A computer simulation program for the study of cellular growth kinetics and its application to the analysis of human lymphoma cells in vitro

1975 ◽  
Vol 8 (2) ◽  
pp. 118-128 ◽  
Author(s):  
Charles E. Donaghey ◽  
Benjamin Drewinko
Keyword(s):  
Computer Simulation ◽  
Growth Kinetics ◽  
Cellular Growth ◽  
Simulation Program ◽  
Lymphoma Cells ◽  
Computer Simulation Program ◽  
Human Lymphoma

Modeling of multi-electrolyte transport in charged ceramic and organic nanofilters using the computer simulation program NanoFlux

Desalination ◽  
2002 ◽  
Vol 147 (1-3) ◽  
pp. 231-236 ◽  
Author(s):  
J. Palmeri ◽  
J. Sandeaux ◽  
R. Sandeaux ◽  
X. Lefebvre ◽  
P. David ◽  
...  
Keyword(s):  
Computer Simulation ◽  
Electrolyte Transport ◽  
Simulation Program ◽  
Computer Simulation Program

Effect of Cutter Performance on Finished Tooth Form in Gear Shaving

1992 ◽  
Author(s):  
I. Moriwaki ◽  
M. Fujita
Keyword(s):  
Computer Simulation ◽  
Mean Value ◽  
Cutting Performance ◽  
Simulation Program ◽  
Tooth Surface ◽  
Depth Of Cut ◽  
Small Depth ◽  
Computer Simulation Program ◽  
Tooth Form ◽  
Cutting Model

Abstract The authors have been developed a computer simulation program of gear shaving. In the present paper, a new cutting model of shaving process is proposed so as to incorporate an effect of a cutting performance of shaving cutter into the simulation program. In this cutting model, it is assumed that a tooth flank material of work gear can be removed only when a depth of cut of a cutting edge exceeds a certain criterion. It is also assumed that the criterion have no definite value but has the nominal distribution over the tooth surface. The mean value of the distribution can define a characteristic of cutting performance of shaving cutter. The small mean value means that even small depth of cut can cause a stock removal; i.e. a good cutter performance. The computer simulations on gear shaving are performed to reveal the effect of the cutter performance on shaved tooth form. Under the conditions used in these simulations, the pressure angle error on the shaved tooth profile becomes remarkable as the cutter performance becomes worse. Thus, the developed computer simulation program of gear shaving has a reliability on the prediction of shaved tooth form. It will be useful for design of shaving cutter, judgement of tool life, and so on.

The Use of Computer Simulation of Merged Variation to Predict Rework Levels on Ship's Hull Blocks

1988 ◽  
Vol 4 (03) ◽  
pp. 155-168
Author(s):  
R.L. Storch ◽  
P.J. Giesy
Keyword(s):  
Computer Simulation ◽  
Probability Distribution ◽  
Subsequent Development ◽  
Simulation Method ◽  
Simulation Program ◽  
Modular Construction ◽  
Accuracy Control ◽  
Probability Curve ◽  
Productivity Losses ◽  
Computer Simulation Program

In the modular construction of ships, significant productivity losses can occur during the erection stage, when the modules, or hull blocks, are joined together. Frequently, adjacent blocks do not fit together properly, and rework of one or both of the mating block interfaces is necessary to correct the problem. The specific cause of rework is the variation of plate edges at the block interface, which is itself a cumulative product of numerous manufacturing variations inherent in hull block construction. Variation in manufacturing is unavoidable, but not uncontrollable. The application of accuracy control techniques in shipbuilding has proven that a statistical analysis of variation makes possible an accurate prediction of its effects. This paper presents an examination of block interface variation, and the subsequent development of a computer simulation method of predicting rework levels on those blocks. The complex interaction of all the edges' random variations at the block interface gives rise to a unique rework probability distribution. This probability distribution is evaluated by means of the computer simulation program, which provides estimates of the average rework anticipated, the shape of the probability curve, and other parameters. Similar predictions are also available for cost and labor of required rework. In addition to predicting rework levels, the simulation program can be a useful tool for reducing those levels.

scholarly journals Adhesion to high endothelial venules: a model for dissemination mechanisms in non-Hodgkin's lymphoma

Blood ◽  
1993 ◽  
Vol 82 (1) ◽  
pp. 262-267 ◽  
Author(s):  
R Stauder ◽  
S Hamader ◽  
B Fasching ◽  
G Kemmler ◽  
J Thaler ◽  
...  
Keyword(s):  
Hodgkin’S Lymphoma ◽  
Lymphocytic Leukemia ◽  
Hodgkin's Lymphoma ◽  
Lymphoma Cells ◽  
High Endothelial Venules ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma ◽  
Human Lymphoma ◽  
Binet Stage

The interaction of human lymphoma cells with high endothelial venules (HEVs) on sections of lymphatic tissues was studied in 44 cases of non- Hodgkin's lymphoma (NHL) with the in vitro HEV binding assay. The relative adherence ratio (RAR) of lymphoma cells to HEVs as related to that of reactive lymphocytes was 0.29 to 4.64 in 38 cases of B chronic lymphocytic leukemia (CLL), 1.15 and 1.54 in two cases of immunocytic NHL, 1.12 and 0.70 in two cases of centrocytic NHL, 1.98 in one case of a peripheral T-NHL, whereas plasma cell leukemia cells adhered very weakly (RAR 0.1). Among the patients suffering from CLL a pronounced HEV binding ability of tumor cells correlated significantly with the more unfavorable Binet stages B and C (median 1.32) as well as with a widespread lymphatic dissemination, which strongly indicates a hematogenous, HEV-mediated spread (median 1.34). In contrast, weak adherence to HEVs was associated with Binet stage A (median 0.85; P < .05) and with a lacking or only localized clinical involvement of lymph nodes (median 0.84; P < .01). Thus, specific HEV recognition processes even operate in lymphoid neoplasms and via this mechanism seem to influence the dissemination of tumors.

scholarly journals Label-free impedance flow cytometry for nanotoxicity screening

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Melanie Ostermann ◽  
Alexander Sauter ◽  
Ying Xue ◽  
Eivind Birkeland ◽  
Julia Schoelermann ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Human Health ◽  
Trypan Blue ◽  
Label Free ◽  
Trigger Level ◽  
Lymphoma Cells ◽  
Human Lymphoma ◽  
Buffer Composition ◽  
Cost Efficient

AbstractThe development of reliable and cost-efficient methods to assess the toxicity of nanomaterials (NMs) is critical for the proper identification of their impact on human health and for ensuring a safe progress of nanotechnology. In this study, we investigated the reliability and applicability of label-free impedance flow cytometry (IFC) for in vitro nanotoxicity screening, which avoids time-consuming labelling steps and minimizes possible NM-induced interferences. U937 human lymphoma cells were exposed for 24 h to eight different nanomaterials at five concentrations (2, 10, 20, 50, and 100 μg/mL). The NMs’ effect on viability was measured using IFC and the results were compared to those obtained by trypan blue (TB) dye exclusion and conventional flow cytometry (FC). To discriminate viable from necrotic cells, the IFC measurement settings regarding signal trigger level and frequency, as well as the buffer composition, were optimised. A clear discrimination between viable and necrotic cells was obtained at 6 MHz in a sucrose-based measurement buffer. Nanomaterial-induced interferences were not detected for IFC. The IFC and TB assay results were in accordance for all NMs. The IFC was found to be robust, reliable and less prone to interferences due to the advantage of being label-free.

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