Identification of microRNAs response to high light and salinity that involved in beta-carotene accumulation in microalga Dunaliella salina

2020 ◽  
Vol 48 ◽  
pp. 101925 ◽  
Author(s):  
Sulin Lou ◽  
Xiulan Zhu ◽  
Zhiyong Zeng ◽  
Huina Wang ◽  
Bin Jia ◽  
...  
Keyword(s):  
Dunaliella Salina ◽  
Beta Carotene ◽  
High Light

scholarly journals Effects of light intensity and the remaining nitrate concentration on the beta-carotene accumulation of a wild Dunaliella salina strain isolated from the saline soil

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Zhe Wu ◽  
Rokeya Akter ◽  
Wallop Arirob ◽  
Niran Juntawong ◽  
Chunhong Ma ◽  
...  
Keyword(s):  
Light Intensity ◽  
Dunaliella Salina ◽  
Saline Soil ◽  
Nitrate Concentration ◽  
Beta Carotene ◽  
Dry Weight ◽  
High Light Intensity ◽  
High Light ◽  
Northern Thailand ◽  
Cultivation Time

An isolated <em>Dunaliella salina</em> strain from northern Thailand was cultured in modified Johnson’s medium in column photobioreactor. The beta-carotene accumulation mainly depended on the quantities of cells entering into carotenogenesis condition that was significantly enhanced by high started KNO3 concentration. Low remaining nitrate concentration in the culture of each cell (RNCC) was suitable for algae to accumulate beta-carotene. Following the cultivation time extended, RNCC of all cultures decreased and tended to the same level (10-20 pg/cell) although the biomass or betacarotene content in the culture was higher in high started KNO3 concentration. High light intensity restrained the growth especially in low KNO3 concentration but improved betacarotene accumulation and RNCC. The highest biomass and beta-carotene dry weight (DW) were 2.25 g L-1 and 79.2 mg g-1 DW respectively. Above results indicated that increasing the biomass and as early as possible to strengthen the stress on each cell was important to improve the final beta-carotene yield.

The effect of audible sound frequency on the growth and beta-carotene production of Dunaliella salina

2021 ◽  
Vol 141 ◽  
pp. 373-382
Author(s):  
Arezoo Keramati ◽  
Farshid Pajoum Shariati ◽  
Omid Tavakoli ◽  
Zahra Akbari ◽  
Mina Rezaei
Keyword(s):  
Dunaliella Salina ◽  
Beta Carotene ◽  
Sound Frequency ◽  
Audible Sound

scholarly journals Potential of New Isolates of Dunaliella salina for Natural β-Carotene Production

2018 ◽  
Author(s):  
Yanan Xu ◽  
Iskander M. Ibrahim ◽  
Chiziezi I. Wosu ◽  
Ami Ben-Amotz ◽  
Patricia J. Harvey
Keyword(s):  
Dunaliella Salina ◽  
Biochemical Characterization ◽  
Light Stress ◽  
Beta Carotene ◽  
High Rate ◽  
Cellular Functions ◽  
Genetic Classification ◽  
Cellular Content ◽  
Grouping Strategy ◽  
Positive Correlations

The halotolerant microalga Dunaliella salina has been widely studied for natural &beta;-carotene production. This work shows biochemical characterization of three newly isolated Dunaliella salina strains DF15, DF17 and DF40 compared with D. salina CCAP 19/30 (confirmed to be D. tertiolecta) and D. salina UTEX 2538 (also known as D. bardawil). Although all three new strains have been genetically characterized as Dunaliella salina strains, their ability to accumulate carotenoids and their capacity for photoprotection against high light stress are different. DF15 and UTEX 2538 reveal great potential for producing large amount of &beta;-carotene and maintained a high rate of photosynthesis under light of high intensity; however, DF17, DF40 and CCAP 19/30 showed increasing photoinhibition with increasing light intensity, and reduced contents of carotenoids, in particular b-carotene, suggesting that the capacity of photoprotection is dependent on the cellular content of carotenoids, in particular &beta;-carotene. Strong positive correlations were found between the cellular content of each of all-trans &beta;-carotene, 9-cis &beta;-carotene, all-trans &alpha;-carotene and zeaxanthin but not lutein in the D. salina strains. Lutein was strongly correlated with respiration in photosynthetic cells and strongly related to photosynthesis, chlorophyll and respiration, suggesting an important and not hitherto identified role for lutein in co-ordinated control of the cellular functions of photosynthesis and respiration in response to changes in light conditions, which is broadly conserved in Dunaliella strains. Statistical analysis based on biochemical data revealed a different grouping strategy from the genetic classification of the strains. The significance of these data for strain selection for commercial carotenoid production is discussed.

scholarly journals High Light Stress Regimen on Dunaliella Salina Strains For Carotenoids Induction

2016 ◽  
Vol 3 (4) ◽  
Author(s):  
Anh Nguyen ◽  
Dat Tran ◽  
Minh Ho ◽  
Clifford Louime ◽  
Hanh Tran ◽  
...  
Keyword(s):  
Dunaliella Salina ◽  
Light Stress ◽  
High Light ◽  
High Light Stress

scholarly journals Effects of phosphorus on the growth and chlorophyll fluorescence of a Dunaliella salina strain isolated from saline soil under nitrate limitation

