scholarly journals Biomass Microcapsules with Stem Cell Encapsulation for Bone Repair

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lei Yang ◽  
Yuxiao Liu ◽  
Lingyu Sun ◽  
Cheng Zhao ◽  
Guopu Chen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bone Repair ◽  
Biological Activities ◽  
Direct Injection ◽  
Animal Experiments ◽  
Cell Encapsulation ◽  
Bone Defects ◽  
Core Shell ◽  
Therapy Effect ◽  
Good Biocompatibility

Abstract Bone defects caused by trauma, tumor, or osteoarthritis remain challenging due to the lack of effective treatments in clinic. Stem cell transplantation has emerged as an alternative approach for bone repair and attracted widespread attention owing to its excellent biological activities and therapy effect. The attempts to develop this therapeutic approach focus on the generation of effective cell delivery vehicles, since the shortcomings of direct injection of stem cells into target tissues. Here, we developed a novel core-shell microcapsule with a stem cell-laden core and a biomass shell by using all-aqueous phase microfluidic electrospray technology. The designed core-shell microcapsules showed a high cell viability during the culture procedure. In addition, the animal experiments exhibited that stem cell-laden core-shell microcapsules have good biocompatibility and therapeutic effect for bone defects. This study indicated that the core-shell biomass microcapsules generated by microfluidic electrospray have promising potential in tissue engineering and regenerative medicine.

Regeneration of Bone Defects Using Bioactive Glass Combined with Adipose-derived Mesenchymal Stem Cells. An experimental in vivo study

2019 ◽  
Vol 70 (6) ◽  
pp. 1983-1987
Author(s):  
Cristian Trambitas ◽  
Anca Maria Pop ◽  
Alina Dia Trambitas Miron ◽  
Dorin Constantin Dorobantu ◽  
Flaviu Tabaran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bioactive Glass ◽  
Bone Repair ◽  
Bone Defects ◽  
Calvarial Bone ◽  
Specific Protocol ◽  
Repair Mechanisms ◽  
Male Wistar Rats

Large bone defects are a medical concern as these are often unable to heal spontaneously, based on the host bone repair mechanisms. In their treatment, bone tissue engineering techniques represent a promising approach by providing a guide for osseous regeneration. As bioactive glasses proved to have osteoconductive and osteoinductive properties, the aim of our study was to evaluate by histologic examination, the differences in the healing of critical-sized calvarial bone defects filled with bioactive glass combined with adipose-derived mesenchymal stem cells, compared to negative controls. We used 16 male Wistar rats subjected to a specific protocol based on which 2 calvarial bone defects were created in each animal, one was filled with Bon Alive S53P4 bioactive glass and adipose-derived stem cells and the other one was considered control. At intervals of one week during the following month, the animals were euthanized and the specimens from bone defects were histologically examined and compared. The results showed that this biomaterial was biocompatible and the first signs of osseous healing appeared in the third week. Bone Alive S53P4 bioactive glass could be an excellent bone substitute, reducing the need of bone grafts.

Application of Nanomaterials in Regulating the Fate of Adipose-derived Stem Cells

2021 ◽  
Vol 16 (1) ◽  
pp. 3-13
Author(s):  
Lang Wang ◽  
Yong Li ◽  
Maorui Zhang ◽  
Kui Huang ◽  
Shuanglin Peng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Stem Cell Research ◽  
Adult Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Proliferation And Differentiation ◽  
Recent Developments ◽  
Good Biocompatibility ◽  
Regulatory Effects ◽  
New Treatment

Adipose-derived stem cells are adult stem cells which are easy to obtain and multi-potent. Stem-cell therapy has become a promising new treatment for many diseases, and plays an increasingly important role in the field of tissue repair, regeneration and reconstruction. The physicochemical properties of the extracellular microenvironment contribute to the regulation of the fate of stem cells. Nanomaterials have stable particle size, large specific surface area and good biocompatibility, which has led them being recognized as having broad application prospects in the field of biomedicine. In this paper, we review recent developments of nanomaterials in adipose-derived stem cell research. Taken together, the current literature indicates that nanomaterials can regulate the proliferation and differentiation of adipose-derived stem cells. However, the properties and regulatory effects of nanomaterials can vary widely depending on their composition. This review aims to provide a comprehensive guide for future stem-cell research on the use of nanomaterials.

