Cell membrane stability and biochemical response of seven wheat cultivars under salinity stress

2015 ◽  
Vol 38 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Sepideh Sadat Jamali ◽  
Azam Borzouei ◽  
Mustafa Aghamirzaei ◽  
Hamid Reza Khosronejad ◽  
Milad Fathi
1991 ◽  
Vol 42 (2) ◽  
pp. 167-171 ◽  
Author(s):  
G. S. PREMACHANDRA ◽  
HIROHUMI SANEOKA ◽  
MUNEAKI KANAYA ◽  
SHOITSU OGATA

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Sung-Chih Hsieh ◽  
Jeng-Ting Tsao ◽  
Wei-Zhen Lew ◽  
Ya-Hui Chan ◽  
Lin-Wen Lee ◽  
...  

One of the causes of dental pulpitis is lipopolysaccharide- (LPS-) induced inflammatory response. Following pulp tissue inflammation, odontoblasts, dental pulp cells (DPCs), and dental pulp stem cells (DPSCs) will activate and repair damaged tissue to maintain homeostasis. However, when LPS infection is too serious, dental repair is impossible and disease may progress to irreversible pulpitis. Therefore, the aim of this study was to examine whether static magnetic field (SMF) can attenuate inflammatory response of dental pulp cells challenged with LPS. In methodology, dental pulp cells were isolated from extracted teeth. The population of DPSCs in the cultured DPCs was identified by phenotypes and multilineage differentiation. The effects of 0.4 T SMF on DPCs were observed through MTT assay and fluorescent anisotropy assay. Our results showed that the SMF exposure had no effect on surface markers or multilineage differentiation capability. However, SMF exposure increases cell viability by 15%. In addition, SMF increased cell membrane rigidity which is directly related to higher fluorescent anisotropy. In the LPS-challenged condition, DPCs treated with SMF demonstrated a higher tolerance to LPS-induced inflammatory response when compared to untreated controls. According to these results, we suggest that 0.4 T SMF attenuates LPS-induced inflammatory response to DPCs by changing cell membrane stability.


Author(s):  
Jun Yin ◽  
Yong Huang

Process-induced damage to cells is of significant importance and must be mitigated for safe and reproducible cell direct writing. The objective of this study is to investigate the cell membrane stability under the external normal pressure. This investigation is performed by studying the dipalmitoylphosphatidylcholine bilayer behavior under different normal pressures using molecular dynamics. As the normal pressure increases, the force necessary to rupture the bilayer structure decreases, which indicates cell membrane instability under high normal pressure. This phenomenon can also be explained by the change of free energy difference before and after rupture under different normal pressures. The effect of the pulling speed on the rupture force is also investigated, showing that the rupture force increases almost linearly with the pulling speed.


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