In-vitro and in vivo studies of traditionally prepared Zingiber officinale juice for Antimitotic and Lethality activity

2021 ◽  
pp. 4652-4656
Author(s):  
Nadar Sowmya ◽  
Chouhan Raghavendra Singh ◽  
Kosha Patel ◽  
Harshil Patel ◽  
Tanvi Dodiya ◽  
...  
Keyword(s):  
Zingiber Officinale ◽  
In Vivo Studies ◽  
Root Tip ◽  
Inhibition Assay ◽  
Lc50 Value ◽  
Antimitotic Activity ◽  
Ic50 Value ◽  
Traditionally Prepared

According to the literature survey most of the studies done on Adarak (Zingiber officinale rhizome) were performed on alcoholic extracts or isolated entities of ginger but no profound work has been done on the traditionally prepared or commonly consumed way of ginger. So, in this current study, fresh ginger rhizomes were traditionally pounded to make Adarak juice. The different concentration (0.05%, 0.5%, 1%, 2.5%, 5%, 10%) were taken as sample to perform onion root tip inhibition assay and brine shrimp lethality bioassay. It showed antimitotic inhibition in the range of 0.05-10% concentration with an IC50 value of 0.37 % on number of rootlets was 0.08 % on length of rootlets. The traditionally prepared Adarak juice showed cytotoxicity in the range of 0.05-2.5% concentration with LC50 value of 1.59%. The traditionally prepared Adarak juice possesses cytotoxic and antimitotic activity.

scholarly journals Antidiabetic‎ Activity of Terfezia Claveryi; An In Vitro and In Vivo Study

2019 ◽  
Vol 12 (2) ◽  
pp. 603-608
Author(s):  
Anas AlAhmed ◽  
Hany Ezzat Khalil
Keyword(s):  
Methanol Extract ◽  
Positive Control ◽  
Alpha Tocopherol ◽  
Antidiabetic Activity ◽  
Inhibition Assay ◽  
Ic50 Value ◽  
Enzymatic Glycosylation ◽  
Terfezia Claveryi

The main objective of current study was to investigate the in vitro and in vivo antidiabetic activity of Terfezia claveryi methanol extract. In vitro antidiabetic assays such as inhibition of α-amylase enzyme and non-enzymatic glycosylation of hemoglobin were carried out. The results of α- amylase inhibition assay revealed that the inhibitory activity (IC50) of Terfezia claveryi methanol extract (‎38.7µg/ml) is stronger when compared with positive control (Acarbose IC50 value of ‎45.3‎ µg/ml). The inhibition of glycosylation of hemoglobin of Terfezia claveryi methanol extract showed almost the same IC50 (33.1µg/ml) when compared the positive control, alpha-tocopherol (‎35.4µg/ml‎). In vivo antidiabetic study revealed that Terfezia claveryi methanol extract ‎ possessed good activity at a dose of 200 mg/kg through reducing the fasting plasma glucose level (122.1‎±‎3.0 mg/dl) when compared with positive control (Glibenclamide of ‎79.4±1.4‎ mg/dl) (p < 0.001). The results from this study indicated that Terfezia claveryi methanol extract exhibited considerable in vitro and in vivo antidiabetic activities. These possible activities could be useful to consider Terfezia claveryi ‎ as therapeutic antidiabetic candidate.

scholarly journals In vitro and in vivo studies of anti diabetic effect of khaya senegalensis leaves and bark extracts

2019 ◽  
Vol 7 (1) ◽  
pp. 12
Author(s):  
Marvit Osman Widdat Allah ◽  
Ayat Ahmed Alrasheid ◽  
Eltayeb Suliman Elamin
Keyword(s):  
Diabetes Mellitus ◽  
In Vitro Models ◽  
In Vivo Studies ◽  
Health Concern ◽  
Bark Extract ◽  
Diabetes Model ◽  
Ic50 Value ◽  
Khaya Senegalensis

