Selection of carrier materials for formulation of the antagonistic Bacillus spp. against rice bacterial leaf blight

Abstract Background Rice leaf blight, which is a devastating disease worldwide, is caused by the bacterium Xanthomonas oryzae pv. oryzae (Xoo). The upregulated by transcription activator-like 1 (UPT) effector box in the promoter region of the rice Xa13 gene plays a key role in Xoo pathogenicity. Mutation of a key bacterial protein-binding site in the UPT box of Xa13 to abolish PXO99-induced Xa13 expression is a way to improve rice resistance to bacteria. Highly efficient generation and selection of transgene-free edited plants are helpful to shorten and simplify the gene editing-based breeding process. Selective elimination of transgenic pollen of T0 plants can enrich the proportion of T1 transgene-free offspring, and expression of a color marker gene in seeds makes the selection of T2 plants very convenient and efficient. In this study, a genome editing and multiplexed selection system was used to generate bacterial leaf blight-resistant and transgene-free rice plants. Results We introduced site-specific mutations into the UPT box using CRISPR/Cas12a technology to hamper with transcription-activator-like effector (TAL) protein binding and gene activation and generated genome-edited rice with improved bacterial blight resistance. Transgenic pollen of T0 plants was eliminated by pollen-specific expression of the α-amylase gene Zmaa1, and the proportion of transgene-free plants increased from 25 to 50% among single T-DNA insertion events in the T1 generation. Transgenic seeds were visually identified and discarded by specific aleuronic expression of DsRed, which reduced the cost by 50% and led to up to 98.64% accuracy for the selection of transgene-free edited plants. Conclusion We demonstrated that core nucleotide deletion in the UPT box of the Xa13 promoter conferred resistance to rice blight, and selection of transgene-free plants was boosted by introducing multiplexed selection. The combination of genome editing and transgene-free selection is an efficient strategy to accelerate functional genomic research and plant breeding.

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Marker-Assisted Selection of Xa21 Conferring Resistance to Bacterial Leaf Blight in indica Rice Cultivar LT2

Rice Science ◽

10.1016/j.rsci.2017.08.004 ◽

2018 ◽

Vol 25 (1) ◽

pp. 52-56 ◽

Cited By ~ 7

Author(s):

Hue Thi Nguyen ◽

Quang Hong Vu ◽

Tan Van Mai ◽

Thu Thi Nguyen ◽

Lam Duc Vu ◽

...

Keyword(s):

Marker Assisted Selection ◽

Indica Rice ◽

Rice Cultivar ◽

Leaf Blight ◽

Bacterial Leaf Blight ◽

Selection Of ◽

Indica Rice Cultivar

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Modification of host–parasite interactions through mutagenesis in quantitative resistance of rice to bacterial leaf blight

The Journal of Agricultural Science ◽

10.1017/s0021859600083258 ◽

1988 ◽

Vol 111 (2) ◽

pp. 309-315 ◽

Cited By ~ 1

Author(s):

H. Nakai ◽

K. Katsumata ◽

M. Gohda ◽

J. Watanabe ◽

K. Koike

Keyword(s):

Quantitative Resistance ◽

Leaf Blight ◽

Bacterial Leaf Blight ◽

Artificial Inoculation ◽

Induced Mutations ◽

Host Parasite Interactions ◽

Group I ◽

Mutant Lines ◽

Host Parasite ◽

Selection Of

SummaryA number of mutant lines showing different levels of improved quantitative resistance to bacterial leaf blight (BLB) were obtained through selection of plants or lines derived from seeds of the rice cultivar Harebare exposed to thermal neutrons and gamma-rays. The selections were made on M2–M4 materials under natural BLB-epidemic conditions and/or by artificial inoculation of one of the BLB isolates. Bacterial isolates predominating under the natural conditions were identified as belonging to the differential BLB isolate group I, and the isolates of this group were used in the artificial inoculation for selection of mutants. The selected M4 mutant lines were tested in M5 for BLB resistance by artificial inoculations of four differential bacterium isolates, groups I, II, III and IV. The M6 mutant lines showed quantitatively elevated resistances to the isolate of bacterium group I and also to the other three differential isolates. Analysis of variance of the data demonstrated that the observed elevated quantitative resistances of the mutants to the isolates I, II, III and IV were isolatespecific. Host-parasite interactions in quantitative resistance to BLB and their modification by induced mutations are discussed.

