scholarly journals The effect of Propolis on inhibition of Aspergillus parasiticus growth, aflatoxin production and expression of aflatoxin biosynthesis pathway genes

2020 ◽  
Vol 18 (1) ◽  
pp. 297-302 ◽  
Author(s):  
Hamideh Mahmoodzadeh Hosseini ◽  
Siavash Hamzeh Pour ◽  
Jafar Amani ◽  
Sima Jabbarzadeh ◽  
Mostafa Hosseinabadi ◽  
...  
Keyword(s):  
Aspergillus Parasiticus ◽  
Aflatoxin Production ◽  
Biosynthesis Pathway ◽  
Aflatoxin Biosynthesis

scholarly journals Analysis of impact of anaerobic condition on the aflatoxin production in Aspergillus parasiticus Speare

2019 ◽  
Vol 39 (1) ◽  
Author(s):  
Maneesh Department of Biotechnology, Mag Kumar ◽  
Harish Kumar ◽  
Roshan Kamal Topno ◽  
Jainendra Kumar
Keyword(s):  
High Performance ◽  
Aspergillus Parasiticus ◽  
Quantitative Estimation ◽  
Fungal Growth ◽  
Aflatoxin Production ◽  
Agricultural Products ◽  
Aflatoxin Biosynthesis ◽  
Anaerobic Conditions ◽  
Toxic Contamination ◽  
Aerobic And Anaerobic

Aflatoxins are the natural carcinogens that are the best characterized as fungal secondary metabolites. The producers that are responsible for aflatoxin biosynthesis are strongly associated in toxic contamination of essential agricultural products. Aspergillus parasiticus is an exclusive fungus that participates in causing hepatic problems in humans and cattle. These mycotoxins are greatly influenced by abiotic stresses. The fungal growth, proliferation and its toxigenicity are highly influenced by these stresses. Present study aimed to restrict the mycelial growth and to prevent aflatoxin preparation in A. parasiticus under the anoxic stress. The monosporic strains of A. parasiticus were grown in two different Erlenmeyer conical flasks containing Czapek Dox Broth and Czapek Dox Agar under both aerobic and anaerobic conditions. The anoxic condition was maintained using Anaero Bag System. Aflatoxin was isolated after 10 days, and quantitative estimation was done by using High Performance Liquid Chromatography (HPLC). The experimental outcome showed that there was a drastic decrease in both the morphological growth and the aflatoxin biosynthesis of A. parasiticus in anoxic state.

Production of Aflatoxin in Cocoa Beans

1979 ◽  
Vol 62 (5) ◽  
pp. 1076-1079 ◽  
Author(s):  
Lawrence M Lenovich ◽  
W Jeffrey Hurst
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Ivory Coast ◽  
Aspergillus Parasiticus ◽  
Aflatoxin Production ◽  
Growth Conditions ◽  
Aflatoxin Contamination ◽  
Cocoa Beans ◽  
Total Aflatoxin ◽  
Substrate Variation

Abstract Aflatoxin was produced in both non-autoclaved and autoclaved Ivory Coast cocoa beans inoculated with Aspergillus parasiticus NRRL 2999 under optimum laboratory growth conditions. Total aflatoxin levels ranged from 213 to 5597 ng/g substrate. Aflatoxin was quantitated by using high pressure liquid chromatography (HPLC). Raw, non-autoclaved cocoa beans, also inoculated with aspergilli, produced 6359 ng aflatoxin/g substrate. Variation in aflatoxin production between bean varieties was observed. Total aflatoxin levels of 10,446 and 23,076 ng/g substrate were obtained on Ivory Coast beans inoculated with A. parasiticus NRRL 2999 and NRRL 3240, respectively. Aflatoxin production on Trinidad and Malaysian beans was 28 and 65 ng aflatoxin/g substrate. These data support previously reported low level natural aflatoxin contamination in cocoa.

Antioxidant enzymes stimulation in Aspergillus parasiticus by Lentinula edodes inhibits aflatoxin production

2005 ◽  
Vol 69 (2) ◽  
pp. 207-215 ◽  
Author(s):  
M. Reverberi ◽  
A. A. Fabbri ◽  
S. Zjalic ◽  
A. Ricelli ◽  
F. Punelli ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Lentinula Edodes ◽  
Aspergillus Parasiticus ◽  
Aflatoxin Production

Extracts of Agave americana inhibit aflatoxin production in Aspergillus parasiticus

2011 ◽  
Vol 4 (1) ◽  
pp. 37-42 ◽  
Author(s):  
A. Rosas-Taraco ◽  
E. Sanchez ◽  
S. García ◽  
N. Heredia ◽  
D. Bhatnagar
Keyword(s):  
Aspergillus Parasiticus ◽  
Public Awareness ◽  
Aflatoxin Production ◽  
Aflatoxin Contamination ◽  
Aqueous Extracts ◽  
Total Aflatoxin ◽  
Toxigenic Fungi ◽  
Agave Americana ◽  
Acid Accumulation ◽  
Norsolorinic Acid

Toxigenic fungi invade crops prior to harvest as well as during storage and produce harmful, even carcinogenic toxins such as aflatoxins. Since consumers demand safe commodities, and due to enhanced public awareness of the dangers of many synthetic fungicides, the importance of investigating alternative, natural products to control these toxigenic fungi is clear. This study investigated the effect of aqueous extracts of Agave americana on growth, conidia and aflatoxin production. Aspergillus parasiticus strains SRRC 148, SRRC 143 (Su-1), and A. parasiticus SRRC 162, a mutant (nor-) that accumulates norsolorinic acid (NOR, an orange-coloured intermediate of the aflatoxin pathway), were first inoculated into Adye and Mateles liquid medium, then plant extracts were added, and incubated at 28 °C for 7 days. Aflatoxin and norsolorinic acid were assayed by HPLC and spectrophotometry, respectively. While the extract of A. americana stimulated growth of the studied fungi, conidiogenesis, norsolorinic acid accumulation (in the nor- mutant), and aflatoxin production were significantly affected. The reduction was produced by the extracts at concentrations higher than 5-10 mg/ml, where all types of total aflatoxin analysed (aflatoxins B1, B2, G1 and G2) were reduced from 64% to >99% in the whole culture, and a reduction of 75% of norsolorinic acid. The results of the present work indicate that extracts of A. americana may be promising safe alternatives to harmful fungicides for controlling aflatoxin contamination.

Effect of selected inhibitors on growth, pigmentation, and aflatoxin production by Aspergillus parasiticus

1986 ◽  
Vol 94 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Raul Valcarcel ◽  
J. W. Bennett ◽  
Joanne Vitanza
Keyword(s):  
Aspergillus Parasiticus ◽  
Aflatoxin Production

ChemInform Abstract: Blasticidin A as an Inhibitor of Aflatoxin Production by Aspergillus parasiticus

ChemInform ◽  
2001 ◽  
Vol 32 (15) ◽  
pp. no-no
Author(s):  
Shohei Sakuda ◽  
Makoto Ono ◽  
Hiroyuki Ikeda ◽  
Takefumi Nakamura ◽  
Yasuhito Inagaki ◽  
...  
Keyword(s):  
Aspergillus Parasiticus ◽  
Aflatoxin Production
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