Diabetes mellitus in Sudan is one of public health concern since it causes significant mortality and complications for long term. Though conventional drugs are used in the management of diabetes mellitus they are expensive, unavailable and also have numerous side effects. Khaya senegalensis has traditionally used in the management of diabetes. The present study was conducted to examine the In vitro and In vivo anti-diabetic activity of leaves and bark extracts of Khaya senegalensis. The leaves and bark of the plant were extracted with ethanol 96%, and then tested for anti-diabetic activity in a series of in vitro models and a type 2 diabetes model of rats. In vitro bark extract of k.senegalensis showed higher inhibitory activities against the enzyme with IC50 value 226.14 µg/ml. In vivo oral administration of the extracts of the k. senegalensis exhibited decrease in blood sugar level and was found to be time dependent. Bark extract showed strong in vitro and in vivo anti diabetic activity.  

scholarly journals Composition and Comprehensive Antioxidant Activity of Ginger (Zingiber officinale) Essential Oil from Ecuador

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Martina Höferl ◽  
Ivanka Stoilova ◽  
Juergen Wanner ◽  
Erich Schmidt ◽  
Leopold Jirovetz ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Zingiber Officinale ◽  
Yeast Cells ◽  
In Vivo Studies ◽  
Oxidase Inhibition ◽  
Xanthine Oxidase Inhibition ◽  
Lipid Peroxidation Inhibition

In the present study, the chemical composition and antioxidant potential of an essential oil of ginger rhizomes from Ecuador was elucidated. The analysis of the essential oil by GC/FID/MS resulted in identification of 71 compounds, of which the main are citral (geranial 10.5% and neral 9.1%), α-zingiberene (17.4%), camphene (7.8%), α-farnesene (6.8%) and β-sesquiphellandrene (6.7%). The in vitro antioxidant activity of the essential oil expressed by IC50 in descending order is: hydroxyl radical (OH•) scavenging (0.0065 μg/mL) > chelating capacity (0.822 μg/mL) > 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical cation (ABTS•+) scavenging (3.94 μg/mL) > xanthine oxidase inhibition (138.0 μg/mL) > oxygen radical (CV) scavenging (404.0 μg/mL) > 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) scavenging (675 μg/mL). Lipid peroxidation inhibition of the essential oil was less efficient than butylhydroxy-toluol (BHT) in both stages, i.e. hydroperoxide and malondialdehyde formation. In vivo studies in Saccharomyces cerevisiae demonstrated a significant dose-dependent increase in antioxidant marker enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), blocking the oxidation processes in yeast cells. Moreover, ginger essential oil in concentrations of 1.6 mg/mL increases the viability of cells to oxidative stress induced by H2O2.

Imbir lekarski ? roślina o właściwościach neuroochronnych

2019 ◽  
Vol 20 (4) ◽  
Author(s):  
Dominika Łażewska ◽  
Kinga Miętkiewska ◽  
Elżbieta Studzińska-Sroka
Keyword(s):  
Neurodegenerative Disorders ◽  
Chemical Compounds ◽  
Zingiber Officinale ◽  
Raw Material ◽  
In Vivo Studies ◽  
Neuroprotective Activity ◽  
Subtropical Zone ◽  
Loss Of Appetite

Ginger (Zingiber officinale Rosc.) is a perennial belonging to Zingiberaceae family. Primary place of occurrence was Asia, but nowadays it is grown in many regions of subtropical zone. The raw material used in medicine is a rhizome and oil obtained from it. Chemical compounds responsible for pharmacological activity are mainly sesquiterpene alcohols, gingerols and shogaols. Among beneficial properties of ginger we can list: promoting salivation, stimulating secretion of gastric juice and bile, reducing cholesterol level, improving immunity, activating peristalsis of the intestines and antioxidative, antibacterial, antiviral, anticoagulant and anti-inflammatory properties. Confirmed indications are loss of appetite, dyspepsia and motion sickness. In this paper we described scientific reports considering potential neuroprotective activity of ginger, which can be used in treatment of neurodegenerative disorders as Alzheimer’s disease. We presented the results of in vitro and in vivo studies. The article contains information on the safety of products from Z. officinale.

