scholarly journals The Cytology, Isozyme, HPLC Fingerprint, and Interspecific Hybridization Studies of Genus Epimedium (Berberidaceae)

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Lin-Jiao Wang ◽  
Mao-Yin Sheng
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Interspecific Hybridization ◽  
Retention Time ◽  
High Performance ◽  
Symmetry Type ◽  
Hplc Fingerprint ◽  
C Banding ◽  
Eleven Species

104 samples from 27 accessions belonging to 12 species of genus Epimedium were studied on the basis of cytology observation, POD (i.e., peroxide) isozyme, high performance liquid chromatography (i.e., HPLC) fingerprint, and interspecific hybridization. The cytology observation showed karyotypes of twelve species studied; all are 2A symmetry type of Stebbins standard and similar to each other, and except for karyotype of E. leptorrhizum which is 2n = 2x = 8m (2SAT) + 4sm, the rest are 2n = 2x = 6m (2SAT) + 6sm. Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands. Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others. Studies on the HPLC fingerprint showed that the HPLC fingerprint of different species has characteristic peaks, divided into two regions (retention time < 10 min and retention time > 10 min). Results of interspecific hybridization showed that crosses of any combination among seven species studied are successful and the rates of grain set vary greatly. Based on these results, the system and phylogeny of this genus were inferred.

scholarly journals Authentication of the Bilberry Extracts by an HPLC Fingerprint Method Combining Reference Standard Extracts

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2514
Author(s):  
Bingbing Liu ◽  
Tiantian Hu ◽  
Weidong Yan
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Reference Standard ◽  
Black Rice ◽  
Hplc Fingerprint ◽  
Berry Extracts ◽  
Bilberry Extract

A simple and fast high-performance liquid chromatography (HPLC) fingerprint method combining reference standard extract for the identification of bilberry extract was developed and validated. Six batches of bilberry extract collected from different manufactures were used to establish the HPLC fingerprint. Other berry extracts—such as blueberry extracts, mulberry extracts, cranberry extracts, and black rice extracts—were also analyzed for their HPLC chromatograms. The fingerprints of five batches of bilberry extract showed high similarities, while one batch was distinguished from others. Additionally, the content of anthocyanin Cyanidin-3-O-glucoside (Cy-3-glc) in each berry extract was analyzed and compared. The results indicate that this HPLC fingerprint method, combining reference standard extracts, could be used for the authentication and quality control of bilberry extracts.

scholarly journals Analysis of the High-Performance Liquid Chromatography Fingerprints and Quantitative Analysis of Multicomponents by Single Marker of Products of Fermented Cordyceps sinensis

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Li-hua Chen ◽  
Yao Wu ◽  
Yong-mei Guan ◽  
Chen Jin ◽  
Wei-feng Zhu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Performance ◽  
Cordyceps Sinensis ◽  
Array Detector ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

Fermented Cordyceps sinensis, the succedaneum of Cordyceps sinensis which is extracted and separated from Cordyceps sinensis by artificial fermentation, is commonly used in eastern Asia in clinical treatments due to its health benefit. In this paper, a new strategy for differentiating and comprehensively evaluating the quality of products of fermented Cordyceps sinensis has been established, based on high-performance liquid chromatography (HPLC) fingerprint analysis combined with similar analysis (SA), hierarchical cluster analysis (HCA), and the quantitative analysis of multicomponents by single marker (QAMS). Ten common peaks were collected and analysed using SA, HCA, and QAMS. These methods indicated that 30 fermented Cordyceps sinensis samples could be categorized into two groups by HCA. Five peaks were identified as uracil, uridine, adenine, guanosine, and adenosine, and according to the results from the diode array detector, which can be used to confirm peak purity, the purities of these compounds were greater than 990. Adenosine was chosen as the internal reference substance. The relative correction factors (RCF) between adenosine and the other four nucleosides were calculated and investigated using the QAMS method. Meanwhile, the accuracy of the QAMS method was confirmed by comparing the results of that method with those of an external standard method with cosines of the angles between the groups. No significant difference between the two methods was observed. In conclusion, the method established herein was efficient, successful in identifying the products of fermented Cordyceps sinensis, and scientifically valid to be applicable in the systematic quality control of fermented Cordyceps sinensis products.

