Peroxidase Enzyme Activity of Rhizobacteria-introduced Shallots Bulbs to Induce Resistance of Shallot towards Bacterial Leaf Blight (Xanthomonas Axonopodis pv Allii)

Peroxidase enzyme activity of the introduced shallots with endophytic bacteria and resistant to bacterial leaf blight (Xanthomonas axonopodis pv. allii). Bacterial leaf blight caused by Xanthomonas axonopodis pv. allii is an important disease in shallots. We have earned six isolates of endophytic bacteria, which have the ability to induce systemically resistance to shallots. One mechanism in induce resistance in plants is a change in the plant defense enzyme activity such as peroxidase. The purpose of this study was to calculate the peroxidase enzyme activity of shallots crop is being introduced with endophytic bacteria and is able to induce resistance to Xanthomonas axonopodis pv. allii. This research was conducted by introducing six isolates of endophytic bacteria on shallot bulbs and planted in greenhouse. Shallots crop that was 14 days old then inoculated with the bacterium Xanthomonas axonopodis pv. allii and incubated until symptoms appear. Peroxidase enzyme activity was calculated on the roots and leaves of shallots are 0, 1, 2, 4, 6, 8, 10, 15, and 30 days after inoculation (dai). The results showed an increase in the peroxidase enzyme activity of shallots crop is being introduced by endophytic bacteria compared to the control. Isolates ULG1E2 (Serratia marcescens PPM4) was isolate with the highest peroxidase enzyme activity both in the roots and leaves are 0,051 ìm/ ml.

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Isolasi, Seleksi, dan Identifikasi Bakteri Endofit sebagai Agens Penginduksi Ketahanan Padi terhadap Hawar Daun Bakteri

Jurnal Fitopatologi Indonesia ◽

10.14692/jfi.12.6.199 ◽

2017 ◽

Vol 12 (6) ◽

pp. 199

Author(s):

Ida Parida ◽

Tri Asmira Damayanti ◽

Giyanto Giyanto

Keyword(s):

Gene Expression ◽

Enzyme Activity ◽

Incubation Period ◽

Endophytic Bacteria ◽

Hypersensitive Reaction ◽

Leaf Blight ◽

Control Measures ◽

Bacterial Leaf Blight ◽

Prolonged Incubation ◽

Induce Resistance

Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae is one of the major problems in rice production in Indonesia. One of the control measures for the disease is by the utilization of endophytic bacteria. This study was aimed to determine the ability of endophytic bacteria isolated from the roots, stems and leaves of rice in inducing plant resistance to bacterial leaf blight on rice. Screening of endophytic bacteria showed that 370 isolates have good viability and have different colony morphology. Among them, 7 isolates were able to induce resistance and 1 isolate was able to promote the growth of rice in the nursery. However, only 8 isolates did not cause hypersensitive reaction on tobacco plants. The selected isolates of endophytic bacteria were further examined for their ability to induce resistance of rice in greenhouse experiments. Observation involved several variables, i.e. PR1 and PBZ1 gene expression, peroxidase enzyme activity, incubation period, and disease progression. Seven isolates of endophytic bacteria were able to induce PR1 and PBZ1 gene expression, 4 isolates were able to increase peroxide enzyme activity, 4 isolates could prolong the incubation period, and 2 isolates can inhibit the development of disease. However, only EB4 451 isolate was consistently able to induce PR1 and PBZ1 gene expression, increased peroxide enzyme activity, prolonged incubation period, and suppressed the progression of the disease. The EB4 451 isolate was identified based on nucleotide sequence as Bacillus subtilis.

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Management of bacterial leaf blight (Xanthomonas axonopodis pv. cymopsidis) of clusterbean in Rajasthan

Indian Phytopathology ◽

10.1007/s42360-020-00299-8 ◽

2021 ◽

Author(s):

S. L. Godara ◽

Narendra Singh

Keyword(s):

Leaf Blight ◽

Bacterial Leaf Blight ◽

Xanthomonas Axonopodis

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Activity of extracellular compounds of Pseudomonas sp. against Xanthomonas axonopodis in vitro and bacterial leaf blight in eucalyptus

Tropical Plant Pathology ◽

10.1590/s1982-56762012000400001 ◽

2012 ◽

Vol 37 (4) ◽

pp. 233-238 ◽

Cited By ~ 15

Author(s):

Lucilene P. Lopes ◽

Admiltom G. Oliveira Jr ◽

Jamile P.O. Beranger ◽

Cíntia G. Góis ◽

Fernanda C.S. Vasconcellos ◽

...

Keyword(s):

Leaf Blight ◽

Bacterial Leaf Blight ◽

Pseudomonas Sp ◽

Xanthomonas Axonopodis

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First Draft Genome Sequence of Xanthomonas axonopodis pv. eucalyptorum, Causal Agent of Bacterial Leaf Blight on Eucalypt

Microbiology Resource Announcements ◽

10.1128/mra.00120-19 ◽

2019 ◽

Vol 8 (16) ◽

Author(s):

Yane F. Neves ◽

Samuel A. Santos ◽

Lúcio M. S. Guimarães ◽

Pedro M. P. Vidigal ◽

Jorge L. Badel ◽

...