Anti-Cancer Effect of Gingerol in Cancer Prevention and Treatment

2020 ◽  
Vol 20 ◽  
Author(s):  
Sana Nafees ◽  
Md. Zafaryab ◽  
Syed H. Mehdi ◽  
Bushra Zia ◽  
Moshahid A. Rizvi ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Zingiber Officinale ◽  
Basic Mechanism ◽  
In Vivo Studies ◽  
Cancer Therapies ◽  
Beneficial Effects ◽  
Proinflammatory Mediators ◽  
Natural Agents

: Cancer is one of the most lethal diseases in the world. Because of high death rate associated with cancer and the side effects of chemotherapy and radiation therapy, patients required alternative strategies for its treatment. Ginger (Zingiber officinale) has an enormous medicinal properties and health beneficial effects. In this review, we discuss the basic mechanism by which gingerol (a active component of ginger) modulate a variety of cell signaling pathways linked to cancer, including nuclear factors (NF-κB), signal transducer and activator of transcription 3 (STAT3), activator protein1 (AP-1), β- catenin, growth factors receptors (EGFR, VEGFR); mitogen activated protein kinases (MAPK) and proinflammatory mediators (TNF-α and COX-2). Both in vitro and in vivo studies support the role of gingerol in cancer. The efficacy of gingerol by clinical trials has also been reported. Importantly, natural agents are already in clinical trials against various kinds of cancer. Effort has been made through this comprehensive review to highlight the recent developments and milestones achieved in cancer therapies via studies based on different cell lines using gingerol.

scholarly journals Some Common Medicinal Plants with Antidiabetic Activity, Known and Available in Europe (A Mini-Review)

2022 ◽  
Vol 15 (1) ◽  
pp. 65
Author(s):  
Monika Przeor
Keyword(s):  
Raw Materials ◽  
Antioxidant Properties ◽  
Zingiber Officinale ◽  
Biologically Active ◽  
In Vivo Studies ◽  
Antidiabetic Activity ◽  
Plant Materials ◽  
Trigonella Foenum Graecum L

Diabetes is a metabolic disease that affected 9.3% of adults worldwide in 2019. Its co-occurrence is suspected to increase mortality from COVID-19. The treatment of diabetes is mainly based on the long-term use of pharmacological agents, often expensive and causing unpleasant side effects. There is an alarming increase in the number of pharmaceuticals taken in Europe. The aim of this paper is to concisely collect information concerning the few antidiabetic or hypoglycaemic raw plant materials that are present in the consciousness of Europeans and relatively easily accessible to them on the market and sometimes even grown on European plantations. The following raw materials are discussed in this mini-review: Morus alba L., Cinnamomum zeylanicum J.Presl, Trigonella foenum-graecum L., Phaseolus vulgaris L., Zingiber officinale Rosc., and Panax ginseng C.A.Meyer in terms of scientifically tested antidiabetic activity and the presence of characteristic biologically active compounds and their specific properties, including antioxidant properties. The characteristics of these raw materials are based on in vitro as well as in vivo studies: on animals and in clinical studies. In addition, for each plant, the possibility to use certain morphological elements in the light of EFSA legislation is given.

