Neuroprotective effect of Bauhinia variegata Linn. leaf extracts in streptozotocin induced diabetes in Sprague Dawley rats

2021 ◽  
Author(s):  
Ankit P. Laddha ◽  
Mayuresh S. Garud ◽  
Yogesh A. Kulkarni
Keyword(s):  
Neuroprotective Effect ◽  
Sprague Dawley ◽  
Leaf Extracts ◽  
Sprague Dawley Rats ◽  
Streptozotocin Induced Diabetes ◽  
Bauhinia Variegata

Curcumin Reduces Neuroinflammation and Improves the Impairments of Anesthetics on Learning and Memory by Regulating the Expression of miR-181a-5p

2021 ◽  
pp. 1-9
Author(s):  
Guizhen Liu ◽  
Yuchuan Sun ◽  
Fei Liu
Keyword(s):  
Cognitive Impairment ◽  
Protective Effect ◽  
Learning And Memory ◽  
Cognitive Dysfunction ◽  
Inflammatory Cytokines ◽  
Neuroprotective Effect ◽  
Morris Water Maze Test ◽  
Sprague Dawley ◽  
Protein Levels ◽  
Sprague Dawley Rats

<b><i>Objective:</i></b> The purpose of this study was to explore the role of curcumin (Cur) in isoflurane (ISO)-induced learning and memory dysfunction in Sprague-Dawley rats and further elucidate the mechanism of the protective effect produced by Cur. <b><i>Methods:</i></b> Rat models of cognitive impairment were established by inhaling 3% ISO. The Morris water maze test was used to assess the cognitive function of rats. ELISA and qRT-PCR were used to analyze the protein levels of pro-inflammatory cytokines and expression levels of miR-181a-5p, respectively. <b><i>Results:</i></b> Cur significantly improved the ISO-induced cognitive dysfunction in rats and alleviated the ISO-induced neuroinflammation. miR-181a-5p was overexpressed in ISO-induced rats, while Cur treatment significantly reduced the expression of miR-181a-5p. Overexpression of miR-181a-5p promoted the cognitive impairment and the release of inflammatory cytokines and reversed the neuroprotective effect of Cur. <b><i>Conclusion:</i></b> Cur has a protective effect on ISO-induced cognitive dysfunction, which may be achieved by regulating the expression of miR-181a-5p.

scholarly journals Effect of Catha edulis (khat) on pancreatic functions in streptozotocin-induced diabetes in male Sprague-Dawley rats

2018 ◽  
Vol 67 (4) ◽  
pp. 517-526 ◽  
Author(s):  
Abdulsamad Alsalahi ◽  
Mohammed A. Alshawsh ◽  
Zamri Chik ◽  
Zahurin Mohamed
Keyword(s):  
Sprague Dawley ◽  
Catha Edulis ◽  
Sprague Dawley Rats ◽  
Streptozotocin Induced Diabetes

scholarly journals Effect of Ranirestat, a New Aldose Reductase Inhibitor, on Diabetic Retinopathy in SDT Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Fumihiko Toyoda ◽  
Yoshiaki Tanaka ◽  
Ayumi Ota ◽  
Machiko Shimmura ◽  
Nozomi Kinoshita ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Aldose Reductase ◽  
Reductase Inhibitor ◽  
Retinal Thickness ◽  
Neuroprotective Effect ◽  
Treated Group ◽  
Aldose Reductase Inhibitor ◽  
Untreated Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

Purpose. To evaluate the effect of ranirestat, a new aldose reductase inhibitor (ARI), on diabetic retinopathy (DR) in Spontaneously Diabetic Torii (SDT) rats.Methods. The animals were divided into six groups, normal Sprague-Dawley rats(n=8), untreated SDT rats(n=9), ranirestat-treated SDT rats (0.1, 1.0, and 10 mg/kg/day,n=7, 8, and 6, resp.), and epalrestat-treated SDT rats (100 mg/kg/day,n=7). Treated rats received oral ranirestat or epalrestat once daily for 40 weeks after the onset of diabetes. After the eyes were enucleated, the retinal thickness and the area of stained glial fibrillary acidic protein (GFAP) were measured.Results. The retinas in the untreated group were significantly thicker than those in the normal and ranirestat-treated (0.1, 1.0, and 10 mg/kg/day) groups. The immunostained area of GFAP in the untreated group was significantly larger than that in the normal and ranirestat-treated (1.0 and 10 mg/kg/day) groups. There were no significant differences between the untreated group and epalrestat-treated group in the retinal thickness and the area of stained GFAP.Conclusion. Ranirestat reduced the retinal thickness and the area of stained GFAP in SDT rats and might suppress DR and have a neuroprotective effect on diabetic retinas.

