Characterization of adriamycin-resistant and radiation-sensitive Chinese hamster cell lines

1992 ◽  
Vol 44 (9) ◽  
pp. 1859-1868 ◽  
Author(s):  
Marguerite A. Sognier ◽  
Zhang Yin ◽  
Richard L. Eberle ◽  
James A. Belli
Keyword(s):  
Cell Lines ◽  
Chinese Hamster ◽  
Chinese Hamster Cell

Construction and characterization of Chinese hamster cell EmtA EmtB double mutants

1983 ◽  
Vol 3 (5) ◽  
pp. 761-772
Author(s):  
S Chang ◽  
J J Wasmuth
Keyword(s):  
Ribosomal Protein ◽  
Cell Lines ◽  
Ribosomal Proteins ◽  
Hybrid Cell ◽  
Polyacrylamide Gel Electrophoresis ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Altered Form ◽  
Hybrid Cell Lines

Starting with hybrid cell lines between a Chinese hamster cell EmtA mutant and a Chinese hamster cell EmtB mutant, we have constructed cell lines that are homozygous for mutant alleles at both the emtA locus and the emtB locus, by using a two-step segregation protocol. The EmtA EmtB double mutants are approximately 10-fold more resistant to emetine inhibition than either of the parental mutants. Having both the EmtA mutation and the EmtB mutation expressed in the same cell also results in a level of resistance to cryptopleurine that is significantly higher than a simple additive effect of the two mutations alone. Analysis of ribosomal proteins by two-dimensional polyacrylamide gel electrophoresis demonstrated that a parental hybrid and a first-step segregant, which has lost the wild-type emtA allele, synthesize both a normal and an altered form of ribosomal protein S14, whereas an EmtA EmtB double mutant synthesizes only the altered form of this ribosomal protein. This result confirms that the emtB locus is the structural gene for ribosomal protein S14. Our results also suggest that the products of the emtA and emtB loci interact directly, indicating that the emtA locus, like the emtB locus, encodes a component of the ribosome.

scholarly journals Construction and characterization of Chinese hamster cell EmtA EmtB double mutants.

1983 ◽  
Vol 3 (5) ◽  
pp. 761-772 ◽  
Author(s):  
S Chang ◽  
J J Wasmuth
Keyword(s):  
Ribosomal Protein ◽  
Cell Lines ◽  
Ribosomal Proteins ◽  
Hybrid Cell ◽  
Polyacrylamide Gel Electrophoresis ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Altered Form ◽  
Hybrid Cell Lines

Starting with hybrid cell lines between a Chinese hamster cell EmtA mutant and a Chinese hamster cell EmtB mutant, we have constructed cell lines that are homozygous for mutant alleles at both the emtA locus and the emtB locus, by using a two-step segregation protocol. The EmtA EmtB double mutants are approximately 10-fold more resistant to emetine inhibition than either of the parental mutants. Having both the EmtA mutation and the EmtB mutation expressed in the same cell also results in a level of resistance to cryptopleurine that is significantly higher than a simple additive effect of the two mutations alone. Analysis of ribosomal proteins by two-dimensional polyacrylamide gel electrophoresis demonstrated that a parental hybrid and a first-step segregant, which has lost the wild-type emtA allele, synthesize both a normal and an altered form of ribosomal protein S14, whereas an EmtA EmtB double mutant synthesizes only the altered form of this ribosomal protein. This result confirms that the emtB locus is the structural gene for ribosomal protein S14. Our results also suggest that the products of the emtA and emtB loci interact directly, indicating that the emtA locus, like the emtB locus, encodes a component of the ribosome.

Assessment of Persisting Chromosome Aberrations by Flow Karyotyping of Cloned Chinese Hamster Cells

1988 ◽  
Vol 43 (11-12) ◽  
pp. 948-954 ◽  
Author(s):  
Friedrich J. Otto
Keyword(s):  
Flow Cytometry ◽  
High Resolution ◽  
Cell Lines ◽  
Chromosomal Aberrations ◽  
Chromosome Aberrations ◽  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
Cell Clones ◽  
Permanent Cell

Abstract A preparation, staining and measuring protocol for high resolution flow cytometry of chromosomes was developed. This method allows us to identify all chromosome types and is suited for characterization of permanent cell lines and cell clones by establishing their flow karyotypes. In cell clones this procedure can be used for the detection of chromosomal aberrations which appear spontaneously or are induced by mutagen treatment and persist in the cell population.

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