Media optimization using Box Behnken design for enhanced production of biomass, beta-carotene and lipid from Dunaliella salina

The halotolerant microalga Dunaliella salina has been widely studied for natural β-carotene production. This work shows biochemical characterization of three newly isolated Dunaliella salina strains DF15, DF17 and DF40 compared with D. salina CCAP 19/30 (confirmed to be D. tertiolecta) and D. salina UTEX 2538 (also known as D. bardawil). Although all three new strains have been genetically characterized as Dunaliella salina strains, their ability to accumulate carotenoids and their capacity for photoprotection against high light stress are different. DF15 and UTEX 2538 reveal great potential for producing large amount of β-carotene and maintained a high rate of photosynthesis under light of high intensity; however, DF17, DF40 and CCAP 19/30 showed increasing photoinhibition with increasing light intensity, and reduced contents of carotenoids, in particular b-carotene, suggesting that the capacity of photoprotection is dependent on the cellular content of carotenoids, in particular β-carotene. Strong positive correlations were found between the cellular content of each of all-trans β-carotene, 9-cis β-carotene, all-trans α-carotene and zeaxanthin but not lutein in the D. salina strains. Lutein was strongly correlated with respiration in photosynthetic cells and strongly related to photosynthesis, chlorophyll and respiration, suggesting an important and not hitherto identified role for lutein in co-ordinated control of the cellular functions of photosynthesis and respiration in response to changes in light conditions, which is broadly conserved in Dunaliella strains. Statistical analysis based on biochemical data revealed a different grouping strategy from the genetic classification of the strains. The significance of these data for strain selection for commercial carotenoid production is discussed.

Download Full-text

Identification of microRNAs response to high light and salinity that involved in beta-carotene accumulation in microalga Dunaliella salina

Algal Research ◽

10.1016/j.algal.2020.101925 ◽

2020 ◽

Vol 48 ◽

pp. 101925 ◽

Cited By ~ 1

Author(s):

Sulin Lou ◽

Xiulan Zhu ◽

Zhiyong Zeng ◽

Huina Wang ◽

Bin Jia ◽

...

Keyword(s):

Dunaliella Salina ◽

Beta Carotene ◽

High Light

Download Full-text

Effects of phosphorus on the growth and chlorophyll fluorescence of a Dunaliella salina strain isolated from saline soil under nitrate limitation

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale ◽

10.4081/jbr.2016.5866 ◽

2016 ◽

Vol 89 (2) ◽

Cited By ~ 4

Author(s):

Tassnapa Wongsnansilp ◽

Niran Juntawong ◽

Zhe Wu

Keyword(s):

Dunaliella Salina ◽

Beta Carotene ◽

Phosphate Concentration ◽

Carotene Content ◽

Ps Ii ◽

Algae Growth ◽

Kinetics Of ◽

Late Growth ◽

High Phosphate ◽

Northeastern Thailand

An isolated Dunaliella salina (D. salina) KU XI from saline soils in northeastern Thailand was cultured in f/2 medium in column photobioreactor. The variations of the growth, chlorophyll and beta-carotene content and the maximum quantum yield of PS II photochemistry (Fv/Fm) under different NaH2PO4 concentrations were studied. Based on the results, the growth kinetics of D. salina KU XI was established, which could simulate the algae growth rate under different phosphate concentrations and temperatures. The phosphorus could significantly affect the growth and pigments accumulations of this isolated strain. Increasing NaH2PO4 concentration improved the biomass, the total chlorophyll and beta-carotene content, retarded the decrease of Fv/Fm value. The optimal phosphate concentration for the growth of D. salina KU XI was above 72.6 μM. The maximum biomass and beta-carotene were 0.24 g L-1 and 17.4 mg L-1 respectively when NaH2PO4 was 290.4 μM. The algae growth was restrained by phosphate or nitrate when NaH2PO4 below 12.1 μM or above 72.6 μM. It indicated that properly supplementing nitrate in the late growth stage with high phosphate concentration was favored for enhancing the growth and biomass production.

Download Full-text

Enhanced production of polyhydroxybutyrate (PHB) from agro-industrial wastes: fed-batch cultivation and statistical media optimization

Journal of Microbiology Biotechnology and Food Sciences ◽

10.15414/jmbfs.2016.5.6.606-611 ◽

2016 ◽

Vol 05 (06) ◽

pp. 606-611 ◽

Cited By ~ 1

Author(s):

Mahmoud M. Berekaa ◽

Adil M. Al Issa

Keyword(s):

Batch Cultivation ◽

Industrial Wastes ◽

Media Optimization ◽

Fed Batch ◽

Enhanced Production ◽

Fed Batch Cultivation

Download Full-text

Reconstruction and analysis of a carbon-core metabolic network for Dunaliella salina

10.21203/rs.2.9945/v1 ◽

2019 ◽

Author(s):

Melanie Fachet ◽

Carina Witte ◽

Robert J Flassig ◽

Liisa K. Rihko-Struckmann ◽

Zaid McKie-Krisberg ◽

...

