Potential of Microrhizomes for In Vitro Gingerol and Shogaol Synthesis in Ginger (Zingiber officinale Rosc.)

Author(s):  
Manjusha Rani ◽  
M. R. Shylaja ◽  
Deepu Mathew ◽  
D. Girija ◽  
M. Asha Shankar ◽  
...  
Keyword(s):  
Author(s):  
Lavinia dos Santos Mascarenhas ◽  
Monica Junqueira Machado ◽  
Noelma de Brito Miranda ◽  
Vânia de Jesus Santos Oliveira ◽  
Pedro Henrique Ribeiro Santana

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.


2021 ◽  
pp. 2100048
Author(s):  
Hou Mingda ◽  
Gao Jun ◽  
Liu Ziqi ◽  
Shan Yu ◽  
Liu Shuyong
Keyword(s):  

2016 ◽  
Vol 115 (7) ◽  
pp. 2637-2645 ◽  
Author(s):  
Ahmad K. Dyab ◽  
Doaa A. Yones ◽  
Zedan Z. Ibraheim ◽  
Tasneem M. Hassan

2018 ◽  
Vol 29 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Tânia Mara da Silva ◽  
Virgílio Vilas Boas Fernandes Junior ◽  
Rosemary Soares de Santana ◽  
Renata Marques de Melo Marinho ◽  
Márcia Carneiro Valera ◽  
...  

Abstract The aim of this study was to evaluate in vitro the influence of 20% glycolic ginger extract on the bond strength of glass-fiber post cemented with etch-and-rinse or self-etching resin cement. Forty-eight bovine roots were standardized (17±0.5 mm) and randomly divided into two groups, according to irrigant used during biomechanical preparation: NaOCl: 1% sodium hypochlorite and GEN: 20% glycolic ginger extract. Root canal was prepared and randomly assigned to one of two subgroups (n=12), according to luting protocol: self-etching (RelyX U200) and etch-and-rinse (RelyX ARC). After 48 h, the roots were sectioned perpendicularly (4 in cervical third and 3 in middle third) and submitted to push-out bond strength test (50 kgf load cell, 1.0 mm/min). Failure mode was analyzed in SEM and stereomicroscopy. Data were analyzed by three-way ANOVA and Tukey tests (p<0.05). ANOVA revealed that there were no significant differences between U200 (2.01±0.17)B and ARC (1.93±0.12)B in GEN group, and at the cervical third for both irrigants, 2.69±0.16A and 2.54±0.17A, respectively. Middle third showed lower bond strength than cervical third, regardless the irrigant. Overall, the cervical third regardless the irrigant employed and the association between NaOCl and ARC, presented better adhesive performance.


Author(s):  
Nadar Sowmya ◽  
Chouhan Raghavendra Singh ◽  
Kosha Patel ◽  
Harshil Patel ◽  
Tanvi Dodiya ◽  
...  

According to the literature survey most of the studies done on Adarak (Zingiber officinale rhizome) were performed on alcoholic extracts or isolated entities of ginger but no profound work has been done on the traditionally prepared or commonly consumed way of ginger. So, in this current study, fresh ginger rhizomes were traditionally pounded to make Adarak juice. The different concentration (0.05%, 0.5%, 1%, 2.5%, 5%, 10%) were taken as sample to perform onion root tip inhibition assay and brine shrimp lethality bioassay. It showed antimitotic inhibition in the range of 0.05-10% concentration with an IC50 value of 0.37 % on number of rootlets was 0.08 % on length of rootlets. The traditionally prepared Adarak juice showed cytotoxicity in the range of 0.05-2.5% concentration with LC50 value of 1.59%. The traditionally prepared Adarak juice possesses cytotoxic and antimitotic activity.


2021 ◽  
Vol 12 (1) ◽  
pp. 1-12
Author(s):  
Alexander Pérez Cordero ◽  
Leonardo Chamorro Anaya ◽  
Jorge Mercado Gomez

Los aceites esenciales son compuestos orgánicos importantes en el sector farmacéutico debido a sus propiedades antimicrobianas, volviéndose una alternativa de solución para determinadas fitopatologías. En el presente estudio se evaluó in vitro la actividad inhibitoria de aceites esenciales de Curcuma longa y Zingiber officinale contra Colletotrichum sp. obtenidas a partir de cultivos de Dioscorea rotundata en Toluviejo (Sucre, Colombia). Concentraciones de 3,000; 5,000; 8,000 y 10,000 mg/L de extracciones de aceite (método de hidrodestilación asistida por microondas) fueron aplicados a cultivos in vitro del hongo. Además, se empleó un testigo absoluto y controles positivo y negativo. Los aceites fueron caracterizados por cromatografía de gases acoplado a espectrometría de masa. La actividad inhibitoria se realizó por medio de la prueba de siembra en superficie (porcentaje de índice de inhibición). Los mayores valores de índice antifúngico se observaron a 10,000 mg/L similar al obtenido con el control positivo por benomil. De la caracterización química de los aceites esenciales el metabolito secundario que se presentó con mayor rango fue el monoterpeno 3-Thujeno y el citral. Los aceites esenciales de C. longa y Z. officinale generaron actividad inhibitoria contra el crecimiento de Colletotrichum sp., y por lo tanto pueden ser empleados para reducir la antracnosis.


Sign in / Sign up

Export Citation Format

Share Document