Keyword(s):

Genome Sequence ◽

Bacterial Blight ◽

Molecular Mechanisms ◽

Draft Genome ◽

Genomic Data ◽

Leaf Blight ◽

Bacterial Leaf Blight ◽

Draft Genome Sequence ◽

Content Type ◽

Xanthomonas Axonopodis

Here, we report the annotated draft genome sequence of Xanthomonas axonopodis pv. eucalyptorum pathotype strain LPF602 (synonym Xanthomonas axonopodis BSC45a), isolated from eucalypt leaves showing bacterial blight symptoms in Brazil. The availability of these genomic data will help improve the understanding of the evolution and molecular mechanisms involved in the pathogenesis of this microorganism.

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Induce resistance of rice plants against bacterial leaf blight by using salicylic acid application

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/746/1/012003 ◽

2021 ◽

Vol 746 (1) ◽

pp. 012003

Author(s):

W S Suharti ◽

N W A Leana

Keyword(s):

Salicylic Acid ◽

Leaf Blight ◽

Bacterial Leaf Blight ◽

Rice Plants ◽

Induce Resistance

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Synthesis and Antibacterial Evaluation of Novel 1,3,4-Oxadiazole Derivatives Containing Sulfonate/Carboxylate Moiety

Molecules ◽

10.3390/molecules25071488 ◽

2020 ◽

Vol 25 (7) ◽

pp. 1488

Author(s):

Lei Wang ◽

Xia Zhou ◽

Hui Lu ◽

Xianfu Mu ◽

Linhong Jin

Keyword(s):

Antibacterial Activity ◽

Inhibitory Effect ◽

Sem Analysis ◽

Leaf Blight ◽

Xanthomonas Oryzae ◽

Bacterial Leaf Blight ◽

Xanthomonas Axonopodis ◽

Better Than

In order to discover new lead compounds with high antibacterial activity, a series of new derivatives were designed and synthesized by introducing a sulfonate or carboxylate moiety into the 1,3,4-oxadiazole structure. Antibacterial activity against two phytopathogens, Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas axonopodis pv. citri (Xac), was assayed in vitro. The preliminary results indicated that ten compounds including 4a-1-4a-4 and 4a-11-4a-16 had good antibacterial activity against Xoo, with EC50 values ranging from 50.1-112.5 µM, which was better than those of Bismerthiazol (253.5 µM) and Thiodiazole copper (467.4 µM). Meanwhile, 4a-1, 4a-2, 4a-3 and 4a-4 demonstrated good inhibitory effect against Xanthomonas axonopodis pv. citri with EC50 values around 95.8-155.2 µM which were better than those of bismerthiazol (274.3 µM) and thiodiazole copper (406.3 µM). In addition, in vivo protection activity of compound 4a-2 and 4a-3 against rice bacterial leaf blight was 68.6% and 62.3%, respectively, which were better than bismerthiazol (49.6%) and thiodiazole copper (42.2%). Curative activity of compound 4a-2 and 4a-3 against rice bacterial leaf blight was 62.3% and 56.0%, which were better than bismerthiazol (42.9%) and thiodiazole copper (36.1%). Through scanning electron microscopy (SEM) analysis, it was observed that compound 4a-2 caused the cell membrane of Xanthomonas oryzae pv. oryzae ruptured or deformed. The present results indicated novel derivatives of 5-phenyl sulfonate methyl 1,3,4-oxadiazole might be potential antibacterial agents.

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Phage Biocontrol of Bacterial Leaf Blight Disease on Welsh Onion Caused by Xanthomonas axonopodis pv. allii

Antibiotics ◽

10.3390/antibiotics10050517 ◽

2021 ◽

Vol 10 (5) ◽

pp. 517

Author(s):

Nguyen Thi Thu Nga ◽

Tran Ngoc Tran ◽

Dominique Holtappels ◽

Nguyen Le Kim Kim Ngan ◽

Nguyen Phuoc Hao ◽

...

Keyword(s):

Mekong Delta ◽

Leaf Blight ◽

Field Conditions ◽

Bacterial Leaf Blight ◽

Welsh Onion ◽

Disease Symptoms ◽

Xanthomonas Axonopodis ◽

Phage Cocktail ◽

Significant Yield ◽

Blight Disease

Bacterial leaf blight, which is caused by Xanthomonas axonopodis pv. allii, annually causes significant yield losses to Welsh onion in many producing countries, including Vietnam. In this study, we isolated and characterized lytic phages ɸ16, ɸ17A and ɸ31, specific to X. axonopodis pv. allii and belonging to a new phage species and genus within the Autographiviridae, from four provinces in the Mekong Delta of Vietnam. Moreover, we evaluated their efficacy for the biocontrol of leaf blight in greenhouse and field conditions. When applying the three highly related phages individually or as a three-phage cocktail at 108 PFU/mL in greenhouse conditions, our results show that treatment with ɸ31 alone provides higher disease prevention than the two other phages or the phage cocktail. Furthermore, we compared phage concentrations from 105 to 108 and showed optimal disease control at 107 and 108 PFU/mL. Finally, under field conditions, both phage ɸ31 alone and the phage cocktail treatments suppressed disease symptoms, which was comparable to the chemical bactericide oxolinic acid (Starner). Phage treatment also significantly improved yield, showing the potential of phage as a biocontrol strategy for managing leaf blight in Welsh onion.

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