Keyword(s):

Metabolic Network ◽

Dunaliella Salina ◽

Beta Carotene ◽

Biological Behavior ◽

Process Understanding ◽

Uptake Rates ◽

Green Microalga ◽

Stoichiometric Network Analysis ◽

Balance Analysis ◽

Carbon Core

Abstract Background The green microalga Dunaliella salina accumulates a high proportion of beta-carotene during abiotic stress conditions. To better understand the intracellular flux distribution leading to carotenoid accumulation, this work aimed at reconstructing a carbon core metabolic network for D. salina CCAP 19/18 based on the recently published. Results The reconstruction resulted in a network model with 221 reactions and 212 metabolites within three compartments: cytosol, chloroplast and mitochondrion. The network was implemented in the MATLAB toolbox CellNetAnalyzer and checked for feasibility. Furthermore, a flux balance analysis was carried out for different light and nutrient uptake rates. The comparison of the experimental knowledge with the model prediction revealed that the results of the stoichiometric network analysis are plausible and in good agreement with the observed behavior. Accordingly, our model provides an excellent new tool to interrogate and better understand the carbon core metabolism of D. salina. Conclusions The reconstructed metabolic network of D. salina presented in this work is able to predict the biological behavior under light and nutrient stress and will lead to an improved process understanding for the optimized production of high-value products in microalgae.

Download Full-text

Synthetic conversion of leaf chloroplasts into carotenoid-rich plastids reveals mechanistic basis of natural chromoplast development

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.2004405117 ◽

2020 ◽

Vol 117 (35) ◽

pp. 21796-21803

Author(s):

Briardo Llorente ◽

Salvador Torres-Montilla ◽

Luca Morelli ◽

Igor Florez-Sarasa ◽

José Tomás Matus ◽

...

Keyword(s):

Morphological Changes ◽

Nuclear Gene ◽

Beta Carotene ◽

Second Stage ◽

Nuclear Gene Expression ◽

Enhanced Production ◽

Plastid Morphology ◽

Threshold Switch ◽

Switch Mechanism ◽

Mechanistic Basis

Plastids, the defining organelles of plant cells, undergo physiological and morphological changes to fulfill distinct biological functions. In particular, the differentiation of chloroplasts into chromoplasts results in an enhanced storage capacity for carotenoids with industrial and nutritional value such as beta-carotene (provitamin A). Here, we show that synthetically inducing a burst in the production of phytoene, the first committed intermediate of the carotenoid pathway, elicits an artificial chloroplast-to-chromoplast differentiation in leaves. Phytoene overproduction initially interferes with photosynthesis, acting as a metabolic threshold switch mechanism that weakens chloroplast identity. In a second stage, phytoene conversion into downstream carotenoids is required for the differentiation of chromoplasts, a process that involves a concurrent reprogramming of nuclear gene expression and plastid morphology for improved carotenoid storage. We hence demonstrate that loss of photosynthetic competence and enhanced production of carotenoids are not just consequences but requirements for chloroplasts to differentiate into chromoplasts.

Download Full-text

Formulation and Statistical Optimization of Culture Medium for Improved Production of Antimicrobial Compound byStreptomycessp. JAJ06

International Journal of Microbiology ◽

10.1155/2013/526260 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 16

Author(s):

Polpass Arul Jose ◽

Kunjukrishnan Kamalakshi Sivakala ◽

Solomon Robinson David Jebakumar

Keyword(s):

Antibiotic Production ◽

Polynomial Equation ◽

Optimization Method ◽

Quadratic Model ◽

Production Medium ◽

Box Behnken Design ◽

Enhanced Production ◽

Burman Design ◽

Plackett Burman Design ◽

Salt Formulation

Streptomycessp. JAJ06 is a seawater-dependent antibiotic producer, previously isolated and characterised from an Indian coastal solar saltern. This paper reports replacement of seawater with a defined salt formulation in production medium and subsequent statistical media optimization to ensure consistent as well as improved antibiotic production byStreptomycessp. JAJ06. This strain was observed to be proficient to produce antibiotic compound with incorporation of chemically defined sodium-chloride-based salt formulation instead of seawater into the production medium. Plackett-Burman design experiment was applied, and three media constituents, starch, KBr, and CaCO3, were recognised to have significant effect on the antibiotic production ofStreptomycesJAJ06 at their individual levels. Subsequently, Response surface methodology with Box-Behnken design was employed to optimize these influencing medium constituents for the improved antibiotic production ofStreptomycessp. JAJ06. A total of 17 experiments were conducted towards the construction of a quadratic model and a second-order polynomial equation. Optimum levels of medium constituents were obtained by analysis of the model and numerical optimization method. When the strain JAJ06 was cultivated in the optimized medium, the antibiotic activity was increased to 173.3 U/mL, 26.8% increase as compared to the original (136.7 U/mL). This study found a useful way to cultivateStreptomycessp. JAJ06 for enhanced production of antibiotic compound.

Download Full-text