induced Changes

Lead is a heavy metal found in earth’s crust. It is a widespread and insidious environmental toxin known as a severe and aggressive contaminant to human and animal organisms’ health status. This work is aimed at evaluating the effect of aqueous extract of Ocimum gratissimum (OG) on lead induced changes in the thymus of adult albino wistar rats. Thirty five male Wistar rats were used in the study and were randomly divided into seven groups with five rats in each group. The rats in Group one (G1) served as the Control and received distilled water. Group 2 received 120 mg/kg body weight (bwt) of lead acetate, G3 received 375 mg/kg bwt of OG only. G4 received 120 mg/kg bwt of lead acetate and OG extract at 375 mg/kg bwt., G5 received 120 mg/kg bwt of lead and OG at 750 mg/kg bwt. G6 received 375 mg/kg bwt of OG in two weeks followed by 120 mg/kg bwt of lead acetate for one week, while G7 received 120 mg/kg bwt of lead acetate and Vitamin C at 11900 mg/kg. All the administrations were carried out orally for twenty one (21) days. At the end of the administration, the rats were fasted for 24 hours. They weighted and humanely sacrificed via cervical dislocation. The thymus were harvested in all the groups and prepared for histological studies using routine haematoxylin and eosin (H&E) staining techniques. The result of the present study shows that lead possessed a distortive effect in the histoarchitecture of thymus of the wistar rat in G2 when compared with G1. Treatment of these experimental animals with vitamin C prove to posses more ameliorative effect in restoring the histoarchitecture on lead toxicity in the thymus closed to normal than OG. Therefore, it is suggested that aqueous extract OG can act via the same pathway as vitamin C, in maintaining the normal histological structures of the thymus of adult albino wistar rat exposed to lead toxicity.

Ameliorative effect of vitamin C on the haematological changes induced by exposure of chlorpyriphos and lead acetate in Wistar rats

2013 ◽  
Vol 23 (4) ◽  
pp. 829-834
Author(s):  
Nisar Ahmad Nisar ◽  
Mudasir Sultana ◽  
Naseer Ahmad Baba ◽  
Parveez Ahmad Para ◽  
Hina Ashraf Waiz ◽  
...  
Keyword(s):  
Vitamin C ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Ameliorative Effect ◽  
Haematological Changes

scholarly journals Ameliorative Effect of Trevo Dietary Supplement Against Lead Acetate Nephrotoxicity

2022 ◽  
Vol 16 (1) ◽  
pp. 34-42
Author(s):  
Omotayo B. Ilesanmi ◽  
◽  
Eni-yimini Solomon Agoro ◽  
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Antioxidant Properties ◽  
Kidney Tissue ◽  
Lead Toxicity ◽  
Serum Levels ◽  
Tissue Samples ◽  
Male Wistar Rats

Background: Trevo is a nutritional supplement with numerous bioactive natural products, with detoxifying and antioxidant properties. The purpose of this study was to investigate the ability of Trévo to protect against oxidative stress induced by lead in the kidneys of male Wistar rats. Methods: Thirty-five healthy male Wistar rats were divided into five groups of seven rats each, using a randomized design. I=control; II=15 mg/kg of lead acetate (PbA); III=2 ml/kg of trevo+PbA; IV=5 ml/kg of trevo+PbA; V=5 ml/kg of trevo. Animals were treated with trevo for five days before co-administration with lead intraperitoneally for 10 consecutive days. Animals were sacrificed 24hr after the last administration, blood samples were collected via cardiac puncture, and processed for assessment of urea, creatinine, and uric acid (UA), while the kidney samples were excised and processed for the following biochemical assays: Malondialdehyde (MDA), Glutathione-S-Transferase (GST), Catalase (CAT), Superoxide Dismutase (SOD), and Reduced Glutathione (GSH). Results: Injection of PbA caused a significant increase in the serum levels of urea, creatinine, and uric acid, and a significant increase (P<0.001) in the MDA concentration, and decreases in GSH concentration, CAT, SOD, and GST activities (P<0.05) as compared to the controls. Pretreatment with trevo prevented the oxidative stress induced by lead acetate in the kidney tissue samples and improve the renal function. The protective effect was evident at 5 ml/kg of trevo. Conclusion: The results showed that trevo was nephroprotective against lead toxicity and the activity might be linked to the presence of numerous antioxidant phytochemicals present in trevo.