Characterization and distribution of bombesin-like peptides in the rat brain and gastrointestinal tract

1990 ◽  
Vol 68 (9) ◽  
pp. 1142-1145 ◽  
Author(s):  
Angel Hernanz
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Gastrointestinal Tract ◽  
Rat Brain ◽  
Retention Time ◽  
High Performance ◽  
Reverse Phase ◽  
Whole Brain ◽  
Gastrin Releasing Peptide ◽  
Peptide Peak

Extracts of rat brain and gastrointestinal tract, analyzed by reverse-phase high-performance liquid chromatography and radioimmunoassay, contained two bombesin-like immunoreactivity peaks with similar retention times as porcine gastrin-releasing peptide (GRP) and its COOH-terminal decapeptide, neuromedin C or GRP(18–27). However, the GRP-like peptide peak did not elute with exactly the same retention time as porcine GRP. The highest concentration of bombesin-like immunoreactivity was found in extracts of antrum, whereas the lowest was found in whole brain. Neuromedin C was present at lower concentrations than the GRP in antrum, duodenum, and ileum, while similar amounts of each were found in brain.Key words: gastrin-releasing peptide, neuromedin C, rat, brain, intestine.

Corticotrophin-releasing factor immunoreactivity in the hypothalamus of the rat during the perinatal period

1988 ◽  
Vol 119 (1) ◽  
pp. 59-64 ◽  
Author(s):  
A. Chatelain ◽  
F. Boudouresque ◽  
T. Chautard ◽  
J. P. Dupouyt ◽  
C. Oliver
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Median Eminence ◽  
Retention Time ◽  
High Performance ◽  
Perinatal Period ◽  
Adult Rats ◽  
Corticotrophin Releasing Factor ◽  
Specific Radioimmunoassay ◽  
Hypothalamic Control

ABSTRACT Corticotrophin-releasing factor-41 (CRF-41) immunoreactivity has been measured in hypothalamic extracts of fetal (on days 17, 19 and 21 of gestation), neonatal (1, 2, 3 and 4 weeks of age) and adult rats with a specific radioimmunoassay developed for synthetic rat CRF-41. The hypothalamic content (fmol) and concentration (fmol/mg protein) of immunoreactive CRF-41 gradually increased with age. Chromatography of hypothalamic extracts on Sephadex G-50 Fine showed one single peak of immunoreactive CRF-41 which co-eluted with synthetic rat CRF-41. The retention time of hypothalamic CRF-41 during high-performance liquid chromatography was identical to that of synthetic rat CRF-41 at all stages investigated. These results are consistent with the development of neurones containing CRF-41-like molecules in both the hypothalamus and the median eminence of the fetus, as well as with the hypothalamic control of the cortico-stimulating function of the pituitary gland as early as day 19 of gestation. J. Endocr. (1988) 119, 59–64

scholarly journals Senyawa Antibakteri Escherichia coli ATCC 35218 dan Staphylococcus aureus ATCC 25923 dari Kapang Endofit Taman Nasional Gunung Halimun

2012 ◽  
Vol 12 (1) ◽  
pp. 21 ◽  
Author(s):  
Ruth Melliawati ◽  
Harni Harni
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Retention Time ◽  
Endophytic Fungus ◽  
High Performance ◽  
Agar Plate ◽  
Chloroform Fraction ◽  
Antibacterial Compounds

Endophytic fungus is a microorganism which live in the interstitial spaces healthy tissues of the host plant, andhas capability to produce secondary metabolite such as micotoxin, antibiotic and antiviral. This research wasaimed to find out the isolates of endophytic fungus produce antibacterial compounds to inhibit Escerichia coliATCC 35218 and Staphylococcus aureus ATCC 25923, and to investigate the Retention time (Rt) of the antibacterialcompounds produced by endophytic fungus with High Performance Liquid Chromatography (HPLC) methods.Diffusion Agar Plate Method was used to examine the antibacterial compounds on Escerichia coli ATCC 35218 andStaphylococcus aureus ATCC 25923. While the antibacterial compounds were examined with Thin LayerChromatography (TLC) and High Performance Liquid Chromatography (HPLC) methods, and the result werecompared with Chloramphenicol and Ampicillin antibiotic standart. Two isolates of endophytic fungus namelyHl.46F.211 and HL.57F.258 were inhibited the growth Escherichia coli ATCC 35218 and three isolates namelyHL.48F217, HL.53F.243 and HL.57F.258 showed antagonistic action against Staphylococcus aureus ATCC 25923.The results of TLC and HPLC analysing method show that antibacterial compounds produced by endophytic fungusHL.46F.211 had Rt (Retention Time) rate similar with antibiotic Chloramphenicol at 2,796 (at water fraction) and Rtantibiotic Amphycillin at 2,662 (at Chloroform fraction). While antibacterial compounds produced by endophyticfungus HL.57F.258 had Rt rate similar with antibiotic Amphycillin at 2,650 (at Chloroform fraction).

Sign in / Sign up

Export Citation Format

Share Document