First report on genome size and ploidy determination of five indigenous coffee species using flow cytometry and stomatal analysis

2021 ◽  
Author(s):  
Pavankumar Jingade ◽  
Arun Kumar C. Huded ◽  
Manoj Kumar Mishra
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
First Report ◽  
Ploidy Determination ◽  
Coffee Species

scholarly journals GENOME SIZE DETERMINATION OF CUCUMBER (CUCUMIS SATIVUS), HONEYDEW (CUCUMIS MELO INODORUS) AND ROCK MELON (CUCUMIS MELO CANTALUPENSIS) VIA FLOW CYTOMETRY

2021 ◽  
Vol 5 (1) ◽  
pp. 14-16
Author(s):  
Raden Muhamad Imaduddin Yumni ◽  
Mohd Fauzihan Karim ◽  
Mohd Razik Midin
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Cucumis Melo ◽  
As Species ◽  
External Reference ◽  
The Family ◽  
Cucumis Species ◽  
Fcm Analysis ◽  
Interspecific And Intraspecific Variation

The family of Cucurbitaceae consists of species with economical and nutritional value. Morphologically, there are only few differences between Cucumis species. The interspecific and intraspecific variation in the genome size of the Cucumis species are not discovered yet. Due to this, this study aims to determine the genome size of C. sativus, C. melo inodorus and C. melo cantalupensis using flow cytometry (FCM) method. Nuclei suspension of selected Cucumis species were extracted using LBO1 lysis buffer by manual chopping technique and stained by propidium iodide priot to FCM analysis. Genome size of C. sativus, C. melo inodorus (Honeydew) and C. melo cantalupensis (Rockmelon) were determined by using Glycine max (Soybean) as an external reference standard (2C = 2.5 pg). This study found that the genome size of C. sativus, C. melo inodorus and C. melo cantalupensis estimated to be 2.83 pg, 3.00 pg and 3.47 pg respectively. The genome size data obtained from this study can be used in future genome studies as well as species characterization.

Ploidy Determination of Parthenogenetic Artemia by Flow Cytometry

2011 ◽  
Vol 474-476 ◽  
pp. 605-608
Author(s):  
Su Feng Wang ◽  
Yan Wang
Keyword(s):  
Flow Cytometry ◽  
New Method ◽  
Basic Data ◽  
Biological Study ◽  
Ploidy Determination

The ploidy of parthenogenetic Artemia from Aqqikkol Hu (Xinjiang) was determinated by flow cytometry. The results showed that the Artemia populaton of Aqqikkol Hu (Xinjiang) consisted diploid and tetraploid, the rate of diploid and tetraploid approximated to 80% and 20% respectively. It should conclude that the results of ploidy of parthenogenetic Artemia can be determinated rapidly and exactly by flow cytometry. The new method for the ploidy determination of parthenogenetic Artemia should accumulate the basic data for biological study and application of Artemia.

scholarly journals Nuclear Genome Size Determination Of Christia Vespertilionis Via Flow Cytometry

2020 ◽  
Vol 4 (2) ◽  
pp. 72-75
Author(s):  
Mohd Razik Midin ◽  
Muhammad Irfan Fikri ◽  
Siti Sarah Zailani
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Nuclear Genome ◽  
Size Determination ◽  
Size Estimation ◽  
Butterfly Wing ◽  
External Reference ◽  
Fcm Analysis ◽  
Suspension Preparation

AbstractChristia vespertilionis (butterfly wing plant) is an ornamental plant originated from South East Asia with reported usage in traditional medicine practice and potential as an anticancer and antitumor. This research aims to estimate the genome size of C. vespertilionis via flow cytometry (FCM) method. The research was conducted with the optimisation of nuclear suspension preparation followed by the genome size estimation. Two chopping techniques [manual chopping (MC) and BDTM Medimachine (MM)] and two lysis buffers (Otto and LBO1) were tested. Otto buffer with manual chopping was found to be the most suitable method, generated fine DNA peak with minimum debris background, and coefficient of variation (CV) value less than 3%. Five replicates of the FCM analysis were made for the genome size determination. The estimated genome size of C. vespertilionis was found to be 3.22 pg by using Glycine max cv. Polanka (2C=2.5pg) as an external reference standard. Further comparison with other Christia species was not possible due to the lack of data on genome size. The genome size data of C. vespertilionis can be useful for future morphology and genetics studies of Christia species.

scholarly journals Determination of genome size variations among different date palm cultivars (Phoenix dactylifera L.) by flow cytometry

3 Biotech ◽  
2019 ◽  
Vol 9 (12) ◽  
Author(s):  
Tahira Jatt ◽  
Moon-Sub Lee ◽  
A. Lane Rayburn ◽  
Mushtaque Ahmed Jatoi ◽  
Abdul Aziz Mirani
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Phoenix Dactylifera L

scholarly journals Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses

Caryologia ◽  
2017 ◽  
Vol 71 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Mohd Razik Midin ◽  
Mohd Shukor Nordin ◽  
Maria Madon ◽  
Mohd Nazre Saleh ◽  
Hoe-Han Goh ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Chromosome Number ◽  
Genome Size ◽  
Garcinia Mangostana

Determination of genome size and chromosome ploidy of selected taxa from Prunus armeniaca by flow cytometry

2020 ◽  
Vol 261 ◽  
pp. 108987 ◽  
Author(s):  
Wenwen Li ◽  
Liqiang Liu ◽  
Yanan Wang ◽  
Guoquan Fan ◽  
Shikui Zhang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Prunus Armeniaca

Ploidy Determination of Alfalfa Germplasm Accessions Using Flow Cytometry

Crop Science ◽  
1999 ◽  
Vol 39 (4) ◽  
pp. 1202-1207 ◽  
Author(s):  
E. Charles Brummer ◽  
Patricia M. Cazcarro ◽  
Diane Luth
Keyword(s):  
Flow Cytometry ◽  
Ploidy Determination ◽  
Germplasm Accessions

scholarly journals Cytogenetic relationships within the Maghrebian clade of Festuca subgen. Schedonorus (Poaceae), using flow cytometry and FISH

2017 ◽  
Vol 74 (1) ◽  
pp. 052 ◽  
Author(s):  
David Ezquerro-López ◽  
David Kopecký ◽  
Luis Á. Inda
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Fluorescent In Situ Hybridization ◽  
Genome Duplication ◽  
Size Estimation ◽  
Ploidy Levels ◽  
New Information ◽  
Shed Light

Festuca subgen. Schedonorus is a group of broad-leaved fescues, which can be divided into two clades: European and Maghrebian. We employed fluorescent in situ hybridization —FISH— with probes specific for 5S and 35S ribosomal DNA and genome size estimation using flow cytometry to shed light on the determination of possible parental genomes of polyploid species of the Maghrebian clade. Our results indicate that octoploid F. arundinacea subsp. atlantigena probably originated from crossing of the tetraploids F. arundinacea subsp. fenas —2n = 4x = 28— and F. mairei —2n = 4x = 28— followed by whole genome duplication. However, a large reconstruction of karyotype and genome downsizing has been revealed. Similarly, hexaploid F. arundinacea subsp. corsica presumably resulted from the interspecific hybridization of the diploid F. pratensis and tetraploid F. arundinacea subsp. fenas. Several scenarios on the origin of decaploid F. arundinacea var. letourneuxiana are discussed. This study contributed to our knowledge on the phylogeny of broad-leaved fescues and provided new information on the karyotypes —chromosome numbers, ploidy levels and numbers and positions of rDNA loci— using FISH and genome size estimations using flow cytometry in selected taxa of this important grass genus.

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