First report of bean leafroll virus (BLRV) naturally infecting common and bitter vetch and alfalfa in Greece

Although all three viruses are commonly found in potatoes throughout the world, this is the first report of potato viruses from Alaska to be sequenced and molecularly analyzed for comparisons with known viruses. Accepted for publication 17 January 2011. Published 9 February 2011.

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Screening and selection of faba bean (Vicia faba L.) germplasm resistant to Bean leafroll virus

Australian Journal of Agricultural Research ◽

10.1071/ar01202 ◽

2002 ◽

Vol 53 (9) ◽

pp. 1077 ◽

Cited By ~ 13

Author(s):

Khaled M. Makkouk ◽

Safaa G. Kumari ◽

Joop A. G. van Leur

Keyword(s):

Faba Bean ◽

Virus Concentration ◽

Visual Evaluation ◽

Large Variability ◽

Vicia Faba L ◽

Bean Leafroll Virus ◽

Tissue Blot Immunoassay ◽

Selection For ◽

Leafroll Virus ◽

Selection Of

Three hundred and fifty-eight faba bean accessions, originating from 28 countries in 5 continents, were evaluated for their reaction to a Syrian isolate (SV64-95) of Bean leafroll virus (BLRV, family Luteoviridae). Selection for resistance was based on: (i) visual evaluation for symptom expression (disease severity); (ii) virus concentration in the plant tissues, based on the intensity of reaction in the tissue-blot immunoassay; and (iii) seed yield. A large variability in resistance to BLRV was found both between and within faba bean accessions. Progenies of single plants, selected for resistance under inbreeding conditions, were evaluated for another 2-year period. Repeated reselection of inoculated plants, in the absence of outcrossing, significantly increased the proportion of resistant plants. The most resistant selections were made from populations originating from Afghanistan, China, Colombia, Pakistan, Russia, Sudan, Switzerland, Tunisia, Turkey, and Yemen.

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Bean leafroll virus is widespread in subterranean clover (Trifolium subterraneum L.) seed crops and can be persistently transmitted by bluegreen aphid (Acyrthosiphon kondoi Shinji)

Crop and Pasture Science ◽

10.1071/cp12121 ◽

2012 ◽

Vol 63 (9) ◽

pp. 902 ◽

Cited By ~ 5

Author(s):

D. M. Peck ◽

N. Habili ◽

R. M. Nair ◽

J. W. Randles ◽

C. T. de Koning ◽

...

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Seed Production ◽

Alfalfa Mosaic Virus ◽

South Australia ◽

Subterranean Clover ◽

Trifolium Subterraneum ◽

Bean Leafroll Virus ◽

Leafroll Virus ◽

Clover Seed

In the mid 2000s subterranean clover (Trifolium subterraneum) seed producers in South Australia reported symptoms of a red-leaf disease in fields with reduced seed yields. The red-leaf symptoms resembled those caused by several clover-infecting viruses. A set of molecular diagnostic tools were developed for the following viruses which are known to infect subterranean clover: Alfalfa mosaic virus; Bean leafroll virus (BLRV); Beet western yellows virus; Bean yellow mosaic virus; Cucumber mosaic virus; Pea seed-borne mosaic virus; Soybean dwarf virus and Subterranean clover stunt virus. Surveys of subterranean clover seed production fields in 2008 in the south-east of South Australia and western Victoria identified Bean leafroll virus, Alfalfa mosaic virus and Cucumber mosaic virus as present, with BLRV the most widespread. Surveys of pasture seed production fields and pasture evaluation trials in 2009 confirmed that BLRV was widespread. This result will allow seed producers to determine whether control measures directed against BLRV will overcome their seed losses. Bluegreen aphid (Acyrthosiphon kondoi) was implicated as a potential vector of BLRV because it was observed to be colonising lucerne plants adjacent to subterranean clover seed production paddocks with BLRV, and in a glasshouse trial it transmitted BLRV from an infected lucerne plant to subterranean clover in a persistent manner.

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Differentiation Among Bean Leafroll Virus Susceptible and Resistant Lentil and Faba Bean Genotypes on the Basis of Virus Movement and Multiplication

Journal of Phytopathology ◽

10.1046/j.1439-0434.2003.00673.x ◽

2003 ◽

Vol 151 (1) ◽

pp. 19-25 ◽

Cited By ~ 7

Author(s):

S. G. Kumari ◽

K. M. Makkouk

Keyword(s):

Faba Bean ◽

Virus Movement ◽

Bean Leafroll Virus ◽

Leafroll Virus

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First report ofPotato leafroll virus,Potato virus A,Potato virus XandPotato virus Yin potato in Greenland

New Disease Reports ◽

10.5197/j.2044-0588.2014.030.020 ◽

2014 ◽

Vol 30 ◽

pp. 20 ◽

Cited By ~ 2

Author(s):

E. de Neergaard ◽

L. Munk ◽

S.L. Nielsen

Keyword(s):

Potato Virus ◽

First Report ◽

Potato Virus A ◽

Leafroll Virus

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First Report of Cherry necrotic rusty mottle virus Infecting Sweet Cherry Trees in Korea

Plant Disease ◽

10.1094/pdis-07-13-0723-pdn ◽

2014 ◽

Vol 98 (1) ◽

pp. 164-164 ◽

Cited By ~ 3

Author(s):

I. S. Cho ◽

G. S. Choi ◽

S. K. Choi ◽

E. Y. Seo ◽

H. S. Lim

Keyword(s):