scholarly journals Effect of aqueous extract of Ocimum gratissimum on acetaminophen induced renal toxicity in male wistar rats

2018 ◽  
Vol 12 (28) ◽  
pp. 522-527
Author(s):  
Linda Okerulu ◽  
Ani Celestine ◽  
Nworgu Choice ◽  
Uzoma Igwe ◽  
Emeka Ugwuishi ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Wistar Rats ◽  
Renal Toxicity ◽  
Ocimum Gratissimum ◽  
Male Wistar Rats

Ameliorative effect of aqueous extract of bitter leaf on egg yolk induced kidney toxicity in adult wistar rats

2017 ◽  
Vol 2 (2) ◽  
pp. 25
Author(s):  
Richard Agbonluai Ehimigbai
Keyword(s):  
Aqueous Extract ◽  
Wistar Rats ◽  
Egg Yolk ◽  
Wistar Rat ◽  
Normal Diet ◽  
Kidney Toxicity ◽  
Ameliorative Effect ◽  
Group A ◽  
Vascular Obstruction ◽  
Group B

ABSTRACT The aim of the study is to assess the effect of aqeueous extract of  bitter leaf on egg yolk induced kidney toxicity in adult wistar rat. A total of 44 (forty four) adult wistar rats weighing between 220 g to 250 g were separated into 4 groups of 11 rats per group. Group A rats were placed on normal diet only while Group B rats received 300 mg/ kg body weight / day (BWT/D) of egg yolk. Group C rats received 250mg / kg BWT/D of bitter leaf. Group D rats received 300 mg/ kg BWT/D of egg yolk and 250mg / kg BWT/D of bitter leaf. The dosage were given for 32 days via orogastric method. The biochemical outcome showed that group B revealed marked elevation in the urea, SOD, cholesterol level when compared with the other groups. Histologically, group B showed a mild vascular obstruction and patchy tubular necrosis, while group A, C and D revealed normal architecture of the kidney. We can therefore concluded that the aqueous extract of bitter leaf have antioxidative and neproprotective properties against egg yolk induced kidney toxicity in wistar rat.

scholarly journals Efek Pemberian Vitamin C terhadap Gambaran Histopatologi Hati Tikus Wistar yang Terpapar Timbal Asetat

2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Arifuddin Arifuddin ◽  
Aswiyanti Asri ◽  
Elmatris Elmatris
Keyword(s):  
Vitamin C ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Cell Injury ◽  
Wistar Rat ◽  
Pathology Laboratory ◽  
Liver Histopathology ◽  
Significant Difference ◽  
C 50 ◽  
One Way Anova

