Correlative retention time peak identification method for glycated haemoglobin in high-performance liquid chromatography

1994 ◽  
Vol 661 (1-2) ◽  
pp. 143-151 ◽  
Author(s):  
Masahito Ito ◽  
Junkichi Miura ◽  
Mitsuo Ito ◽  
Fuminori Umesato ◽  
Kenji Yasuda ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Retention Time ◽  
High Performance ◽  
Glycated Haemoglobin ◽  
Identification Method ◽  
Peak Identification

scholarly journals The Cytology, Isozyme, HPLC Fingerprint, and Interspecific Hybridization Studies of Genus Epimedium (Berberidaceae)

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Lin-Jiao Wang ◽  
Mao-Yin Sheng
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Interspecific Hybridization ◽  
Retention Time ◽  
High Performance ◽  
Symmetry Type ◽  
Hplc Fingerprint ◽  
C Banding ◽  
Eleven Species

104 samples from 27 accessions belonging to 12 species of genus Epimedium were studied on the basis of cytology observation, POD (i.e., peroxide) isozyme, high performance liquid chromatography (i.e., HPLC) fingerprint, and interspecific hybridization. The cytology observation showed karyotypes of twelve species studied; all are 2A symmetry type of Stebbins standard and similar to each other, and except for karyotype of E. leptorrhizum which is 2n = 2x = 8m (2SAT) + 4sm, the rest are 2n = 2x = 6m (2SAT) + 6sm. Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands. Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others. Studies on the HPLC fingerprint showed that the HPLC fingerprint of different species has characteristic peaks, divided into two regions (retention time < 10 min and retention time > 10 min). Results of interspecific hybridization showed that crosses of any combination among seven species studied are successful and the rates of grain set vary greatly. Based on these results, the system and phylogeny of this genus were inferred.

scholarly journals Glycated Haemoglobin: An Assessment of High Capacity Liquid Chromatographic and Immunoassay Methods

1992 ◽  
Vol 29 (5) ◽  
pp. 494-505 ◽  
Author(s):  
Susan J Standing ◽  
Richard P Taylor
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Throughput ◽  
High Performance ◽  
High Capacity ◽  
Glycated Haemoglobin ◽  
Coefficients Of Variation ◽  
Operation Flexibility ◽  
Liquid Chromatographic

We have assessed five high-throughput systems for the measurement of glycated haemoglobin and have reviewed published evaluations of individual analysers. All systems offered better precision than a widely used electroendosmosis method. The low pressure chromatography and immunoassay systems demonstrated greater between-batch imprecision than the high performance liquid chromatography analysers, the latter achieving the proposed analytical goal of between-batch coefficients of variation less than 5%. Agreement between all systems measuring HbA1 was good but there was variability amongst observed HbA1c values. The systems were also assessed for their quality of chromatographic separation, simplicity of operation, flexibility, cost and potential for interference by other haemoglobins.

Artificial Immune Network in the Recognition of Yunnan Herbal Medicine

2013 ◽  
Vol 718-720 ◽  
pp. 2353-2358
Author(s):  
Chao Huang ◽  
Jin He ◽  
Ji Yong Zhao
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Herbal Medicine ◽  
High Performance ◽  
Immune Network ◽  
Artificial Immune ◽  
Identification Method ◽  
Artificial Immune Network ◽  
Recognition Ability

Concerning simple and unscientific of Yunnan herbal medicine identification method, a kind of identification method based on Artificial Immune Network was proposed in this paper. Using high performance liquid chromatography extracts fingerprints from Yunnan herbal medicine,and then the data of fingerprints were trained by the Artificial Immune Network. In comparison with the traditional K-means algorithm,the experiment results show that Artificial Immune Network has higher classification and recognition ability.

Characterization and distribution of bombesin-like peptides in the rat brain and gastrointestinal tract

1990 ◽  
Vol 68 (9) ◽  
pp. 1142-1145 ◽  
Author(s):  
Angel Hernanz
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Gastrointestinal Tract ◽  
Rat Brain ◽  
Retention Time ◽  
High Performance ◽  
Reverse Phase ◽  
Whole Brain ◽  
Gastrin Releasing Peptide ◽  
Peptide Peak

Extracts of rat brain and gastrointestinal tract, analyzed by reverse-phase high-performance liquid chromatography and radioimmunoassay, contained two bombesin-like immunoreactivity peaks with similar retention times as porcine gastrin-releasing peptide (GRP) and its COOH-terminal decapeptide, neuromedin C or GRP(18–27). However, the GRP-like peptide peak did not elute with exactly the same retention time as porcine GRP. The highest concentration of bombesin-like immunoreactivity was found in extracts of antrum, whereas the lowest was found in whole brain. Neuromedin C was present at lower concentrations than the GRP in antrum, duodenum, and ileum, while similar amounts of each were found in brain.Key words: gastrin-releasing peptide, neuromedin C, rat, brain, intestine.

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