scholarly journals First report on Xanthomonas campestris pv. campestris causing bacterial black rot disease of cauliflower in Turkey

2018 ◽  
Vol 100 (1) ◽  
pp. 141-141
Author(s):  
Hasan Murat Aksoy ◽  
Murat Ozturk ◽  
Sedanur Tufan
Keyword(s):  
Xanthomonas Campestris ◽  
Black Rot ◽  
First Report ◽  
Rot Disease ◽  
Xanthomonas Campestris Pv Campestris

Involvement of a novel peroxidase isozyme and lignification in hydathodes in resistance to black rot disease in cabbage

2000 ◽  
Vol 78 (9) ◽  
pp. 1144-1149 ◽  
Author(s):  
P A Gay ◽  
S Tuzun
Keyword(s):  
Xanthomonas Campestris ◽  
Virulent Strain ◽  
Black Rot ◽  
Peroxidase Isozyme ◽  
Anionic Peroxidase ◽  
Peroxidase Isozymes ◽  
Resistant Varieties ◽  
Rot Disease ◽  
Xanthomonas Campestris Pv Campestris ◽  
Lignin Deposition

The physiological mechanisms associated with resistance of cabbage to black rot disease seem to be associated with the hydathodes. To investigate the role of hydathodes in disease resistance, total peroxidase activities, anionic peroxidase isozyme expression, and lignin deposition were determined in hydathodes of resistant (Hancock and Green Cup), partially resistant (Cheers), and susceptible (Strukton and Perfect Ball) cabbage varieties (Brassica oleracea L.) during pathogenesis with Xanthomonas campestris pv. campestris. Four-week-old plants were petiole-inoculated with a highly virulent strain of X. campestris pv. campestris (FD91L). Hydathodal fluids were collected daily over a 14-day period from infected plants as well as noninfected, mock-inoculated control plants. Hydathodal fluids of resistant varieties had greater peroxidase activity when compared to susceptible ones, with infected plants having higher peroxidase levels than noninfected plants. Isoelectric focusing revealed the presence of four anionic peroxidase isozymes in hydathodal fluids, with the most anionic one (pI of 3.6) accumulating only upon infection. Lignin deposition in and around the hydathodes was associated with the accumulation of this particular isozyme in hydathodal fluids. The evidence suggests that a rapid, systemic response is associated with resistance to the bacterial pathogen.Key words: peroxidases, hydathodes, isozymes, black rot disease, cabbage, Xanthomonas campestris pv. campestris.

scholarly journals Development of Efficient Screening Method for Resistance of Cabbage Cultivars to Black Rot Disease Caused by Xanthomonas campestris pv. campestris

2013 ◽  
Vol 19 (2) ◽  
pp. 95-101 ◽  
Author(s):  
Ji Hyun Lee ◽  
Jin-Cheol Kim ◽  
Kyoung Soo Jang ◽  
Yong Ho Choi ◽  
Kyoung Gu Ahn ◽  
...  
Keyword(s):  
Xanthomonas Campestris ◽  
Screening Method ◽  
Black Rot ◽  
Rot Disease ◽  
Efficient Screening ◽  
Xanthomonas Campestris Pv Campestris

scholarly journals Early Defense Mechanisms of Brassica oleracea in Response to Attack by Xanthomonas campestris pv. campestris

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2705
Author(s):  
Lu Lu ◽  
Sokrat G. Monakhos ◽  
Yong Pyo Lim ◽  
So Young Yi
Keyword(s):  
Brassica Oleracea ◽  
Defense Mechanisms ◽  
Xanthomonas Campestris ◽  
Radical Scavenging ◽  
Defense Responses ◽  
Black Rot ◽  
Enzymatic Antioxidants ◽  
Induced Expression ◽  
Rot Disease ◽  
Xanthomonas Campestris Pv Campestris

Black rot disease, caused by Xanthomonas campestris pv. campestris (Xcc), results in significant yield losses in Brassica oleracea crops worldwide. To find black rot disease-resistant cabbage lines, we carried out pathogenicity assays using the scissor-clipping method in 94 different B. oleracea lines. By comparing the lesion areas, we selected a relatively resistant line, Black rot Resistance 155 (BR155), and a highly susceptible line, SC31. We compared the two cabbage lines for the Xcc-induced expression pattern of 13 defense-related genes. Among them, the Xcc-induced expression level of PR1 and antioxidant-related genes (SOD, POD, APX, Trx H, and CHI) were more than two times higher in BR155 than SC31. Nitroblue tetrazolium (NBT) and diaminobenzidine tetrahydrochloride (DAB) staining analysis showed that BR155 accumulated less Xcc-induced reactive oxygen species (ROS) than did SC31. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays showed that BR155 had higher antioxidant activity than SC31. This study, focused on the defense responses of cabbage during the early biotrophic stage of infection, indicated that Xcc-induced ROS might play a role in black rot disease development. We suggest that non-enzymatic antioxidants are important, particularly in the early defense mechanisms of cabbage against Xcc.