AbstrakPaparan timbal asetat dapat menyebabkan kerusakan sel hati dan menurunkan aktivitas enzim yang bekerja sebagai antioksidan esensial dalam tubuh. Vitamin C merupakan senyawa alami yang bersifat antioksidan kuat dan pengikat radikal bebas.Tujuan penelitian ini adalah menentukan efek pemberian vitamin C terhadap gambaran histopatologi hati tikus wistar yang terpapar plumbum asetat dan dilakukan di laboratorium Fakultas Farmasi dan laboratorium Patologi Anatomi Fakultas Kedokteran Universitas Andalas. Penelitian ini dilakukan pada 28 ekor tikus wistar jantan, berumur 3 bulan, berat badan ± 200 gr. Didapatkan bahwa pada pemaparan timbal 50 mg/kgBB/hari selama 4 minggu meningkatkan skor Manja Roenigk hati tikus wistar. Penambahan vitamin C 50 mg/kgBB/hari dan 75 mg/kgBB/hari pada tikus yang terpapar timbal asetat menunjukkan penurunan skor Manja Roenigk pada hati tikus wistar. Berdasarkan uji t berpasangan ternyata tidak terdapat perbedaan yang signifikan antara skor Manja Roenigk yang tidak terpapar dan terpapar timbal asetat (p = 0,501). Berdasarkan uji one way Anova terdapat pengaruh yang signifikan vitamin C terhadap gambaran histopatologi hati tikus wistar (p = 0,000). Secara statistik pemberian kedua dosis vitamin C ini tidak memberikan perbedaan yang bermakna (p = 0.379).Kesimpulan penelitian ini adalah terdapat pengaruh proteksi vitamin C terhadap mikroskopis hati tikus wistar yang telah terpapar timbal asetat.Kata kunci: Timbal Asetat, Gambaran Histopatologi Hati, Vitamin C, Plumbum Asetat, Manja Roenigk AbstractExposure of lead acetate can caused cell injury in liver and decrease activity of enzyme which works as essential antioxidant in body. Vitamin C is a natural compound as potent antioxidant and free radical binding.The objective of this study was to determine the effect of vitamin C on liver histopathology in exposed lead acetate wistar rats. This research was conducted at Pharmacy Laboratory and Pathology laboratory, Faculty of Medicine, Andalas University. The study performed on 28 male Wistar rats, 3 months old, body weight 200 g.             It was found that 50 mg/ kgBW/day of lead exposure for 4 weeks increased Manja Roenigk’s grading of wistar rat’s liver. Administration of vitamin C 50 mg/kgBW/day and 75 mg/kgBB/day to rat which exposed to lead acetate showed decrease in score of Manja Roenigk on wistar rat’s liver. Based on paired sample t test was not significant difference score of Manja Roenigk between unexposed and exposed lead acetate (p = 0.501). Based on one way Anova test was found  a significant effect of vitamin C to histopathology of wistar rat’s liver (p = 0.000). Statistically administration of both dose vitamin C did not give significant difference (p = 0.379).The conclusion of this study show  there is the effect of protection from administration of vitamin C to microscopic Wistar rat liver which exposed to lead acetateKeywords: lead acetate, histopathology of liver, vitamin C, plumbum acetate, Manja Roenigk

Adansonia digitata L. leaf extract attenuates lead-induced cortical histoarchitectural changes and oxidative stress in the prefrontal cortex of adult male Wistar rats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Vivian Atuadu ◽  
Ben-Azu Benneth ◽  
John Oyem ◽  
Emmanuel Esom ◽  
Chris Mba ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Prefrontal Cortex ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Neurological Diseases ◽  
Lead Toxicity ◽  
Adansonia Digitata ◽  
Male Wistar Rats ◽  
Aqueous Leaf Extract

AbstractObjectivesAdansonia digitata L. is popularly known for the management of various neurological diseases in ethno-medicine. Studies have shown that lead toxicity is a possible risk factor for early onset of neurodegenerative disease. Hence, this study was designed to evaluate the effect of A. digitata aqueous leaf extract (ADALE) against lead-induced oxidative stress and histo-architectural changes in the prefrontal cortex of adult Wistar rats.MethodsSaline (10 mL/kg), ADALE (500 and 1000 mg/kg) and EDTA (55 mg/kg) were pretreated orally 30 min prior to lead acetate (LA) (120 mg/kg) administration to male Wistar rats (n=7) for 21 days. Thereafter, standard biochemical (superoxide dismutate, catalase, glutathionxe and malondialdehyde), histological (H&E) and histochemical assessment (crystyl fast violet stain for nissil substance) were carried out in the prefrontal cortex.ResultsADALE significantly (p<0.05) reversed LA-induced oxidative stress, as evidenced by increased catalase, superoxide dismutase and oxidized glutathione levels, and decreased malondialdehyde concentration in the prefrontal cortex. Also, the increase chromatolysis and neuronal pyknosis of the pyramidal neurons of the prefrontal cortex were significantly attenuated by ADALE.ConclusionsThe result of this study showed that A. digitata aqueous leaf extract attenuated lead acetate-induced cortical neurodegeneration via inhibition of oxidative stress.

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