2016 ◽  
Vol 89 (2) ◽  
Author(s):  
Tassnapa Wongsnansilp ◽  
Niran Juntawong ◽  
Zhe Wu
Keyword(s):  
Dunaliella Salina ◽  
Beta Carotene ◽  
Phosphate Concentration ◽  
Carotene Content ◽  
Ps Ii ◽  
Algae Growth ◽  
Kinetics Of ◽  
Late Growth ◽  
High Phosphate ◽  
Northeastern Thailand

An isolated <em>Dunaliella salina</em> (<em>D. salina</em>) KU XI from saline soils in northeastern Thailand was cultured in f/2 medium in column photobioreactor. The variations of the growth, chlorophyll and beta-carotene content and the maximum quantum yield of PS II photochemistry (F<sub>v</sub>/F<sub>m</sub>) under different NaH<sub>2</sub>PO<sub>4</sub> concentrations were studied. Based on the results, the growth kinetics of <em>D. salina</em> KU XI was established, which could simulate the algae growth rate under different phosphate concentrations and temperatures. The phosphorus could significantly affect the growth and pigments accumulations of this isolated strain. Increasing NaH<sub>2</sub>PO<sub>4</sub> concentration improved the biomass, the total chlorophyll and beta-carotene content, retarded the decrease of F<sub>v</sub>/F<sub>m</sub> value. The optimal phosphate concentration for the growth of <em>D. salina</em> KU XI was above 72.6 μM. The maximum biomass and beta-carotene were 0.24 g L<sup>-1</sup> and 17.4 mg L<sup>-1</sup> respectively when NaH<sub>2</sub>PO<sub>4</sub> was 290.4 μM. The algae growth was restrained by phosphate or nitrate when NaH<sub>2</sub>PO<sub>4</sub> below 12.1 μM or above 72.6 μM. It indicated that properly supplementing nitrate in the late growth stage with high phosphate concentration was favored for enhancing the growth and biomass production.

scholarly journals Reconstruction and analysis of a carbon-core metabolic network for Dunaliella salina

2019 ◽  
Author(s):  
Melanie Fachet ◽  
Carina Witte ◽  
Robert J Flassig ◽  
Liisa K. Rihko-Struckmann ◽  
Zaid McKie-Krisberg ◽  
...  
Keyword(s):  
Metabolic Network ◽  
Dunaliella Salina ◽  
Beta Carotene ◽  
Biological Behavior ◽  
Process Understanding ◽  
Uptake Rates ◽  
Green Microalga ◽  
Stoichiometric Network Analysis ◽  
Balance Analysis ◽  
Carbon Core

Abstract Background The green microalga Dunaliella salina accumulates a high proportion of beta-carotene during abiotic stress conditions. To better understand the intracellular flux distribution leading to carotenoid accumulation, this work aimed at reconstructing a carbon core metabolic network for D. salina CCAP 19/18 based on the recently published. Results The reconstruction resulted in a network model with 221 reactions and 212 metabolites within three compartments: cytosol, chloroplast and mitochondrion. The network was implemented in the MATLAB toolbox CellNetAnalyzer and checked for feasibility. Furthermore, a flux balance analysis was carried out for different light and nutrient uptake rates. The comparison of the experimental knowledge with the model prediction revealed that the results of the stoichiometric network analysis are plausible and in good agreement with the observed behavior. Accordingly, our model provides an excellent new tool to interrogate and better understand the carbon core metabolism of D. salina. Conclusions The reconstructed metabolic network of D. salina presented in this work is able to predict the biological behavior under light and nutrient stress and will lead to an improved process understanding for the optimized production of high-value products in microalgae.

scholarly journals The Carotenogenic Dunaliella salina CCAP 19/20 Produces Enhanced Levels of Carotenoid under Specific Nutrients Limitation

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Sushanta Kumar Saha ◽  
Naresh Kazipet ◽  
Patrick Murray
Keyword(s):  
Superoxide Dismutase ◽  
Differential Expression ◽  
Dunaliella Salina ◽  
Growth Conditions ◽  
High Light ◽  
Stress Conditions ◽  
Strain Identification ◽  
Superoxide Dismutase 1 ◽  
Combined Nitrogen ◽  
Enzyme Superoxide Dismutase

Dunaliella salina is the popular microalga for β-carotene production. There is still a growing demand for the best strain identification and growth conditions optimization for maximum carotenoids production. Some strains are noncarotenogenic while other strains may respond differently to applied growth conditions and produce enhanced carotenoid levels. This study tested the carotenogenic ability of Dunaliella salina CCAP 19/20 under sixteen stress conditions and certain biochemical changes in response to specific stress were investigated. This study identified the above strain as carotenogenic, which produces maximum carotenoids under high light (240 μmol photons m−2 sec−1) when combined nitrogen and micronutrients (Cu or CuMn) were limited. Based on the intensity of extracted ions chromatograms, lutein (m/z 568.4357) appears as the major carotenoid followed by β-carotene (m/z 536.4446) and α-carotene (m/z 536.4435). A polypeptide of 28.3 kDa appeared while another polypeptide of 25.5 kDa disappeared in stress cells as compared to noncarotenogenic cells. Expression levels of antioxidative-enzyme superoxide dismutase-1 (SOD1, H2O2-resistant) remained identical, while the prominent H2O2-sensitive isoforms SOD2 and SOD3 were downregulated during carotenogenic conditions. Overall, increased carotenoids levels might be due to the response of differential expression of specific polypeptides and retention of H2O2-resistant SOD, which eventually might help the organism to thrive in the tested stress conditions.

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