Sweet Cherry ◽

Mottle Virus ◽

Post Inoculation ◽

Rt Pcr ◽

First Report ◽

Coat Protein Region ◽

Pcr Products ◽

Leafroll Virus ◽

Leaf Virus ◽

Cherry Trees

Cherry necrotic rusty mottle virus (CNRMV), an unassigned member in the family Betaflexiviridae, has been reported in sweet cherry in North America, Europe, New Zealand, Japan, China, and Chile. The virus causes brown, angular necrotic spots, shot holes on the leaves, gum blisters, and necrosis of the bark in several cultivars (1). During the 2012 growing season, 154 sweet cherry trees were tested for the presence of CNRMV by RT-PCR. Samples were randomly collected from 11 orchards located in Gyeonggi and Gyeongsang provinces in Korea. RNA was extracted from leaves using the NucliSENS easyMAG system (bioMérieux, Boxtel, The Netherlands). The primer pair CGRMV1/2 (2) was used to amplify the coat protein region of CNRMV. Although none of the collected samples showed any notable symptoms, CNRMV PCR products of the expected size (949 bp) were obtained from three sweet cherry samples from one orchard in Gyeonggi province. The PCR products were cloned into a pGEM-T easy vector (Promega, Madison, WI) and sequenced. BLAST analyses of the three Korean sequences obtained (GenBank Accession Nos. AB822635, AB822636, and AB822637) showed 97% nucleotide sequence identity with a flowering cherry isolate from Japan (EU188439), and shared 98.8 to 99.6% nucleotide and 99.6 to 100% amino acid similarities to each other. The CNRMV positive samples were also tested for Apple chlorotic leaf spot virus (ACLSV), Cherry mottle leaf virus (CMLV), Cherry rasp leaf virus (CRLV), Cherry leafroll virus (CLRV), Cherry virus A (CVA), Little cherry virus 1 (LChV-1), Prune dwarf virus (PDV), and Prunus necrotic ringspot virus (PNRSV) by RT-PCR. One of the three CNRMV-positive samples was also infected with CVA. To confirm CNRMV infection by wood indexing, Prunus serrulata cv. Kwanzan plants were graft-inoculated with chip buds from the CNRMV-positive sweet cherry trees. At 3 to 4 weeks post-inoculation, the Kwanzan plants showed quick decline with leaves wilting and dying; CNRMV infection of the indicators was confirmed by RT-PCR. To our knowledge, this is the first report of CNRMV infection of sweet cherry trees in Korea. Screening for CNRMV in propagation nurseries should minimize spread of this virus within Korea. References: (1) R. Li and R. Mock. Arch. Virol. 153:973, 2008. (2) R. Li and R. Mock. J. Virol. Methods 129:162, 2005.

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First Report of Solanum sarrachoides (Hairy Nightshade) as an Important Host of Potato leafroll virus

Plant Disease ◽

10.1094/pdis.2002.86.5.559c ◽

2002 ◽

Vol 86 (5) ◽

pp. 559-559 ◽

Cited By ~ 11

Author(s):

P. E. Thomas

Keyword(s):

Green Peach Aphid ◽

Potato Crop ◽

Potato Leafroll Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Columbia Basin ◽

First Report ◽

Narrow Host Range ◽

Potato Plants ◽

Solanum Sarrachoides ◽

Leafroll Virus

Potato leafroll virus (PLRV) (genus Polerovirus, family Luteoviridae) is of great economic importance. It has a very narrow host range consisting of only 21 known species, 14 of which are Solanaceae. Ten aphid species transmit PLRV in a persistent manner, but the green peach aphid (Myzus persicae Sulzer) is by far the most important vector and the only important vector in the Columbia Basin of the Pacific Northwest (3). Solanum sarrachoides (Sendtner) may be especially important in the epidemiology of PLRV because it is a predominant weed in potatoes (S. tuberosum L.), other annual crops, and waste areas of the Columbia Basin (1), and herbicides available for potato are relatively ineffective against this weed (2). Since our research began in 1974, we have routinely observed many S. sarrachoides plants throughout the Columbia Basin that acquired an abnormal erect stiff appearance in middle to late summer. Their leaflets curled upward at the margins and expressed interveinal chlorosis and necrosis and a reddened or purple color on abaxial surfaces. The presence of PLRV in symptomatic plants was routinely detected by double-antibody sandwich enzyme-linked immunosorbent assay and by density gradient sedimentation and light absorbance properties of the purified virus when we evaluated the host as a source of purified PLRV in 1980. A systematic study to satisfy the requirements of Koch's postulates to prove that PLRV causes the disease in S. sarrachoides was conducted in 1998. The virus produced typical PLRV symptoms on three young Physalis floridana, Datura stramonium, and potato plants when transmitted by the green peach aphid from each of four symptomatic S. sarrachoides collected from widely spaced potato fields in the Columbia Basin and from purified virus preparations from these plants. Back-transmission from each of these hosts and from purified preparations to S. sarrachoides plants observed the same symptoms. The green peach aphid readily colonized S. sarrachoides in the field, and populations on this host were typically higher than on adjacent potato plants in potato fields. The concentration of PLRV was approximately equal in infected S. sarrachoides and P. floridana plants, as was the efficiency of virus transmission from these hosts by individual green peach aphids. The aphids used in transmission efficacy assays were all the same clone and were reared on the host species assayed. The incidence of PLRV infection among S. sarrachoides in potato fields was typically higher than in the potato crop. Only two other summer annual hosts of PLRV, Amaranthus caudatus and S. nigrum, occur sporadically in potato fields of the Columbia Basin (1). The virus rarely infected these species. To our knowledge, this is the first report that S. sarrachoides is highly susceptible to PLRV and may play an important role in PLRV epidemiology in the Columbia Basin. References: (1) A. G. Ogg, Jr. and B. S. Rogers. Rev. Weed Sci. 4:25, 1989. (2) L. S. Quakenbush and R. N. Anderson. Weed Sci. 33:386, 1985. (3) P. E. Thomas et al. Plant Dis. 81:1311, 1997.

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