Effect of Streptozotocin-Induced Diabetes on Estradiol-Stimulated Changes in the Ultrastructure of the Rat Endometrium

1981 ◽  
Vol 39 ◽  
pp. 532-533
Author(s):  
G.T. Frederick ◽  
R.M. Gardner ◽  
J.M. Kirkland ◽  
G.M. Stancel
Keyword(s):  
Electron Microscopic Examination ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Whole Cells ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sprague Dawley Rats ◽  
Uterine Growth ◽  
Streptozotocin Induced Diabetes

Estradiol (E2)-stimulated uterine growth has been well characterized both biochemically and morphologically. Recent studies have shown that insulin plays an important role in the regulation of cellular development. This study examines the effect of streptozotocin-induced diabetes on E2-stimulated changes in the ultrastructure of the endometrium of the rat uterus.Sprague-Dawley rats were ovariectomized at 21 days of age. Diabetes, defined as blood glucose levels greater than 300mg%, was induced in half of these animals by the injection of 85mg streptozotocin/kg body weight.The remaining animals were classified as normal. Animals from both groups were injected with either 0.9% saline or 4μg E2/100gm body weight. At 18, 24, 36 and 48 hours post-injection of E2, uterine segments were collected from 8-10 animals in each experimental group and processed for electron microscopic examination. Uterine segments from saline-injected animals served as controls. Data summarized describe observations made from thin-sections and have not been extrapolated for whole cells.

Hyaluronic acid scaffold has a neuroprotective effect in hemisection spinal cord injury

2016 ◽  
Vol 25 (1) ◽  
pp. 114-124 ◽  
Author(s):  
Sergiy V. Kushchayev ◽  
Morgan B. Giers ◽  
Doris Hom Eng ◽  
Nikolay L. Martirosyan ◽  
Jennifer M. Eschbacher ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Neuroprotective Effect ◽  
Scar Tissue ◽  
Treated Group ◽  
Secondary Injury ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Cord Injury ◽  
Behavioral Assessments

OBJECTIVE Spinal cord injury occurs in 2 phases. The initial trauma is followed by inflammation that leads to fibrous scar tissue, glial scarring, and cavity formation. Scarring causes further axon death around and above the injury. A reduction in secondary injury could lead to functional improvement. In this study, hyaluronic acid (HA) hydrogels were implanted into the gap formed in the hemisected spinal cord of Sprague-Dawley rats in an attempt to attenuate damage and regenerate tissue. METHODS A T-10 hemisection spinal cord injury was created in adult male Sprague-Dawley rats; the rats were assigned to a sham, control (phosphate-buffered saline), or HA hydrogel–treated group. One cohort of 23 animals was followed for 12 weeks and underwent weekly behavioral assessments. At 12 weeks, retrograde tracing was performed by injecting Fluoro-Gold in the left L-2 gray matter. At 14 weeks, the animals were killed. The volume of the lesion and the number of cells labeled from retrograde tracing were calculated. Animals in a separate cohort were killed at 8 or 16 weeks and perfused for immunohistochemical analysis and transmission electron microscopy. Samples were stained using H & E, neurofilament stain (neurons and axons), silver stain (disrupted axons), glial fibrillary acidic protein stain (astrocytes), and Iba1 stain (mononuclear cells). RESULTS The lesions were significantly smaller in size and there were more retrograde-labeled cells in the red nuclei of the HA hydrogel–treated rats than in those of the controls; however, the behavioral assessments revealed no differences between the groups. The immunohistochemical analyses revealed decreased fibrous scarring and increased retention of organized intact axonal tissue in the HA hydrogel–treated group. There was a decreased presence of inflammatory cells in the HA hydrogel–treated group. No axonal or neuronal regeneration was observed. CONCLUSIONS The results of these experiments show that HA hydrogel had a neuroprotective effect on the spinal cord by decreasing the magnitude of secondary injury after a lacerating spinal cord injury. Although regeneration and behavioral improvement were not observed, the reduction in disorganized scar tissue and the retention of neurons near and above the lesion are important for future regenerative efforts. In addition, this gel would be useful as the base substrate in the development of a more complex scaffold.