scholarly journals A study on pathological aspects of Xanthomonas campestris pv. campestris causing black rot of cabbage under red lateritic zone of West Bengal

2015 ◽  
Vol 7 (2) ◽  
pp. 780-785
Author(s):  
Atit Maji ◽  
Ranjan Nath
Keyword(s):  
Xanthomonas Campestris ◽  
West Bengal ◽  
Maximum Growth ◽  
Black Rot ◽  
Total Production ◽  
Vegetable Crops ◽  
Management Options ◽  
Rot Disease ◽  
Nutritional Studies ◽  
Xanthomonas Campestris Pv Campestris

Cabbage, is one of the most important crops of the cole group of vegetables. In India it ranks next to cauliflower in acreage and first in production among cole crops occupying an area of 3,72,000 ha with annual production of 8534,000 tons. It covers about 4.3% area under vegetable crops in India. In West Bengal cabbage covers 78200.00 ha of area and the total production is 2197400.00 MT. Black rot is a major disease of cabbage (Brassica oleracea var. capitata), caused by Xanthomonas campestris pv. campestris (Xcc) . The disease has been observed in all cabbage growing areas of Bolpur, Birbhum, West Bengal. The present study was carried out on the pathology of black rot disease of cabbage. Morphological, cultural, biochemical, and physiological characteristics of the pathogen were studied. The bacterium produced small, yellow, circular, entire, smooth and shining colonies in the culture medium. The optimum temperature for the growth was found 300C and white light supported maximum growth of the bacterium. Nutritional studies revealed that sucrose gave maximum growth followed by maltase, lactose, dextrose and fructose as the carbon source in the nutrient broth. Black rot of cabbage pathogen also infected other crops of crucifereae family such as Cauliflower, Knol khol, Mustard, Radish and Rape seed. These findings regarding the pathogen may help to formulate the more appropriate way and judicious application of different management options against the diease in this zone.

scholarly journals First Report of Black Rot on Arugula Caused by Xanthomonas campestris pv. campestris in Argentina

Plant Disease ◽  
2008 ◽  
Vol 92 (6) ◽  
pp. 980-980 ◽  
Author(s):  
A. M. Romero ◽  
R. Zapata ◽  
M. S. Montecchia
Keyword(s):  
Xanthomonas Campestris ◽  
Pathogenic Bacteria ◽  
Biochemical Characterization ◽  
Bacterial Suspension ◽  
Black Rot ◽  
Strictly Aerobic ◽  
First Report ◽  
Plastic Bags ◽  
Water Plants ◽  
Xanthomonas Campestris Pv Campestris

During the fall of 2005, arugula (Eruca sativa Mill.) plants grown in experimental field plots in Buenos Aires, Argentina presented V-shaped necrotic lesions on leaf margins and blackened veins with broad yellow halos, followed by leaf necrosis. At flowering, 96% of the plants were affected with 27% of the leaves with symptoms. Yellow, round, mucoid, convex, bacterial colonies were isolated from several leaves on yeast dextrose chalk agar. Two strains were further studied. Xanthomonas campestris pv. campestris Xcc8004 was used as a control. Strains were gram negative, rod shaped, strictly aerobic, catalase-positive, oxidase and urease-negative, hydrolyzed starch, gelatine and aesculin, and did not reduce nitrate (2). Pathogenicity was tested by spraying 10 3-week-old arugula plants with either a bacterial suspension (107 CFU/ml) or sterile water. Plants were placed in plastic bags for 72 h after inoculation. All inoculated plants showed necrotic lesions enlarging from the margin of the leaves 7 days after inoculation. No lesions were observed on control plants. On the basis of biochemical characterization (2) and genomic fingerprints generated by BOX-PCR (1), the pathogen was identified as X. campestris pv. campestris. To our knowledge, this is the first report of X. campestris pv. campestris causing black rot on arugula in Argentina. References: (1) J. L. Rademaker et al. Int. J. Syst. Evol. Microbiol. 50:665, 2000. (2) N. W. Schaad et al. Laboratory Guide for Identification of Plant Pathogenic Bacteria. 3rd ed. The American Phytopathological Society, St. Paul, MN, 2001.

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