Fruit rot of butternut squash (Cucurbita moschata Duch.) caused by Choanephora cucurbitarum in Sri Lanka

`Butterbowl' (NE-RBN-4) is a novel, small-sized (0.8 to 1.36 kg), flavorful (sweet), early maturing (90–95 days), near-oblate butternut type winter squash variety (Cucurbita moschata Duch. Ex Poir). No Butternut squash variety is similar in shape to `Butterbowl'. `Butterbowl' (S6) was derived from selfing a near-oblate open-pollinated S4 line derived from a cross of two true breeding crookneck lines (allelic test) NE-BNCR-67-1-7 (mutant out of `Butternut 23') X golden Cushaw (Agway Co.). Total fruit yield and fruit weight of `Butterbowl' were nearly similar to Butternut `Ponca'. The total fruit weight of'Waltham' was greater than `Butterbowl' in two out of four trials. The vining habit of `Butterbowl' (1.7 to 2.0 m) is more compact than `Waltham' or `Ponca'. `Butterbowl' is suitable for small gardens with limited space due to its compact plant habit. No crookneck fruit developed in `Butterbowl' in all tests. `Butterbowl' is resistant to bacterial spot, black fruit rot, and vine borer while it is moderately susceptible to powdery mildew. `Butterbowl' fruit should be used for consumption up to 45 to 55 days after harvest because slight fruit shriveling occurs at that time due to moisture loss. The fruit cooks uniformally in a microwave oven due to its more uniform flesh thickness.

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Evaluation of Winter Squash and Pumpkin Cultivars for Age-related Resistance to Phytophthora capsici Fruit Rot

HortScience ◽

10.21273/hortsci11173-16 ◽

2016 ◽

Vol 51 (10) ◽

pp. 1251-1255 ◽

Cited By ~ 4

Author(s):

Charles S. Krasnow ◽

Mary K. Hausbeck

Keyword(s):

Cucurbita Pepo ◽

Disease Incidence ◽

Phytophthora Capsici ◽

Genetic Resistance ◽

Fruit Rot ◽

Infested Soil ◽

Cucurbita Moschata ◽

Cucurbita Maxima ◽

Age Related ◽

Winter Squash

Phytophthora capsici annually threatens production of cucurbit and solanaceous crops. Long-lived oospores produced by the pathogen incite primary infection of susceptible plants when conditions are wet. Limiting the rot of winter squash and pumpkin (Cucurbita sp.) fruits is difficult due to the long maturation period when fruits are often in direct contact with infested soil. Genetic resistance to fruit rot is not widely available within Cucurbita sp.; however, age-related resistance (ARR) to P. capsici fruit rot develops in specific cultivars during maturation. The objective of this study was to evaluate the fruits of 12 cultivars of Cucurbita pepo, Cucurbita moschata, and Cucurbita maxima for ARR to P. capsici using a mycelial-plug inoculation method. All Cucurbita pepo and Cucurbita moschata cultivars displayed ARR; 7 days postpollination (dpp) fruits were susceptible, limited lesion development occurred on fruits 22 dpp, and lesions did not develop at 56 dpp. Disease developed on both Cucurbita maxima cultivars tested at 7, 14, 22, and 56 dpp. Firmness of fruit exocarps was measured with a manual penetrometer. Exocarp firmness of all cultivars increased during maturation; however, there was no correlation between firmness and disease incidence among cultivars at 22 dpp (R2 = −0.01, P = 0.85). When fruits of cultivars expressing ARR at 22 dpp were wounded before inoculation, fruit rot developed.

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A PRELIMINARY STUDY ON ESTIMATED GLYCAEMIC INDEX AND MICROSTRUCTURE OF STARCH, IN BOILED CUCURBITA MOSCHATA (SQUASH) FOUND IN SRI LANKA.

International Journal of Advanced Research ◽

10.21474/ijar01/7058 ◽

2018 ◽

Vol 6 (5) ◽

pp. 506-512

Author(s):

AkurangeSujeeviDammadinna Wickramasinghe ◽

◽

AlahendraAcharigeNavinda Kalpitha ◽

PoruthotagePradeepRasika Perera ◽

UdumalagalaGamage Chandrika ◽

...

Keyword(s):

Sri Lanka ◽

Glycaemic Index ◽

Cucurbita Moschata ◽

Preliminary Study

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Screening of Cucurbita moschata Duchesne Germplasm for Crown Rot Resistance to Floridian Isolates of Phytophthora capsici Leonian

HortScience ◽

10.21273/hortsci.46.4.536 ◽

2011 ◽

Vol 46 (4) ◽

pp. 536-540 ◽

Cited By ~ 13

Author(s):

Dario J. Chavez ◽

Eileen A. Kabelka ◽

José X. Chaparro

Keyword(s):