scholarly journals Biomaterial design strategies to address obstacles in craniomaxillofacial bone repair

RSC Advances ◽  
2021 ◽  
Vol 11 (29) ◽  
pp. 17809-17827
Author(s):  
Marley J. Dewey ◽  
Brendan A. C. Harley
Keyword(s):  
Bone Repair ◽  
Bone Defects ◽  
Design Strategies

There exist many challenges in the process of regenerating craniomaxillofacial bone defects, thus biomaterials must be designed to overcome these.

scholarly journals Biological Activities Encoded by the Murine Mesenchymal Stem Cell Transcriptome Provide a Basis for Their Developmental Potential and Broad Therapeutic Efficacy

Stem Cells ◽  
2006 ◽  
Vol 24 (1) ◽  
pp. 186-198 ◽  
Author(s):  
Donald G. Phinney ◽  
Katy Hill ◽  
Charles Michelson ◽  
Maria DuTreil ◽  
Catherine Hughes ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Therapeutic Efficacy ◽  
Biological Activities ◽  
Developmental Potential ◽  
Cell Transcriptome

scholarly journals Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor.

1994 ◽  
Vol 14 (2) ◽  
pp. 1431-1437 ◽  
Author(s):  
B Samal ◽  
Y Sun ◽  
G Stearns ◽  
C Xie ◽  
S Suggs ◽  
...  
Keyword(s):  
Stem Cell ◽  
B Cell ◽  
Stem Cell Factor ◽  
Human Peripheral Blood ◽  
Biological Activities ◽  
In Vitro Assays ◽  
Pokeweed Mitogen ◽  
Interleukin 7 ◽  
Enhancing Factor ◽  
Cell Colony

A novel gene coding for the pre-B-cell colony-enhancing factor (PBEF) has been isolated from a human peripheral blood lymphocyte cDNA library. The expression of this gene is induced by pokeweed mitogen and superinduced by cycloheximide. It is also induced in the T-lymphoblastoid cell line HUT 78 after phorbol ester (phorbol myristate acetate) treatment. The predominant mRNA for PBEF is approximately 2.4 kb long and codes for a 52-kDa secreted protein. The 3' untranslated region of the mRNA has multiple TATT motifs, usually found in cytokine and oncogene messages. The PBEF gene is mainly transcribed in human bone marrow, liver tissue, and muscle. We have expressed PBEF in COS 7 and PA317 cells and have tested the biological activities of the conditioned medium as well as the antibody-purified protein in different in vitro assays. PBEF itself had no activity but synergized the pre-B-cell colony formation activity of stem cell factor and interleukin 7. In the presence of PBEF, the number of pre-B-cell colonies was increased by at least 70% above the amount stimulated by stem cell factor plus interleukin 7. No effect of PBEF was found with cells of myeloid or erythroid lineages. These data define PBEF as a novel cytokine which acts on early B-lineage precursor cells.

Fabrication of pH Responsive Core-Shell Nanosystem with Low-Temperature Photothermal Therapy Effect for Treating Bacterial Biofilm Infection

2021 ◽  
Author(s):  
Dan Peng ◽  
Genhua Liu ◽  
Ye He ◽  
Pengfei Gao ◽  
Shuangquan Gou ◽  
...  
Keyword(s):  
Low Temperature ◽  
Photothermal Therapy ◽  
Normal Tissue ◽  
Bacterial Biofilm ◽  
Alternative Strategy ◽  
Viable Alternative ◽  
Core Shell ◽  
Ph Responsive ◽  
Therapy Effect ◽  
Biofilm Infection

Recently, photothermal therapy (PTT) has been recognized as a viable alternative strategy against bacterial biofilm infection. However, hyperthermia required for PTT to ablate biofilm usually induced damage of normal tissue/organ...

Polymeric scaffolds as stem cell carriers in bone repair

2013 ◽  
Vol 9 (10) ◽  
pp. 1093-1119 ◽  
Author(s):  
Filippo Rossi ◽  
Marco Santoro ◽  
Giuseppe Perale
Keyword(s):  
Stem Cell ◽  
Bone Repair ◽  
Polymeric Scaffolds ◽  
Cell Carriers
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