scholarly journals Streptozotocin-induced diabetes impairs Mg2+ homeostasis and uptake in rat liver cells

2004 ◽  
Vol 286 (2) ◽  
pp. E184-E193 ◽  
Author(s):  
Theresa E. Fagan ◽  
Christie Cefaratti ◽  
Andrea Romani
Keyword(s):  
Liver Cells ◽  
Atp Content ◽  
Sprague Dawley ◽  
Uptake Mechanism ◽  
Diabetes Onset ◽  
Kinase C ◽  
Sprague Dawley Rats ◽  
Rat Liver Cells ◽  
Streptozotocin Induced Diabetes ◽  
Stimulation Of

Male Sprague-Dawley rats rendered diabetic by streptozotocin injection presented 10 and 20% decreases in total hepatic Mg2+ content at 4 and 8 wk, respectively, following diabetes onset. This decrease was associated with a parallel decrease in K+ and ATP content and an increase in Na+ level. In diabetic liver cells, the Mg2+ extrusion elicited by α1-adrenoceptor stimulation was markedly reduced compared with nondiabetic livers, whereas that induced by β-adrenoceptor stimulation was unaffected. In addition, diabetic hepatocytes did not accumulate Mg2+ following stimulation of protein kinase C pathway by vasopressin, diacylglycerol analogs, or phorbol 12-myristate 13-acetate derivates despite the reduced basal content in cellular Mg2+. Experiments performed in purified plasma membrane from diabetic livers located the defect at the level of the bidirectional Na+/Mg2+ exchanger operating in the basolateral domain of the hepatocyte cell membrane, which could extrude but not accumulate Mg2+ in exchange for Na+. The impairment of Mg2+ uptake mechanism, in addition to the decrease in cellular ATP level, can contribute to explaining the decrease in liver Mg2+ content observed under diabetic conditions.

scholarly journals Neuroprotective Effect of Gui Zhi (Ramulus Cinnamomi) on Ma Huang- (Herb Ephedra-) Induced Toxicity in Rats Treated with a Ma Huang-Gui Zhi Herb Pair

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Fang-hao Zheng ◽  
Ping Wei ◽  
Hui-ling Huo ◽  
Xue-feng Xing ◽  
Fei-long Chen ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Oxidative Damage ◽  
Neuroprotective Effect ◽  
Inhibitory Effect ◽  
Chinese Herbs ◽  
Clinical Use ◽  
Sprague Dawley ◽  
Neuroprotective Effects ◽  
Sprague Dawley Rats ◽  
Ma Huang

Herb Ephedra(Ma Huang in Chinese) andRamulus Cinnamomi(Gui Zhi in Chinese) are traditional Chinese herbs, often used together to treat asthma, nose and lung congestion, and fever with anhidrosis. Due to the adverse effects of ephedrine, clinical use of Ma Huang is restricted. However, Gui Zhi extract has been reported to decrease spontaneous activity in rats and exert anti-inflammatory and neuroprotective effects. The present study explored the possible inhibitory effect of Gui Zhi on Ma Huang-induced neurotoxicity in rats when the two herbs were used in combination. All Ma Huang and Ma Huang-Gui Zhi herb pair extracts were prepared using methods of traditional Chinese medicine and were normalized based on the ephedrine content. Two-month-old male Sprague-Dawley rats (n=6rats/group) were administered Ma Huang or the Ma Huang-Gui Zhi herb pair extracts for 7 days (ephedrine = 48 mg/kg), and locomotor activity was measured. After 7 days, oxidative damage in the prefrontal cortex was measured. Gui Zhi decreased hyperactivity and sensitization produced by repeated Ma Huang administration and attenuated oxidative stress induced by Ma Huang. The results of this study demonstrate the neuroprotective potential of Gui Zhi in Ma Huang-induced hyperactivity and oxidative damage in the prefrontal cortex of rats when used in combination.

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