In vitro and in vivo studies on the bioactivity of a ginger (Zingiber officinale) extract towards adult schistosomes and their egg production

2002 ◽  
Vol 76 (3) ◽  
pp. 241-247 ◽  
Author(s):  
L. Sanderson ◽  
A. Bartlett ◽  
P.J. Whitfield
Keyword(s):  
Egg Production ◽  
Zingiber Officinale ◽  
In Vivo Studies ◽  
Separate Experiment ◽  
Laboratory Mice ◽  
Significant Difference ◽  
Almost All ◽  
And Control

AbstractThe bioactivity of an ethyl acetate extract of ginger (Zingiber officinale) towards Schistosoma mansoni adult pairs, both cultured in vitro and in vivo in laboratory mice, was investigated by monitoring worm mortality and fecundity. In vitro, a concentration of 200 mg l-1 of extract killed almost all worms within 24 h. Male worms seemed more susceptible than female under these conditions. Cumulative egg output of surviving worm pairs in vitro was considerably reduced when exposed to the extract. For example, after 4 days of exposure to 50 mg l-1, cumulative egg output was only 0.38 eggs per worm pair compared with 36.35 for untreated worms. In vivo efficacy of the extract was tested by oral and subcutaneous delivery of 150 mg kg-1 followed by assessment of worm survival and fecundity. Neither delivery route produced any significant reduction in worm numbers compared with untreated controls. Worm fecundity was assessed in vivo by cumulative egg counts per liver at 55 days post infection with mice treated subcutaneously. Such infections showed egg levels in the liver of about 2000 eggs per worm pair in 55 days, in both treated and control mice, with no significant difference between the two groups. To ensure that density-dependent effects did not confound this analysis, a separate experiment demonstrated no such influence on egg output per worm pair, at intensities between 1 and 23 worms per mouse.

scholarly journals EFFECT OF TRIGONELLA FOENUM GRAECUM ON ΑLPHA-GLUCOSIDASE AND DIPEPTIDYL PEPTIDASE-IV INHIBITORY ACTIVITY - AN IN VITRO STUDY

2018 ◽  
Vol 11 (10) ◽  
pp. 513
Author(s):  
Inbaraj Sd ◽  
Muniappan M
Keyword(s):  
Inhibitory Activity ◽  
Dipeptidyl Peptidase ◽  
Seed Extract ◽  
Inhibition Assay ◽  
Dipeptidyl Peptidase 4 ◽  
Trigonella Foenum Graecum ◽  
Ic50 Value ◽  
Alpha Glucosidase

Objective: The objective of this study is to find the effect of seed extract of Trigonella foenum graecum on the inhibition of α-glucosidase and dipeptidyl peptidase-4 (DPP-4) enzyme activity by in vitro method.Methods: Methanolic seed extract of T. foenum graecum seed was prepared and supplied by Sami Labs, Bengaluru, on request. For alpha-glucosidase inhibition assay, the following concentrations (0, 20, 40, 60, 80, and 100 μg/ml) of extracts and for DPP-4 inhibition assay (0, 5, 10, 20, 40, 80, 160, and 320d μg/ml) concentrations were used. The absorbance was measured at 540 and 405 nm using multiplate reader, and the percentage of α-glucosidase and DPP-4 enzyme inhibitory activity of extract fractions was calculated. Acarbose for alpha-glucosidase inhibition and vildagliptin for DDP-4 inhibition were used as standard drugs. The IC50 value for alpha-glucosidase inhibition and DPP-4 inhibition was determined.Results: The maximum alpha-glucosidase inhibitory activity of T. foenum graecum extract at 100 μg/ml was 68% (p<0.05) with IC50 value of 57.25 when compared to the acarbose (STD) of 94% with IC50 values of 42.78. The maximum percentage of DPP-4 inhibition of T. foenum graecum extract at 320 μg/ml is 77.84% (p<0.01) with IC50 value of 52.26 when compared to the vildagliptin (STD) it is 80.15% with IC50 value of 22.98.Conclusion: The results of the in vitro studies show that T. foenum graecum seed extract has significant alpha-glucosidase and DPP-4 inhibition. Further in vivo and clinical studies are necessary to establish the antihyperglycemic and antidiabetic potential of T. foenum graecum seed extract for the treatment of Type 2 diabetes mellitus.

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