Root Rot ◽

Phytophthora Capsici ◽

Crown Rot ◽

Fruit Rot ◽

Cucurbita Moschata ◽

Breeding Lines ◽

Foliar Blight ◽

Disease Rating ◽

Crown And Root Rot ◽

Highly Virulent

Phytophthora capsici causes seedling death, crown and root rot, fruit rot, and foliar blight on squash and pumpkins (Cucurbita spp. L.). A total of 119 C. moschata accessions, from 39 geographic locations throughout the world, and a highly susceptible butternut squash cultivar, Butterbush, were inoculated with a suspension of three highly virulent P. capsici isolates from Florida to identify resistance to crown rot. Mean disease rating (DR) of the C. moschata collection ranged from 1.4 to 5 (0 to 5 scale with 0 resistant and 5 susceptible). Potential resistant and tolerant individuals were identified in the C. moschata collection. A set of 18 PIs from the original screen were rescreened for crown rot resistance. This rescreen produced similar results as the original screen (r = 0.55, P = 0.01). The accessions PI 176531, PI 458740, PI 442266, PI 442262, and PI 634693 were identified with lowest rates of crown infection with a mean DR less than 1.0 and/or individuals with DR = 0. Further selections from these accessions could be made to develop Cucurbita breeding lines and cultivars with resistance to crown rot caused by P. capsici.

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Cochliobolus tuberculatus. [Descriptions of Fungi and Bacteria].

IMI Descriptions of Fungi and Bacteria ◽

10.1079/dfb/20056401004 ◽

1990 ◽

Author(s):

A. Sivanesan

Keyword(s):

Sri Lanka ◽

Geographical Distribution ◽

Leaf Spot ◽

Fruit Rot

Abstract A description is provided for Cochliobolus tuberculatus. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: Allium, Ananas, Arachis, Bambusa, Brassica, Calendula, Centaurea, Citrus, Cocos, Cyperus, Cosmos, Eichornia, Foeniculum, Juncus, Mangifera, Mentha, Musa, Oryza, Pennisetum, Phlox, Pinus, Psidium, Saccharum, Scirpus, Solanum, Sorghum, Tagetes, Telfairia, Triticum, Vigna, Zea and Zingiber. Also isolated from soil and many other substrata. DISEASE: Leaf spot, storage fruit rot of guava, blight of mango. GEOGRAPHICAL DISTRIBUTION: Egypt, France, India, Indochina, Iraq, Jamaica, Japan, Malaysia, Nigeria, Pakistan, Sri Lanka, Tanzania, USA, USSR. TRANSMISSION: Wind-borne conidia, seed-borne (42, 309; 49, 2848).

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Fruit rot disease in butternut squash caused by Pythium aphanidermatum in Trincomalee district, Sri Lanka

Ceylon Journal of Science ◽

10.4038/cjs.v49i5.7804 ◽

2020 ◽

Vol 49 (5) ◽

pp. 373

Author(s):

P. Sevvel ◽

D. Kugathasan ◽

C. J. Emmanuel

Keyword(s):

Sri Lanka ◽

Pythium Aphanidermatum ◽

Fruit Rot ◽

Rot Disease

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Phenotypic Variability of Ceratocystis paradoxa Isolated from North Western and Western Provinces of Sri Lanka and its Bio Control by Potential Bio-Control Agent; Trichoderma viride

CORD ◽

10.37833/cord.v31i2.61 ◽

2015 ◽

Vol 31 (2) ◽

pp. 10

Author(s):

D.L Jayaratne ◽

M.T.A. Dayarathna

Keyword(s):

Biological Control ◽

Sri Lanka ◽

Cocos Nucifera ◽

Trichoderma Viride ◽

Control Agent ◽

Fruit Rot ◽

Coir Fiber ◽

Western Province ◽

Ceratocystis Paradoxa ◽

North Western

Coconut (Cocos nucifera) is an important economic crop in Sri Lanka; one of the world's largest exporters of coir fiber and coir products. Ceratocystis paradoxa is a pathogen causing stem bleeding, leaf and fruit rot in variety of crop plants including coconut. Since C. paradoxa is associated with coconut trees, there is an opportunity for the pathogen exist in coir and coir products as well. According to the quarantine requirements, this pathogen has to be free in coir and coir products that are exported from Sri Lanka. During this study the pathogen was isolated from the samples of coir collected from different locations in North Western Province and Western Province of Sri Lanka. Colony morphology and spore morphology were compared among these isolates. Bio-control and the sensitivity of the isolates to the antagonist were evaluated against Trichoderma viride. According to the results the pathogen was present in all the samples collected from different locations of the coconut growing triangle. The isolated colonies grown on Potato Dextrose Agar (PDA) plates were white in early stage but became black, one or two days later. The growing colonies produced two asexual spores; the endoconidia and chlamydospores. Endoconidia are formed in the conidiophores making long chains. They are thin-walled cylindrical 9-12 µm x 4.8-7.2 µm in size, yet two isolated from North Western Province had a smaller width of 2.4 µm endoconidia. Chlamydospores are thick walled formed in short chains, dark color, and are mostly oval shaped, 9.6-16.8µm x 7.2-9.6µm in size, yet one isolated from North Western Province had a width of 4.8 µm. The chlamydospores are varied in morphology according to the geographical distribution of the pathogen. The variability of chlamydospore morphology predicts the genomic variability among the isolates. Biological control of C. paradoxa using T. viride was successful in all the isolates used in vitro studies. The experiments showed similar effects of T. viride on all C. paradoxa isolates as the biological control agent by killing the vegetative growth and losing the spore viability.

