Virulence potential of two entomopathogenic nematodes, their associated bacteria, and its metabolites to larvae of Pieris brassicae L. (Lepidoptera, Pieridae) in cabbage under greenhouse and field bioassays

2021 ◽  
Author(s):  
Waseem Abbas ◽  
Nazir Javed ◽  
Imran-ul Haq ◽  
Sohail Ahmed
Keyword(s):  
Entomopathogenic Nematodes ◽  
Pieris Brassicae ◽  
Associated Bacteria ◽  
Virulence Potential

Production and Infectivity of Some Entomopathogenic Nematodes Against Larvae and Pupae of Cabbage Butterfly, Pieris brassicae L. (Lepidoptera:Pieridae)

2004 ◽  
Vol 2 (1) ◽  
pp. 86-91 ◽  
Author(s):  
A.N. Mahar ◽  
N.D. JAN ◽  
Q.I. Chachar ◽  
G.S. Markhand ◽  
M. Munir ◽  
...  
Keyword(s):  
Entomopathogenic Nematodes ◽  
Pieris Brassicae ◽  
Cabbage Butterfly

scholarly journals Bio-efficacy of entomopathogenic nematodes, Steinernema feltiae and Heterorhabditis bacteriophora against the Cabbage butterfly Pieris brassicae (L.), under laboratory conditions

2021 ◽  
Author(s):  
Kasi Indra Kumar ◽  
Mohinder Singh ◽  
Kanchhi Maya Waiba ◽  
Sharma Monika ◽  
MA Waseem ◽  
...  
Keyword(s):  
Larvicidal Activity ◽  
Entomopathogenic Nematodes ◽  
Insect Pests ◽  
Larval Mortality ◽  
Petri Dish ◽  
Pieris Brassicae ◽  
Steinernema Feltiae ◽  
Pupal Development ◽  
Cabbage Butterfly ◽  
Cabbage Pests

Abstract BACKGROUND: Keeping in view the serious health and environmental apprehensions associated with the use of pesticides, entomopathogenic nematodes have the potential to supersede larvicidal activity for the management of various insect pests. RESULT: The lab experiments were conducted to test the pathogenicity of two EPNs species S. feltiaeand H. bacteriophora at different (IJs/cm2) concentrations against cabbage pests. Based on the pathogenicity of strains, only two isolates effectively show larvicidal activity. The native isolate was obtained from soil samples, collected from Rajgarh, Hamachi Pradesh, India. Petri dish bioassay use nematodes S. feltiae HR1 and H. bacteriophora HR2 species dose (0, 10, 20, 40, 80, 160/ IJs/cm2). The highest (%) 2nd instar larval mortality was recorded in treatments with H. bacteriophora and S. feltiae @ 160 /IJs/cm-2 were (72.08, 67.42 percent). And 4th instar larval mortality was recorded in treatment with H. bacteriophora, and S. feltiae @ 160 /IJs/cm-2 were (85.38,69.50 percent). The next best treatments in order of their efficacy’s pupae mortality were H. bacteriophora and S. feltiae @ 160 /IJs/cm-2 (74.12, percent) both are seam result, H. bacteriophora and S. feltiae @ 80 /IJs/cm-2 (62.12, 58.58 percent). Larvicidal activity after 48- and 72-hours exposure, the S. feltiae and H. bacteriophora (1.0, 1.30, 1.60, 1.90, 2.20 /IJs/cm2) showed potent larvicidal activity with LC50, LC75 and LC90 of all instars and pupae show high mortality. The strain inhibits the larval and pupal development 48 to 72 hr exposer time with LC50 range from 11.30 to 39.94, LC75 18.15 to 73.54, LC90 61.80 to 99.21.CONCLUSION: These studies demonstrate the challenge for cabbage butterfly P. brassicae. The local indigenous strains of EPNs (S. feltiae HR1, H. bacteriophora HR2) as a good biocontrol agent against, cruciferous vegetables crop pest P. brassicae.

scholarly journals Microbial Biopesticides in Agroecosystems

Agronomy ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 235 ◽  
Author(s):  
Luca Ruiu
Keyword(s):  
Entomopathogenic Nematodes ◽  
Current Knowledge ◽  
Global Market ◽  
Associated Bacteria ◽  
Microbial Species ◽  
Bacterium Bacillus ◽  
Different Strains ◽  
Species Specific ◽  
Invertebrate Pests ◽  
Active Substances

