Beneficial effects of Rhizophagus irregularis and Trichoderma asperellum strain T34 on growth and fusarium wilt in tomato plants

Abstract Composts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial effects towards plants. Here, we characterized a collection of compost-derived bacteria, previously isolated from tomato rhizosphere, for in vitro antifungal activity against soil-borne fungal pathogens and for their potential to change growth parameters in Arabidopsis. We further assessed root-competitive traits in the dominant rhizospheric genus Bacillus. Certain isolated rhizobacteria displayed antifungal activity against the tested pathogens and affected growth of Arabidopsis, and Bacilli members possessed several enzymatic activities. Subsequently, we designed two SynComs with different composition and tested their effect on Arabidopsis and tomato growth and health. SynCom1, consisting of different bacterial genera, displayed negative effect on Arabidopsis in vitro, but promoted tomato growth in pots. SynCom2, consisting of Bacilli, didn't affect Arabidopsis growth, enhanced tomato growth and suppressed Fusarium wilt symptoms. Overall, we found selection of compost-derived microbes with beneficial properties in the rhizosphere of tomato plants, and observed that application of SynComs on poor substrates can yield reproducible plant phenotypes.

Download Full-text

Rhizosphere-enriched microbes as a pool to design synthetic communities for reproducible beneficial outputs

10.1101/488064 ◽

2018 ◽

Cited By ~ 1

Author(s):

Maria-Dimitra Tsolakidou ◽

Ioannis A. Stringlis ◽

Natalia Fanega-Sleziak ◽

Stella Papageorgiou ◽

Antria Tsalakou ◽

...

Keyword(s):

Antifungal Activity ◽

Fungal Pathogens ◽

Growth Parameters ◽

Tomato Plants ◽

Beneficial Effects ◽

Negative Effect ◽

Tomato Growth ◽

Selection Of

AbstractComposts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial effects towards plants. Here, we characterized a collection of compost-derived bacteria, previously isolated from tomato rhizosphere, forin vitroantifungal activity against soil-borne fungal pathogens and for their potential to change growth parameters inArabidopsis. We further assessed root-competitive traits in the dominant rhizospheric genusBacillus. Certain isolated rhizobacteria displayed antifungal activity against the tested pathogens and affected growth ofArabidopsis, and Bacilli members possessed several enzymatic activities. Subsequently, we designed two SynComs with different composition and tested their effect onArabidopsisand tomato growth and health. SynCom1, consisting of different bacterial genera, displayed negative effect onArabidopsis in vitro, but promoted tomato growth in pots. SynCom2, consisting of Bacilli, didn’t affectArabidopsisgrowth, enhanced tomato growth and suppressed Fusarium wilt symptoms. Overall, we found selection of compost-derived microbes with beneficial properties in the rhizosphere of tomato plants, and observed that application of SynComs on poor substrates can yield reproducible plant phenotypes.

Download Full-text

Resistance in Tomato Plants Against Fusarium Wilt Induced by Fusarium Oxysporum F. Sp. Dianthi.

Biotic Interactions and Soil-Borne Diseases - Developments in Agricultural and Managed Forest Ecology ◽

10.1016/b978-0-444-88728-3.50058-5 ◽

1991 ◽

pp. 306-310 ◽

Cited By ~ 1

Author(s):

B.A.M. KROON ◽

R.J. SCHEFFER ◽

D.M. ELGERSMA

Keyword(s):

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Tomato Plants

Download Full-text

Synergetic Effect of Rhizosphere Bacteria Isolates and Composted Manure on Fusarium Wilt Disease of Tomato Plants

Research Journal of Microbiology ◽

10.3923/jm.2016.20.27 ◽

2016 ◽

Vol 11 (1) ◽

pp. 20-27 ◽

Cited By ~ 1

Author(s):

Anbessa Dabassa ◽

Lata Lachisa

Keyword(s):

Fusarium Wilt ◽

Synergetic Effect ◽

Wilt Disease ◽

Rhizosphere Bacteria ◽

Tomato Plants ◽

Fusarium Wilt Disease

Download Full-text

Application of Trichoderma harzianum, 6-Pentyl-α-pyrone and Plant Biopolymer Formulations Modulate Plant Metabolism and Fruit Quality of Plum Tomatoes

Plants ◽

10.3390/plants9060771 ◽

2020 ◽

Vol 9 (6) ◽

pp. 771 ◽

Cited By ~ 4

Author(s):

Petronia Carillo ◽

Sheridan L. Woo ◽

Ernesto Comite ◽

Christophe El-Nakhel ◽

Youssef Rouphael ◽

...

Keyword(s):

Trichoderma Harzianum ◽

Total Yield ◽

Plant Metabolism ◽

Tomato Plants ◽

Beneficial Effects ◽

Greenhouse Tomato ◽

Solanum Lycopersicum L ◽

Functional Quality ◽

Stimulate Plant Growth

Many Trichoderma are successfully used to improve agriculture productivity due to their capacity for biocontrol and to stimulate plant growth and tolerance to abiotic stress. This research elucidates the effect of applications with Trichoderma harzianum strain T22 (T22), or biopolymer (BP) alone or in combination (BP + T22 or BP + 6-pentyl-α-pyrone (6PP); a Trichoderma secondary metabolite) on the crop performance, nutritional and functional quality of greenhouse tomato (Solanum lycopersicum L. cultivar Pixel). T22 elicited significant increases in total yield (+40.1%) compared to untreated tomato. The content of lycopene, an important antioxidant compound in tomatoes, significantly increased upon treatment with T22 (+ 49%), BP + T22 (+ 40%) and BP + 6PP (+ 52%) compared to the control. T22 treatments significantly increased the content of asparagine (+37%), GABA (+87%) and MEA (+102%) over the control; whereas BP alone strongly increased GABA (+105%) and MEA (+85%). The synthesis of these compounds implies that tomato plants are able to reuse the photorespiratory amino acids and ammonium for producing useful metabolites and reduce the pressure of photorespiration on plant metabolism, thus optimizing photosynthesis and growth. Finally, these metabolites exert many beneficial effects for human health, thus enhancing the premium quality of plum tomatoes.

Download Full-text

Presymptomatic detection of Fusarium wilt of tomato by electrical resistance measurement as related to pectic enzyme production

Canadian Journal of Botany ◽

10.1139/b76-081 ◽

1976 ◽

Vol 54 (8) ◽

pp. 752-757 ◽

Cited By ~ 3

Author(s):

Frank L. Caruso ◽

Terry A. Tattar ◽

Mark S. Mount ◽

Margaret E. Malia

Keyword(s):

Fusarium Wilt ◽

Electrical Resistance ◽

Wilt Disease ◽

Symptom Development ◽

In Vitro Production ◽

Tomato Plants ◽

Foliar Symptom ◽

Vitro Production ◽

Soil Level

Decreases in electrical resistance (ER) of tomato taproots were correlated with the progression of Fusarium wilt disease. Production of polygalacturonase (PG) from four isolates of Fusarium oxysporum f. sp. lycopersici, as determined from ammonium sulfate fractions, was correlated with the degree of virulence of each isolate. A drop in ER in tomato plant taproots at 6 cm below the soil level was noticed within 2 h after separate inoculation with all strains. These drops in ER preceded foliar symptom development by 20 h. Measurement of ER in the stems of inoculated tomato plants, however, was found to be no different from that of uninoculated plants. The rate of ER decrease was correlated with the in vitro production of PG by these isolates; the higher the PG activity, the greater the decrease in ER.

Download Full-text