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Evaluation of Improvement Effect of Restorer Lines on Pyramiding Genes Re-sistant to Rice Blast, Bacterial Leaf Blight and Brown Planthopper

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2016.00031 ◽

2016 ◽

Vol 42 (1) ◽

pp. 31 ◽

Cited By ~ 1

Author(s):

Jue LOU ◽

Wen-Qing YANG ◽

Zhong-Xing LI ◽

Tian-Kuan LUO ◽

Yong-Chu XIE ◽

...

Keyword(s):

Rice Blast ◽

Brown Planthopper ◽

Leaf Blight ◽

Bacterial Leaf Blight ◽

Improvement Effect

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Biogenic silver nanoparticles as an antibacterial agent against bacterial leaf blight causing rice phytopathogen Xanthomonas oryzae pv. oryzae

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-021-02579-7 ◽

2021 ◽

Author(s):

Karunakar Reddy Namburi ◽

Aruna Jyothi Kora ◽

Anuradha Chetukuri ◽

Vijaya Sree Meena Kumari Kota

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Agent ◽

Leaf Blight ◽

Xanthomonas Oryzae ◽

Bacterial Leaf Blight ◽

Biogenic Silver Nanoparticles

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Ginger Essential Oils-Loaded Nanoemulsions: Potential Strategy to Manage Bacterial Leaf Blight Disease and Enhanced Rice Yield

Molecules ◽

10.3390/molecules26133902 ◽

2021 ◽

Vol 26 (13) ◽

pp. 3902

Author(s):

Abdullahi Adamu ◽

Khairulmazmi Ahmad ◽

Yasmeen Siddiqui ◽

Intan Safinar Ismail ◽

Norhayu Asib ◽

...

Keyword(s):

Antibacterial Activity ◽

Electron Microscope ◽

Essential Oils ◽

Disease Severity ◽

Rice Yield ◽

Cell Structure ◽

Leaf Blight ◽

Bacterial Leaf Blight ◽

Control Treatment ◽

Promising Alternative

The bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious rice diseases, causing huge yield losses worldwide. Several technologies and approaches have been opted to reduce the damage; however, these have had limited success. Recently, scientists have been focusing their efforts on developing efficient and environmentally friendly nanobactericides for controlling bacterial diseases in rice fields. In the present study, a scanning electron microscope (SEM), transmission electron microscope (TEM), and a confocal laser scanning microscope (CLSM) were utilized to investigate the mode of actions of ginger EOs on the cell structure of Xoo. The ginger EOs caused the cells to grow abnormally, resulting in an irregular form with hollow layers, whereas the dimethylsulfoxide (DMSO) treatment showed a typical rod shape for the Xoo cell. Ginger EOs restricted the growth and production of biofilms by reducing the number of biofilms generated as indicated by CLSM. Due to the instability, poor solubility, and durability of ginger EOs, a nanoemulsions approach was used, and a glasshouse trial was performed to assess their efficacy on BLB disease control. The in vitro antibacterial activity of the developed nanobactericides was promising at different concentration (50–125 µL/mL) tested. The efficacy was concentration-dependent. There was significant antibacterial activity recorded at higher concentrations. A glasshouse trial revealed that developed nanobactericides managed to suppress BLB disease severity effectively. Treatment at a concentration of 125 μL/mL was the best based on the suppression of disease severity index, AUDPC value, disease reduction (DR), and protection index (PI). Furthermore, findings on plant growth, physiological features, and yield parameters were significantly enhanced compared to the positive control treatment. In conclusion, the results indicated that ginger essential oils loaded-nanoemulsions are a promising alternative to synthetic antibiotics in suppressing Xoo growth, regulating the BLB disease, and enhancing rice yield under a glasshouse trial.

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