Differentiation Among Bean Leafroll Virus Susceptible and Resistant Lentil and Faba Bean Genotypes on the Basis of Virus Movement and Multiplication

Three hundred and fifty-eight faba bean accessions, originating from 28 countries in 5 continents, were evaluated for their reaction to a Syrian isolate (SV64-95) of Bean leafroll virus (BLRV, family Luteoviridae). Selection for resistance was based on: (i) visual evaluation for symptom expression (disease severity); (ii) virus concentration in the plant tissues, based on the intensity of reaction in the tissue-blot immunoassay; and (iii) seed yield. A large variability in resistance to BLRV was found both between and within faba bean accessions. Progenies of single plants, selected for resistance under inbreeding conditions, were evaluated for another 2-year period. Repeated reselection of inoculated plants, in the absence of outcrossing, significantly increased the proportion of resistant plants. The most resistant selections were made from populations originating from Afghanistan, China, Colombia, Pakistan, Russia, Sudan, Switzerland, Tunisia, Turkey, and Yemen.

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Host-Dependent Requirement for the Potato leafroll virus 17-kDa Protein in Virus Movement

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.2002.15.10.1086 ◽

2002 ◽

Vol 15 (10) ◽

pp. 1086-1094 ◽

Cited By ~ 47

Author(s):

Lawrence Lee ◽

Peter Palukaitis ◽

Stewart M. Gray

Keyword(s):

Amino Acids ◽

Solanum Tuberosum ◽

Nicotiana Benthamiana ◽

Systemic Infection ◽

Potato Leafroll Virus ◽

Virus Movement ◽

Wild Type ◽

Mature Leaves ◽

Leafroll Virus ◽

Virus Distribution

The requirement for the 17-kDa protein (P17) of Potato leafroll virus (PLRV) in virus movement was investigated in four plant species: potato (Solanum tuberosum), Physalis floridana, Nicotiana benthamiana, and N. clevelandii. Two PLRV P17 mutants were characterized, one that does not translate the P17 and another that expresses a P17 missing the first four amino acids. The P17 mutants were able to replicate and accumulate in agroinoculated leaves of potato and P. floridana, but they were unable to move into vascular tissues and initiate a systemic infection in these plants. In contrast, the P17 mutants were able to spread systemically from inoculated leaves in both Nicotiana spp., although the efficiency of infection was reduced relative to wild-type PLRV. Examination of virus distribution in N. benthamiana plants using tissue immunoblotting techniques revealed that the wild-type PLRV and P17 mutants followed a similar movement pathway out of the inoculated leaves. Virus first moved upward to the apical tissues and then downward. The P17 mutants, however, infected fewer phloem-associated cells, were slower than wild-type PLRV in moving out of the inoculated tissue and into apical tissues, and were unable to infect any mature leaves present on the plant at the time of inoculation.

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Bean leafroll virus is widespread in subterranean clover (Trifolium subterraneum L.) seed crops and can be persistently transmitted by bluegreen aphid (Acyrthosiphon kondoi Shinji)

Crop and Pasture Science ◽

10.1071/cp12121 ◽

2012 ◽

Vol 63 (9) ◽

pp. 902 ◽

Cited By ~ 5

Author(s):

D. M. Peck ◽

N. Habili ◽

R. M. Nair ◽

J. W. Randles ◽

C. T. de Koning ◽

...