Microbial biopesticides include several microorganisms like bacteria, fungi, baculoviruses, and nematode-associated bacteria acting against invertebrate pests in agro-ecosystems. The biopesticide sector is experiencing a significant growth and many discoveries are being developed into new biopesticidal products that are fueling a growing global market offer. Following a few decades of successful use of the entomopathogenic bacterium Bacillus thuringiensis and a few other microbial species, recent academic and industrial efforts have led to the discovery of new microbial species and strains, and of their specific toxins and virulence factors. Many of these have, therefore, been developed into commercial products. Bacterial entomopathogens include several Bacillaceae, Serratia, Pseudomonas, Yersinia, Burkholderia, Chromobacterium, Streptomyces, and Saccharopolyspora species, while fungi comprise different strains of Beauveria bassiana, B. brongniartii, Metarhizium anisopliae, Verticillium, Lecanicillium, Hirsutella, Paecilomyces, and Isaria species. Baculoviruses are species-specific and refer to niche products active against chewing insects, especially Lepidopteran caterpillars. Entomopathogenic nematodes (EPNs) mainly include species in the genera Heterorhabditis and Steinernema associated with mutualistic symbiotic bacteria belonging to the genera Photorhabdus and Xenorhabdus. An updated representation of the current knowledge on microbial biopesticides and of the availability of active substances that can be used in integrated pest management programs in agro-ecosystems is reported here.

scholarly journals Bio-efficacy of entomopathogenic nematodes, Steinernema feltiae and Heterorhabditis bacteriophora against the Cabbage butterfly (Pieris brassicae [L.]) under laboratory conditions

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Indra Kumar Kasi ◽  
Mohinder Singh ◽  
Kanchhi Maya Waiba ◽  
S. Monika ◽  
M. A. Waseem ◽  
...  
Keyword(s):  
Larvicidal Activity ◽  
Entomopathogenic Nematodes ◽  
Insect Pests ◽  
Larval Mortality ◽  
Petri Dish ◽  
Heterorhabditis Bacteriophora ◽  
Pieris Brassicae ◽  
Steinernema Feltiae ◽  
Cabbage Butterfly ◽  
Local Species

Abstract Background Entomopathogenic nematodes (EPNs) have the potential to supersede larvicidal activity for the management of various insect pests. Result Lab experiments were conducted to test the pathogenicity of 2 EPNs local species; Steinernema feltiae and Heterorhabditis bacteriophora at different (IJs/cm2) concentrations against the cabbage butterfly, Pieris brassicae (L.). The native isolate was obtained from soil samples, collected from Rajgarh, Hamachi Pradesh, India. Petri dish bioassay used the EPNs species (S. feltiae HR1 and H. bacteriophora HR2) at the concentrations (0, 10, 20, 40, 80, 160 IJs/cm2). Based on the pathogenicity of the strains, only 2 isolates effectively showed larvicidal activity. The highest (%) (72.08 and 67.42%), at the 2nd instar larval mortality was recorded in the treatments with H. bacteriophora and S. feltiae at160 IJs/cm2, respectively. At the 4th instar larvae, respective larval mortality (85.38, 69.50%) was recorded in treatment with H. bacteriophora, and S. feltiae, respectively, at160 IJs/cm2. In case of pupae, the mortality rates were (62.12, 58.58%) for H. bacteriophora and S. feltiae, respectively, at 160 IJs/cm2; (74 and 12%) for both the tested EPNs, respectively, at 80 IJs/cm2. Percent of P. brassicae larval mortality treated with the tested EPN isolates was significantly higher than the untreated control. Results revealed that the percent of larval mortality significantly increased with the increase in time periods, being maximum at 72 h. S. feltiae and H. bacteriophora, strains showed potent larvicidal activity at low concentration even at 48 and 72 h of exposure. Conclusion This study revealed that the local strains of EPNs (S. feltiae HR1 and H. bacteriophora HR2) were found as a biocontrol agent against P. brassicae.

Entomopathogenic Nematodes of Thrips Thripinema spp. (Nematoda: Tylenchida: Allantonematidae)

EDIS ◽  
2017 ◽  
Vol 2017 (5) ◽  
pp. 4
Author(s):  
Danielle M. Sprague ◽  
Joseph E. Funderburk
Keyword(s):  
Life Cycle ◽  
Entomopathogenic Nematodes ◽  
Economic Importance

Originally published on the Featured Creatures Website at http://entnemdept.ufl.edu/creatures/nematode/Thripinema_spp.htm Includes: Introduction - Distribution - Life Cycle and Biology - Symptoms - Hosts and Identification - Economic Importance - Management - Selected References

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