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First Report of Gliocephalotrichum bulbilium and G. simplex Causing Fruit Rot of Rambutan in Puerto Rico

Plant Disease ◽

10.1094/pdis-02-12-0210-pdn ◽

2012 ◽

Vol 96 (8) ◽

pp. 1225-1225 ◽

Cited By ~ 3

Author(s):

L. M. Serrato-Diaz ◽

E. I. Latoni-Brailowsky ◽

L. I. Rivera-Vargas ◽

R. Goenaga ◽

R. D. French-Monar

Keyword(s):

Puerto Rico ◽

Sri Lanka ◽

Mycelial Growth ◽

Fruit Rot ◽

Its2 Region ◽

Research Station ◽

Diseased Plant ◽

Tubulin Gene ◽

First Report ◽

Β Tubulin

Post-harvest disease losses of rambutan (Nephelium lappaceum L.) have been reported worldwide and several pathogens have been associated with fruit rot (3,4). In 2011, fruit rot of rambutan was observed on 11-year-old trees at the USDA-ARS Tropical Agriculture Research Station in Mayaguez, Puerto Rico. Infected fruit sections (1 mm2) were surface-sterilized, rinsed with sterile deionized-distilled water, and transferred to acidified potato dextrose agar (APDA). Gliocephalotrichum bulbilium J.J. Ellis & Hesseltine (Gb) and G. simplex (J.A. Meyer) B. Wiley & E. Simmons (Gs) were identified using a taxonomic key (1). In corn meal agar (CMA), five isolates of Gb were light yellow-to-light brown. Conidiophores had sterile stipe extensions ranging from 120 to 150 μm long and were produced contiguous to the erect conidiogenous penicilli. Conidia were unicellular, smooth, oblong to elliptical, and 5.5 to 7.5 μm long by 2.0 to 2.5 μm wide. Bulbilloid aggregates were observed and averaged 70 μm long. In CMA, five isolates of Gs were light brown-to-chestnut brown. Conidiophores had sterile stipe extensions 130 to 180 μm long that were produced approximately 15 to 30 μm away from the conidiogenous penicilli. Conidia were unicellular, smooth, cylindrical to elliptical, and with slightly curved ends ranging from 6.5 to 8.5 μm long by 2.0 to 2.5 μm wide. Chlamydospores were unicellular, brown, smooth and thick-walled, averaging 35 μm long. Pathogenicity tests were conducted on five detached fruits per isolate. Five isolates of each Gliocephalotrichum spp. were inoculated on fruits using 5-mm mycelial disks of 8-day-old pure cultures grown in APDA. Untreated controls were inoculated with APDA disks only. Inoculated fruit was kept in a humid chamber for 8 days at 25°C under 12 hours of fluorescent light. Test was repeated once. Five days after inoculation (DAI), white mycelial growth for Gb and golden mycelial growth for Gs were observed on rambutan fruits. Eight DAI, fruit rot, and aril (flesh) rot symptoms were observed on fruits inoculated with isolates of Gb and Gs. Infected fruit changed in color from red to brown, and, on average, mycelia of Gb and Gs covered 50 and 60% of the fruit, respectively. Conidiophores were observed on spintems (hair-like appendages). Control fruit did not rot. Both species were reisolated from diseased plant tissue, thus fulfilling Koch's postulates. For molecular identification of these species of Gliocephalotrichum, the ITS1-5.8S-ITS2 region of the rDNA and a fragment of the β-tubulin gene were amplified by PCR and aligned with other Gb and Gs sequences in NCBI GenBank for comparison. The sequences submitted to GenBank included Gs Accession Nos. JQ688045 and JQ688046 and Gb Accession Nos. JQ688044 and JQ68847 for the ITS sequences. For the β-tubulin gene, Gs Accession Nos. JQ688049 and JQ688050 and Gb Accession Nos. JQ688048 and JQ688051. Both DNA regions had 99.9 to 100% sequence identity to other isolates of Gb and Gs reported in GenBank (1). Gliocephalotrichum spp. have been associated with rambutan fruit rot in Hawaii, Sri Lanka and Thailand (2,4). To our knowledge, this is the first report of G. bulbilium and G. simplex causing fruit rot of rambutan in Puerto Rico. References: (1) C. Decock et al. Mycologia 98:488, 2006. (2) K. A. Nishijima and P. A. Follett. Plant Dis. 86:71, 2002. (3) L. M. Serrato et al. Phytopathology 100:S176, 2010. (4) D. Sivakumar et al. J. Natn. Sci. Coun. Sri Lanka 25:225, 1997.

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