Isolation and Identification of antifungal metabolite producing endophytic Bacillus subtilis (S17) and its in vitro effect on Colletotrichum falcatum causing red rot in sugarcane

Sugarcane is an important cash crop and used as the chief source of sugar grown in tropical and subtropical regions in India. Sugarcane production is challenged by various biotic and abiotic stresses; among the biotic factors, red rot disease caused by Colletotrichum falcatum is a major disease leading to severe reduction in sugarcane production. Five isolates of C. falcatum were established from red rot infected cane varieties collected from different areas of Uttar Pradesh. Cultural and morphological studies of the isolates were conducted in vitro on oat meal agar (OMA).Variations in their conidial and colony characters of the five isolates were recorded and summerized in this paper.

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Identification and in-vitro assessment of a glycoprotein elicitor isolated from Colletotrichum falcatum: the causal agent of red rot in sugarcane

Pakistan Journal of Botany ◽

10.30848/pjb2021-5(45) ◽

2021 ◽

Vol 53 (5) ◽

Author(s):

Ambreen Khadija Alvi ◽

Javed Iqbal

Keyword(s):

Causal Agent ◽

Red Rot ◽

Colletotrichum Falcatum ◽

In Vitro Assessment

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SCREENING OF SUGARCANE CULTIVARS AGAINST COLLETOTRICHUM FALCATUM CAUSING RED ROT DISEASE AND ITS CONTROL WITH DIFFERENT FUNGICIDES UNDER LABORATORY CONDITIONS

Pakistan Journal of Phytopathology ◽

10.33866/phytopathol.029.01.0381 ◽

2017 ◽

Vol 29 (1) ◽

pp. 103

Author(s):

Muhammad U. Ghazanfar ◽

Waqas Raza ◽

Sharjeel K. Gondal

Keyword(s):

Mycelial Growth ◽

Rating Scale ◽

Fungal Growth ◽

Saccharum Officinarum ◽

Red Rot ◽

Colletotrichum Falcatum ◽

Red Rot Disease ◽

Laboratory Conditions ◽

Rot Disease

Ten sugarcane (Saccharum officinarum L.) cultivars were screened against red rot disease caused by Colletotrichum falcatum under laboratory conditions by artificial inoculation technique. These cultivars were graded under various levels of resistance as well as susceptibility using a standard disease rating scale. Two cultivars i.e. NSG-59 and SPF-244 showed resistant reaction to red rot of sugarcane. Three cultivars i.e. CPF-246, CPF-247 and BF-138 showed moderately resistant (MR) reaction against red rot. Remaining five cultivars showed moderate, susceptible to highly susceptible reaction. The results of in vitro evaluation of seven fungicides at four concentrations (10 ppm, 15 ppm, 20 ppm and 25 ppm) showed that all tested fungicides with all concentrations significantly inhibited the mycelial growth of the pathogen as compared with control. However, the inhibition percentage was increased by increasing the concentrations of tested fungicides. Among fungicides Tilt proved the most effective fungicide by inhibiting linear mycelial growth at all concentrations against of C. falcatum followed Nativo while Metaxyl&Mencozeb was the least effective in terms of retarding fungal growth. The findings of the present study suggested that resistant cultivars may be utilized as a source of resistance and may be more useful as donors in breeding programme aimed at red rot disease resistance and the growth of the pathogen is effected by different concentrations of fungicides may play an important role to manage this disease.

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Over-Expression of Endogenous SUGARWIN Genes Exalted Tolerance against Colletotrichum Infection in Sugarcane

Plants ◽

10.3390/plants10050869 ◽

2021 ◽

Vol 10 (5) ◽

pp. 869

Author(s):

Aqsa Parvaiz ◽

Ghulam Mustafa ◽

Muhammad Sarwar Khan ◽

Muhammad Amjad Ali

Keyword(s):

Transgenic Plants ◽

Fungal Pathogens ◽

Red Rot ◽

Colletotrichum Falcatum ◽

Molecular Tools ◽

Embryogenic Calli ◽

Over Expression ◽

Potential Source ◽

First Time

Sugarcane being the major contributor of sugar and potential source of biofuel around the globe, occupies significant commercial importance. Red rot is the most devastating disease of sugarcane, severely affecting its quality as well as yield. Here we report the overexpression of SUGARWIN1 and SUGARWIN2 genes in any field crop for the first time. For this purpose, SUGAWIN1 and SUGARWIN2 were cloned downstream of maize ubiquitin (Ubi-1) promoter to construct two independent expression cassettes. The bar gene conferring resistance against phosphinothricin was used as selectable marker. Embryogenic calli of sugarcane were bombarded with both expression cassettes and selected on regeneration medium supplemented with phosphinothricin. The phosphinothricin-resistant shoots were rooted and then, analyzed using molecular tools at the genomic as well as transcriptomic levels. The transcriptomic analysis, using real time qPCR, showed that expression of SUGARWIN1 (SWO) and SUGARWIN2 (SWT) was higher in transgenic plants as compared to untransformed plants. Our results further demonstrated that over expression of these genes under maize ubiquitin (Ubi-1) promoter causes significant restriction in proliferation of red rot causal agent, Colletotrichum falcatum in sugarcane transgenic plants, under in vitro conditions. This report may open up exciting possibilities to extend this technology to other monocots for the development of crops with better ability to withstand fungal pathogens.

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