Management of bacterial leaf blight (Xanthomonas axonopodis pv. cymopsidis) of clusterbean in Rajasthan

Here, we report the annotated draft genome sequence of Xanthomonas axonopodis pv. eucalyptorum pathotype strain LPF602 (synonym Xanthomonas axonopodis BSC45a), isolated from eucalypt leaves showing bacterial blight symptoms in Brazil. The availability of these genomic data will help improve the understanding of the evolution and molecular mechanisms involved in the pathogenesis of this microorganism.

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AKTIVITAS ENZIM PEROKSIDASE BAWANG MERAH YANG DIINTRODUKSI DENGAN BAKTERI ENDOFIT DAN TAHAN TERHADAP PENYAKIT HAWAR DAUN BAKTERI (XANTHOMONAS AXONOPODIS PV. ALLII)

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.216131-137 ◽

2016 ◽

Vol 16 (2) ◽

pp. 131

Author(s):

Zurai Resti ◽

Trimurti Habazar ◽

Deddi Prima Putra ◽

Nasrum .

Keyword(s):

Enzyme Activity ◽

Serratia Marcescens ◽

Endophytic Bacteria ◽

Leaf Blight ◽

Bacterial Leaf Blight ◽

Xanthomonas Axonopodis ◽

Defense Enzyme ◽

Peroxidase Enzyme ◽

Induce Resistance ◽

Important Disease

Peroxidase enzyme activity of the introduced shallots with endophytic bacteria and resistant to bacterial leaf blight (Xanthomonas axonopodis pv. allii). Bacterial leaf blight caused by Xanthomonas axonopodis pv. allii is an important disease in shallots. We have earned six isolates of endophytic bacteria, which have the ability to induce systemically resistance to shallots. One mechanism in induce resistance in plants is a change in the plant defense enzyme activity such as peroxidase. The purpose of this study was to calculate the peroxidase enzyme activity of shallots crop is being introduced with endophytic bacteria and is able to induce resistance to Xanthomonas axonopodis pv. allii. This research was conducted by introducing six isolates of endophytic bacteria on shallot bulbs and planted in greenhouse. Shallots crop that was 14 days old then inoculated with the bacterium Xanthomonas axonopodis pv. allii and incubated until symptoms appear. Peroxidase enzyme activity was calculated on the roots and leaves of shallots are 0, 1, 2, 4, 6, 8, 10, 15, and 30 days after inoculation (dai). The results showed an increase in the peroxidase enzyme activity of shallots crop is being introduced by endophytic bacteria compared to the control. Isolates ULG1E2 (Serratia marcescens PPM4) was isolate with the highest peroxidase enzyme activity both in the roots and leaves are 0,051 ìm/ ml.

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Peroxidase Enzyme Activity of Rhizobacteria-introduced Shallots Bulbs to Induce Resistance of Shallot towards Bacterial Leaf Blight (Xanthomonas Axonopodis pv Allii)

Procedia Chemistry ◽

10.1016/j.proche.2015.03.067 ◽

2015 ◽

Vol 14 ◽

pp. 501-507 ◽

Cited By ~ 2

Author(s):

Yulmira Yanti

Keyword(s):

Enzyme Activity ◽

Leaf Blight ◽

Bacterial Leaf Blight ◽

Xanthomonas Axonopodis ◽

Peroxidase Enzyme ◽

Induce Resistance

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Synthesis and Antibacterial Evaluation of Novel 1,3,4-Oxadiazole Derivatives Containing Sulfonate/Carboxylate Moiety

Molecules ◽

10.3390/molecules25071488 ◽

2020 ◽

Vol 25 (7) ◽

pp. 1488

Author(s):

Lei Wang ◽

Xia Zhou ◽

Hui Lu ◽

Xianfu Mu ◽

Linhong Jin

Keyword(s):

Antibacterial Activity ◽

Inhibitory Effect ◽

Sem Analysis ◽

Leaf Blight ◽

Xanthomonas Oryzae ◽

Bacterial Leaf Blight ◽

Xanthomonas Axonopodis ◽

Better Than

In order to discover new lead compounds with high antibacterial activity, a series of new derivatives were designed and synthesized by introducing a sulfonate or carboxylate moiety into the 1,3,4-oxadiazole structure. Antibacterial activity against two phytopathogens, Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas axonopodis pv. citri (Xac), was assayed in vitro. The preliminary results indicated that ten compounds including 4a-1-4a-4 and 4a-11-4a-16 had good antibacterial activity against Xoo, with EC50 values ranging from 50.1-112.5 µM, which was better than those of Bismerthiazol (253.5 µM) and Thiodiazole copper (467.4 µM). Meanwhile, 4a-1, 4a-2, 4a-3 and 4a-4 demonstrated good inhibitory effect against Xanthomonas axonopodis pv. citri with EC50 values around 95.8-155.2 µM which were better than those of bismerthiazol (274.3 µM) and thiodiazole copper (406.3 µM). In addition, in vivo protection activity of compound 4a-2 and 4a-3 against rice bacterial leaf blight was 68.6% and 62.3%, respectively, which were better than bismerthiazol (49.6%) and thiodiazole copper (42.2%). Curative activity of compound 4a-2 and 4a-3 against rice bacterial leaf blight was 62.3% and 56.0%, which were better than bismerthiazol (42.9%) and thiodiazole copper (36.1%). Through scanning electron microscopy (SEM) analysis, it was observed that compound 4a-2 caused the cell membrane of Xanthomonas oryzae pv. oryzae ruptured or deformed. The present results indicated novel derivatives of 5-phenyl sulfonate methyl 1,3,4-oxadiazole might be potential antibacterial agents.

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Phage Biocontrol of Bacterial Leaf Blight Disease on Welsh Onion Caused by Xanthomonas axonopodis pv. allii

Antibiotics ◽

10.3390/antibiotics10050517 ◽

2021 ◽

Vol 10 (5) ◽

pp. 517

Author(s):

Nguyen Thi Thu Nga ◽

Tran Ngoc Tran ◽

Dominique Holtappels ◽

Nguyen Le Kim Kim Ngan ◽

Nguyen Phuoc Hao ◽

...

Keyword(s):

Mekong Delta ◽

Leaf Blight ◽

Field Conditions ◽

Bacterial Leaf Blight ◽

Welsh Onion ◽

Disease Symptoms ◽

Xanthomonas Axonopodis ◽

Phage Cocktail ◽

Significant Yield ◽

Blight Disease

Bacterial leaf blight, which is caused by Xanthomonas axonopodis pv. allii, annually causes significant yield losses to Welsh onion in many producing countries, including Vietnam. In this study, we isolated and characterized lytic phages ɸ16, ɸ17A and ɸ31, specific to X. axonopodis pv. allii and belonging to a new phage species and genus within the Autographiviridae, from four provinces in the Mekong Delta of Vietnam. Moreover, we evaluated their efficacy for the biocontrol of leaf blight in greenhouse and field conditions. When applying the three highly related phages individually or as a three-phage cocktail at 108 PFU/mL in greenhouse conditions, our results show that treatment with ɸ31 alone provides higher disease prevention than the two other phages or the phage cocktail. Furthermore, we compared phage concentrations from 105 to 108 and showed optimal disease control at 107 and 108 PFU/mL. Finally, under field conditions, both phage ɸ31 alone and the phage cocktail treatments suppressed disease symptoms, which was comparable to the chemical bactericide oxolinic acid (Starner). Phage treatment also significantly improved yield, showing the potential of phage as a biocontrol strategy for managing leaf blight in Welsh onion.

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