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Seed Production ◽

Alfalfa Mosaic Virus ◽

South Australia ◽

Subterranean Clover ◽

Trifolium Subterraneum ◽

Bean Leafroll Virus ◽

Leafroll Virus ◽

Clover Seed

In the mid 2000s subterranean clover (Trifolium subterraneum) seed producers in South Australia reported symptoms of a red-leaf disease in fields with reduced seed yields. The red-leaf symptoms resembled those caused by several clover-infecting viruses. A set of molecular diagnostic tools were developed for the following viruses which are known to infect subterranean clover: Alfalfa mosaic virus; Bean leafroll virus (BLRV); Beet western yellows virus; Bean yellow mosaic virus; Cucumber mosaic virus; Pea seed-borne mosaic virus; Soybean dwarf virus and Subterranean clover stunt virus. Surveys of subterranean clover seed production fields in 2008 in the south-east of South Australia and western Victoria identified Bean leafroll virus, Alfalfa mosaic virus and Cucumber mosaic virus as present, with BLRV the most widespread. Surveys of pasture seed production fields and pasture evaluation trials in 2009 confirmed that BLRV was widespread. This result will allow seed producers to determine whether control measures directed against BLRV will overcome their seed losses. Bluegreen aphid (Acyrthosiphon kondoi) was implicated as a potential vector of BLRV because it was observed to be colonising lucerne plants adjacent to subterranean clover seed production paddocks with BLRV, and in a glasshouse trial it transmitted BLRV from an infected lucerne plant to subterranean clover in a persistent manner.

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Evidence for expression level-dependent modulation of carbohydrate status and viral resistance by the potato leafroll virus movement protein in transgenic tobacco plants

The Plant Journal ◽

10.1046/j.1365-313x.2001.01179.x ◽

2001 ◽

Vol 28 (5) ◽

pp. 529-543 ◽

Cited By ~ 62

Author(s):

Daniel Hofius ◽

Karin Herbers ◽

Michael Melzer ◽

Ayelet Omid ◽

Eckhard Tacke ◽

...

Keyword(s):

Transgenic Tobacco ◽

Movement Protein ◽

Potato Leafroll Virus ◽

Expression Level ◽

Viral Resistance ◽

Virus Movement ◽

Transgenic Tobacco Plants ◽

Tobacco Plants ◽

Leafroll Virus ◽

Carbohydrate Status

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First Report of Bean Leafroll Virus in Chickpea, Lentil, and Dry Pea in Montana

Plant Disease ◽

10.1094/pdis-10-18-1873-pdn ◽

2019 ◽

Vol 103 (5) ◽

pp. 1050-1050

Author(s):

B. Agindotan ◽

J. Fenoglio ◽

M. Mahathar ◽

K. McPhee ◽

M. Burrows

Keyword(s):

First Report ◽

Bean Leafroll Virus ◽

Leafroll Virus

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The Interaction Dynamics of Two Potato Leafroll Virus Movement Proteins Affects Their Localization to the Outer Membranes of Mitochondria and Plastids

Viruses ◽

10.3390/v10110585 ◽

2018 ◽

Vol 10 (11) ◽

pp. 585 ◽

Cited By ~ 8

Author(s):

Stacy DeBlasio ◽

Yi Xu ◽

Richard Johnson ◽

Ana Rebelo ◽

Michael MacCoss ◽

...

Keyword(s):

Mutational Analysis ◽

Affinity Purification ◽

Potato Leafroll Virus ◽

Structural Proteins ◽

Mitochondrial Outer Membrane ◽

Virus Movement ◽

Cell Periphery ◽

Movement Proteins ◽

C Terminus ◽

Leafroll Virus

The Luteoviridae is an agriculturally important family of viruses whose replication and transport are restricted to plant phloem. Their genomes encode for four proteins that regulate viral movement. These include two structural proteins that make up the capsid and two non-structural proteins known as P3a and P17. Little is known about how these proteins interact with each other and the host to coordinate virus movement within and between cells. We used quantitative, affinity purification-mass spectrometry to show that the P3a protein of Potato leafroll virus complexes with virus and that this interaction is partially dependent on P17. Bimolecular complementation assays (BiFC) were used to validate that P3a and P17 self-interact as well as directly interact with each other. Co-localization with fluorescent-based organelle markers demonstrates that P3a directs P17 to the mitochondrial outer membrane while P17 regulates the localization of the P3a-P17 heterodimer to plastids. Residues in the C-terminus of P3a were shown to regulate P3a association with host mitochondria by using mutational analysis and also varying BiFC tag orientation. Collectively, our work reveals that the PLRV movement proteins play a game of intracellular hopscotch along host organelles to transport the virus to the cell periphery.

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